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Registros recuperados: 28
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Cancer stem cells - the current status of an old concept: literature review and clinical approaches Biol. Res.
Fulawka,Lukasz; Donizy,Piotr; Halon,Agnieszka.
As regards their morphology and biology, tumours consist of heterogeneous cell populations. The cancer stem cell (CSC) hypothesis assumes that a tumour is hierarchically organized and not all of the cells are equally capable of generating descendants, similarly to normal tissue. The only cells being able to self-renew and produce a heterogeneous tumour cell population are cancer stem cells. CSCs probably derive from normal stem cells, although progenitor cells may be taken into consideration as the source of cancer stem cells. CSCs reside in the niche defined as the microenvironment formed by stromal cells, vasculature and extracellular matrix. The CSC assays include FACS sorting, xenotransplantation to immunodeficient mice (SCID), incubation with Hoechst...
Tipo: Journal article Palavras-chave: Cancer stem cells; Stem cells; Tumour-initiating cells; Tumour-propagating cells; Carcinogenesis; Tumour heterogeneity; Clonal evolution.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100060
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Cell lineage relationship in the stomach of normal and genetically manipulated mice BJMBR
Karam,S.M..
The oxyntic mucosa of the mouse stomach is lined with a heterogeneous population of cells that form numerous short pits continuous with long tubular glands. Tritiated thymidine radioautography has made it possible to pinpoint the origin of all cell types and to follow the differentiation/migration of different cell lineages along the pit-gland unit. The proliferating multipotent stem cells functionally anchored in the upper glandular region, the isthmus, give rise to three main lineage precursors: 1) pre-pit cells, which migrate upward to the pit while differentiating into mucus-producing pit cells; 2) pre-neck cells, which migrate downward to the glandular neck while differentiating into mucus-producing neck cells that, by approaching the glandular base,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Radioautography; Gastric epithelium; Stem cells; Cell proliferation/differentiation programs; Lineage progenitors; Transgenic mice.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200010
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Clinical and experimental approaches to knee cartilage lesion repair and mesenchymal stem cell chondrocyte differentiation Biol. Res.
Montoya,Fredy; Martínez,Fernando; García-Robles,María; Balmaceda-Aguilera,Carolina; Koch,Ximena; Rodríguez,Federico; Silva-Álvarez,Carmen; Salazar,Katterine; Ulloa,Viviana; Nualart,Francisco.
Cartilage has poor regeneration capacity due to the scarcity of endogenous stem cells, its low metabolic activity and the avascular environment. Repair strategies vary widely, including microfracture, autologous or allogenic tissue implantation, and in vitro engineered tissues of autologous origin. However, unlike the advances that have been made over more than two decades with more complex organs, including vascular, cardiac or bone tissues, similar advances in tissue engineering for cartilage repair are lacking. Although the inherent characteristics of cartilage tissue, such as the lack of vascularity and low cellular diversity, suggest that it would be one of the more simple tissues to be engineered, its functional weight-bearing role and implant...
Tipo: Journal article Palavras-chave: Cartilage; Lesion; Microfracture; Stem cells.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400015
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Correlation between Innervation of Skeletal Muscles and Myoblast Stem Cells International Journal of Morphology
Wu,Jiang; Sun,Xiaojuan; Zhu,Min; Zhu,Chongtao; Chen,Bin; Xu,Yongqing; Ouyang,Jun; Liao,Hua; Qiu,Xiaozhong.
In order to explore the change rule of myoblast stem cells (satellite cells, SCs) in the denervated and re-innervated muscle and to investigate the cellular mechanism of the morphological and functional changes of the muscle, denervated muscle atrophy and nerve regeneration models were established in one-month-old rats. Postoperative indexes such as muscle wet weight, cell section areas, content of collagen fibers and DNA, electrophysiology, numbers of SCs in the triceps muscle of calf were dynamically tested. After denervation, the muscle wet weight and cell area reduced rapidly, and the collagen fiber content increased slowly. The number of SCs increased at first, and then declined suddenly two months later. From 4 to 5 weeks after re-neuralization,...
Tipo: Journal article Palavras-chave: Satellite cells; Atrophy; Denervation; Innervations; Stem cells.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400043
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Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom? Ciência Rural
Telles,Thalita da Costa; Botelho,Ana Flávia Machado; Miranda,Ana Luisa Soares de; Soto-Blanco,Benito; Serakides,Rogéria; Ocarino,Natália de Melo; Melo,Marília Martins.
