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Characterization of rat heart alkaline phosphatase isoenzymes and modulation of activity BJMBR
Mota,A.; Silva,P.; Neves,D.; Lemos,C.; Calhau,C.; Torres,D.; Martel,F.; Fraga,H.; Ribeiro,L.; Alçada,M.N.M.P.; Pinho,M.J.; Negrão,M.R.; Pedrosa,R.; Guerreiro,S.; Guimarães,J.T.; Azevedo,I.; Martins,M.J..
Alkaline phosphatase (ALP) is important in calcification and its expression seems to be associated with the inflammatory process. We investigated the in vitro acute effects of compounds used for the prevention or treatment of cardiovascular diseases on total ALP activity from male Wistar rat heart homogenate. ALP activity was determined by quantifying, at 410 nm, the p-nitrophenol released from p-nitrophenylphosphate (substrate in Tris buffer, pH 10.4). Using specific inhibitors of ALP activity and the reverse transcription-polymerase chain reaction, we showed that the rat heart had high ALP activity (31.73 ± 3.43 nmol p-nitrophenol·mg protein-1·min-1): mainly tissue-nonspecific ALP but also tissue-specific intestinal ALP type II. Both ALP isoenzymes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heart; Alkaline phosphatase; Polyphenol-rich beverages; Steroid hormones; Methylxanthines.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700009
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In vitro modulation of alkaline phosphatase activity of Saccharomyces cerevisiae grown in low or high phosphate medium BJMBR
Fernandes,J; Amorim,R; Azevedo,I; Martins,M.J.
Our objective was to characterize the modulation of the activity of Saccharomyces cerevisiae alkaline phosphatases (ALPs) by classic inhibitors of ALP activity, cholesterol and steroid hormones, in order to identify catalytic similarities between yeast and mammalian ALPs. S. cerevisiae expresses two ALPs, coded for by the PHO8 and PHO13 genes. The product of the PHO8 gene is repressible by Pi in the medium. ALP activity from yeast (grown in low or high phosphate medium) homogenates was determined with p-nitrophenylphosphate as substrate, pH 10.4 (lPiALP or hPiALP, respectively). Activation of hPiALP was observed with 5 mM L-amino acids (L-homoarginine _ 186%, L-leucine _ 155% and L-phenylalanine - 168%) and with 1 mM levamisole (122%; percentage values, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Alkaline phosphatase inhibitors; PHO8 and PHO13; Cholesterol; Steroid hormones.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000100007
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Modulation by steroid hormones of a ''sexy'' acoustic signal in an Oscine species, the Common Canary Serinus canaria Anais da ABC (AABC)
Rybak,Fanny; Gahr,Manfred.
The respective influence of testosterone and estradiol on the structure of the Common Canary Serinus canaria song was studied by experimentally controlling blood levels of steroid hormones in males and analyzing the consequent effects on acoustic parameters. A detailed acoustic analysis of the songs produced before and after hormonal manipulation revealed that testosterone and estradiol seem to control distinct song parameters independently. The presence of receptors for testosterone and estradiol in the brain neural pathway controlling song production strongly suggests that the observed effects are mediated by a steroid action at the neuronal level.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oscines; Common Canary; Song; Steroid hormones.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000200026
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Receptor localization of steroid hormones and drugs: discoveries through the use of thaw-mount and dry-mount autoradiography BJMBR
Stumpf,W.E..
The history of receptor autoradiography, its development and applications, testify to the utility of this histochemical technique for localizing radiolabeled hormones and drugs at cellular and subcellular sites of action in intact tissues. Localization of diffusible compounds has been a challenge that was met through the introduction of the "thaw-mount" and "dry-mount" autoradiographic techniques thirty years ago. With this cellular receptor autoradiography, used alone or combined with other histochemical techniques, sites of specific binding and deposition in vivo and in vitro have been characterized. Numerous discoveries, some reviewed in this article, provided information that led to new concepts and opened new areas of research. As an example, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitamin D; Steroid hormones; Receptors; Autoradiography; Drug distribution; Localization.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200003
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