ABSTRACT: The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120μg of B. alternatus venom, while G3 received 200μL of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 106 MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Viperidae envenomation; Stem cells; Rats; Myonecrosis; Muscular repair.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602
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Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model BJMBR
Wang,Z.H.; Li,X.L.; He,X.J.; Wu,B.J.; Xu,M.; Chang,H.M.; Zhang,X.H.; Xing,Z.; Jing,X.H.; Kong,D.M.; Kou,X.H.; Yang,Y.Y..
SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic modification; Tissue engineering; Stem cells; Sox9; Chondrogenesis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400279
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Development of VEGF-loaded PLGA matrices in association with mesenchymal stem cells for tissue engineering BJMBR
Rosa,A.R.; Steffens,D.; Santi,B.; Quintiliano,K.; Steffen,N.; Pilger,D.A.; Pranke,P..
The association of bioactive molecules, such as vascular endothelial growth factor (VEGF), with nanofibers facilitates their controlled release, which could contribute to cellular migration and differentiation in tissue regeneration. In this research, the influence of their incorporation on a polylactic-co-glycolic acid (PLGA) scaffold produced by electrospinning on cell adhesion and viability and cytotoxicity was carried out in three groups: 1) PLGA/BSA/VEGF; 2) PLGA/BSA, and 3) PLGA. Morphology, fiber diameter, contact angle, loading efficiency and controlled release of VEGF of the biomaterials, among others, were measured. The nanofibers showed smooth surfaces without beads and with interconnected pores. PLGA/BSA/VEGF showed the smallest water contact...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Emulsion electrospinning; VEGF; Stem cells; PLGA; Biomaterials.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900601
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Early Proliferation of Bone Marrow Mononuclear Cells on Collagen Membrane, Bone Graft and Tooth Cementum International Journal of Morphology
Milagres,Débora; Rueff-Barroso,Carlos R; Carvalho,Simone N; Carvalho,Laís de; Monte-Alto-Costa,Andréa; Fischer,Ricardo G; Porto,Luís Cristóvão.
The ultimate goal of periodontal therapy is to repair the damaged periodontal supporting tissues, permitting regeneration of the periodontal ligament. However, the cell response, the supportive matrix and the bioactive molecules use have not yet been well established. Bone marrow mononuclear cells were extracted from rat femurs and tibiae and cultured on a cross-linked collagen membrane, bone graft, or molar tooth to compare cell attachment and early proliferation on these materials. Cell attachment was quantified by light microscopy at 24, 48 and 72h, and cell proliferation was observed under a SEM after 72h. After 24h, the number of cells on bone graft was similar to that of the control and more than twice compared to collagen membrane (q=7.473...
Tipo: Journal article Palavras-chave: Stem cells; Collagen membrane; Bone graft; Cementum; Proliferation.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400045
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Ectopic development of skeletal muscle induced by subcutaneous transplant of rat satellite cells BJMBR
Fukushima,M.G.; Furlan,I.; Chiavegatti,T.; Kiyomoto,B.H.; Godinho,R.O..
The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (10(6) cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodamine-labeled alpha-bungarotoxin. The development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Skeletal muscle; Satellite cells; Stem cells; Transplantation; Myogenesis; 2BAW rats.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300007
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Evo-devo of non-bilaterian animals Genet. Mol. Biol.
Lanna,Emilio.
The non-bilaterian animals comprise organisms in the phyla Porifera, Cnidaria, Ctenophora and Placozoa. These early-diverging phyla are pivotal to understanding the evolution of bilaterian animals. After the exponential increase in research in evolutionary development (evo-devo) in the last two decades, these organisms are again in the spotlight of evolutionary biology. In this work, I briefly review some aspects of the developmental biology of nonbilaterians that contribute to understanding the evolution of development and of the metazoans. The evolution of the developmental genetic toolkit, embryonic polarization, the origin of gastrulation and mesodermal cells, and the origin of neural cells are discussed. The possibility that germline and stem cell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Early-diverging metazoans; BMP; FGF; Wnt; Stem cells; Evolution; Nervous system.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300284
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Hematopoietic stem cell transplantation: clinical use and perspectives Biol. Res.
Barriga,Francisco; Ramírez,Pablo; Wietstruck,Angélica; Rojas,Nicolás.
Hematopoietic stem cell transplantation is the accepted therapy of choice for a variety of malignant and non-malignant diseases in children and adults. Initially developed as rescue therapy for a patient with cancer after high doses of chemotherapy and radiation as well as the correction of severe deficiencies in the hematopoietic system, it has evolved into an adoptive immune therapy for malignancies and autoimmune disorders. The procedure has helped to obtain key information about the bone marrow environment, the biology of hematopoietic stem cells and histocompatibility. The development of this new discipline has allowed numerous groups working around the world to cure patients of diseases previously considered lethal. Together with the ever growing...
Tipo: Journal article Palavras-chave: Hematopoietic; Stem cells; Transplantation; Allogeneic.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300012
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Human dental pulp stem cells transplantation combined with treadmill training in rats after traumatic spinal cord injury BJMBR
Nicola,F.C.; Rodrigues,L.P.; Crestani,T.; Quintiliano,K.; Sanches,E.F.; Willborn,S.; Aristimunha,D.; Boisserand,L.; Pranke,P.; Netto,C.A..
Spinal cord injury (SCI) is a disabling condition resulting in deficits of sensory and motor functions, and has no effective treatment. Considering that protocols with stem cell transplantation and treadmill training have shown promising results, the present study evaluated the effectiveness of stem cells from human exfoliated deciduous teeth (SHEDs) transplantation combined with treadmill training in rats with experimental spinal cord injury. Fifty-four Wistar rats were spinalized using NYU impactor. The rats were randomly distributed into 5 groups: Sham (laminectomy with no SCI, n=10); SCI (laminectomy followed by SCI, n=12); SHEDs (SCI treated with SHEDs, n=11); TT (SCI treated with treadmill training, n=11); SHEDs+TT (SCI treated with SHEDs and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spinal cord injury; Stem cells; Treadmill training; Functional recovery; MASCIS impactor.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000900604
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Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha) Arq. Bras. Med. Vet. Zootec.
Rocha,A.R.; Leite,Y.K.C.; Silva,A.S.; Conde Júnior,A.M.; Costa,C.R.M.; Silva,G.C.; Bezerra,D.O.; Cavalcante,M.M.A.S.; Feitosa,M.L.T.; Argôlo Neto,N.M.; Serakides,R.; Carvalho,M.A.M..
ABSTRACT There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell culture; Mesenchymal; Stem cells; Adipose tissue; Cell differentiation; Fluorescent nanocrystals.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501571
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Maintenance of the corneal epithelium is carried out by germinative cells of its basal stratum and not by presumed stem cells of the limbus BJMBR
Haddad,A.; Faria-e-Sousa,S.J..
The purpose of this investigation was to analyze the proliferative behavior of rabbit corneal epithelium and establish if any particular region was preferentially involved in epithelial maintenance. [3H]-thymidine was injected intravitreally into both normal eyes and eyes with partially scraped corneal epithelium. Semithin sections of the anterior segment were evaluated by quantitative autoradiography. Segments with active replication (on) and those with no cell division (off) were intermingled in all regions of the tissue, suggesting that the renewal of the epithelial surface of the cornea followed an on/off alternating pattern. In the limbus, heavy labeling of the outermost layers was observed, coupled with a few or no labeled nuclei in the basal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cornea; Epithelium; Autoradiography; Germinative cells; Stem cells; Limbus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000600470
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Microencapsulation and tissue engineering as an alternative treatment of diabetes BJMBR
Maria-Engler,S.S.; Mares-Guia,M.; Correa,M.L.C.; Oliveira,E.M.C.; Aita,C.A.M.; Krogh,K.; Genzini,T.; Miranda,M.P.; Ribeiro,M.; Vilela,L.; Noronha,I.L.; Eliaschewitz,F.G.; Sogayar,M.C..
In the 70's, pancreatic islet transplantation arose as an attractive alternative to restore normoglycemia; however, the scarcity of donors and difficulties with allotransplants, even under immunosuppressive treatment, greatly hampered the use of this alternative. Several materials and devices have been developed to circumvent the problem of islet rejection by the recipient, but, so far, none has proved to be totally effective. A major barrier to transpose is the highly organized islet architecture and its physical and chemical setting in the pancreatic parenchyma. In order to tackle this problem, we assembled a multidisciplinary team that has been working towards setting up the Human Pancreatic Islets Unit at the Chemistry Institute of the University of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human pancreatic islets; Pancreas; Extracellular matrix; Stem cells; Islet microencapsulation; Transplantation; Ductal cells.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600001
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Nestin-positive Stem Cells Participate in Chondrocyte Renewal in Healthy Adult Lung Cartilage International Journal of Morphology
Ortega-Martínez,Marta; de-la-Garza-González,Carlos; Ancer-Rodríguez,Jesús; Jaramillo-Rangel,Gilberto.
It is considered that healthy adult cartilage has little or no capacity for renewal, and that chondrocytes maintain a stable resting phenotype and resist proliferation and differentiation throughout life. Recently we found that cell turnover in lung cartilage is possible and that nestin-positive cells may have a role in it. In this paper, we report additional findings about chondrocyte renewal in lung cartilage. Lung specimens from CD1 mice at the age of 2, 6, 12, 18 or 24 months were fixed in 10% neutral-buffered formalin and paraffin-embedded. Nestin expression was examined by an immunohistochemical peroxidase-based method. We found nestin-positive cells inside of cartilage islets and cells in division very close from them. Our findings indicate that...
Tipo: Journal article Palavras-chave: Cartilage; Chondrocyte renewal; Nestin; Stem cells.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100026
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Neuromuscular disorders: genes, genetic counseling and therapeutic trials Genet. Mol. Biol.
Zatz,Mayana; Passos-Bueno,Maria Rita; Vainzof,Mariz.
Abstract Neuromuscular disorders (NMD) are a heterogeneous group of genetic conditions, with autosomal dominant, recessive, or X-linked inheritance. They are characterized by progressive muscle degeneration and weakness. Here, we are presenting our major contributions to the field during the past 30 years. We have mapped and identified several novel genes responsible for NMD. Genotype-phenotype correlations studies enhanced our comprehension on the effect of gene mutations on related proteins and their impact on clinical findings. The search for modifier factors allowed the identification of a novel "protective"; variant which may have important implication on therapeutic developments. Molecular diagnosis was introduced in the 1980s and new technologies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diseases; Genetic counseling; Neuromuscular disorders; Stem cells; Therapies.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300339
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Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. Repositório Alice
PILLAI, VIJU VIJAYAN; SIQUEIRA, L. G. B.; DAS, MOUBANI; KEI, TIFFANY G.; TU, LAN N.; HERREN, ANTHONY W.; PHINNEY, BRETT S.; CHEONG, SOON HON; HANSEN, PETER J.; SELVARAJ, VIMAL.
bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Placenta; Trophoblast; Blastocyst; Stem cells; Embryo implantation; Pregnancy.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117829
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Prolonged exposure of human embryonic stem cells to heat shock induces necrotic cell death Biocell
Heng,Boon Chin; Vinoth,Kumar Jayaseelan; Lu,Kai; Deng,Xuliang; Ge,Zigang; Bay,Boon Huat; Cao,Tong.
We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane. This was further confirmed by flow cytometry analysis which showed that the DNA of the dead/ dying cells was still mostly intact, unlike...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryonic; Heat shock; Human; Necrosis; Stem cells.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000300007
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Restoration of the rabbit corneal surface after total epithelial debridement and complete limbal excision BJMBR
Faria-e-Sousa,S.J.; Haddad,A..
How is the corneal epithelium restored when all of it plus the limbus have been eliminated? This investigation explored the possibility that this may be achieved through the conjunctival epithelium. The corneal epithelium of the right eye of 12 rabbits (Oryctolagus cuniculus) was totally scraped followed by surgical excision of the limbus plus 1.0-1.5 mm of the adjacent conjunctiva. Antibiotics and corticosteroids were applied for 1 week after surgery. Histological and immunohistochemical techniques were used to monitor the events taking place on the eye surface 2 weeks and 1, 3 and 6 months thereafter. Initially, the corneal surface was covered by conjunctival-like epithelium. After 1 month and more prominently at 3 and 6 months an epithelium displaying...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Corneal epithelium; Conjunctiva; Stem cells; Epithelium transformation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500005
Registros recuperados: 28
Primeira ... 12 ... Última
 

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