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Registros recuperados: 17
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Alterations of the Antioxidant Enzyme Activities are not General Characteristics of the Colonization Process by Arbuscular Mycorrhizal Fungi Chilean J. Agric. Res.
Rodríguez,Yakelin; Vierheilig,Horst; Mazorra,Luis M.
Antioxidant system is involved in arbuscular mycorrhizal symbiosis, but its role during the colonization process is still poorly understood. To gain new insights into the role of antioxidant system during root colonization by arbuscular mycorrhizal fungi, the activities of key antioxidant enzymes were evaluated in tomato (Solanum lycopersicum L.) roots inoculated with six strains of different genera and species: two Glomus mosseae, Glomus cubense, Glomus intraradices, Glomus sp. and Acaulospora scrobiculata. Glomus cubense and A. scrobiculata strains reached the highest infectivity levels with maximum values of colonization frequency and intensity of 29-10.88% and 18-9.20%, respectively; G. mosseae strains showed an intermediate infectivity, both with 15%...
Tipo: Journal article Palavras-chave: Peroxidases; Polyphenol oxidases; Superoxide dismutase; Colonization intensity; Solanum lycopersicum.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300016
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Antioxidant capacity in relationship to serum lipid peroxides levels in healthy elderly of Mexico City ABCL
Sánchez-Rodríguez,Martha A.; Retana-Ugalde,Raquel; Ruiz-Ramos,Mirna; Mendoza-Núñez,Víctor Manuel.
The aging is one of the factors that cause decrease in the antioxidant capacity. Likewise, it has been proposed that subjects exposed permanently to air pollution develop deficient antioxidant capacity to oxidative stress (OxS). This study aimed to analyze the antioxidant capacity against elevated lipid peroxides in healthy elderly of Mexico City. 105 adults (44 ± 10.8 years) and 126 elderly subjects were studied (68 ± 7.1 years); residents of Mexico City (clinically healthy, non-smokers, non-vitamin supplement takers) who had lived in the city for >10 years. Plasma lipoperoxides (LPO), total antioxidant status (TAS), the activity of red blood cells superoxide dismutase (SOD), and plasma glutathione peroxidase (GPx), were studied in all subjects. LPO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation to oxidative stress; Total antioxidant status; Elderly people; Pollution; Superoxide dismutase; Glutathione peroxidase.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572004000200007
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Application of various antioxidants in the treatment of influenza BJMBR
Dolganova,A.; Sharonov,B.P..
We determined the effect of the antioxidants superoxide dismutase, desferrioxamine and allopurinol on the survival of male CBA mice infected intranasally with 2-5 LD50 lung influenza virus A/Aichi/2/68. Survival for at least 20 days was observed for 45% of the mice that received 1000 U/day superoxide dismutase prepared from red blood cells on days 5, 6, 7 and 8 after infection, and 75% survival was observed for mice that received the same dose on days 4, 5, 6, 7 and 8. Desferrioxamine, 25 mg/kg per day and 100 mg/kg per day injected subcutaneously, resulted in survival rates of 5 and 0%, respectively, compared to 10% survival observed for saline-injected controls. Allopurinol at doses of 5 to 50 mg/kg per day had no effect on mouse survival. These data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Free radicals; Influenza; Antioxidant; Desferrioxamine; Superoxide dismutase; Allopurinol.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100012
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Changes in biochemical characteristics and Na and K content of caper (Capparis spinosa L.) seedlings under water and salt stress JARTS
Sadeghi, Hossein; Rostami, Laleh.
In order to investigate the effect of water and salt stress on caper (Capparis spinosa L.) seedlings, a randomized complete block design with five replications was carried out in 2013 at Shiraz University, Iran. Water stress had three levels: 100 % (control), 75 %, and 5 % field capacity (FC), and five levels of salinity were applied: 0 (control), 4, 8, 12, and 18 dSm^(−1). The results indicated that salinity had a significantly negative effect on chlorophyll content of caper seedlings, while drought increased this content. The carotenoid content in caper seedlings under water and salinity stress was significantly increased. Proline and total protein content increased also under both salinity and water stress. Antioxidant enzyme activity; superoxide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant Ecophysiology caper; Catalase; Peroxidase; Salt stress; Superoxide dismutase.
Ano: 2017 URL: http://www.jarts.info/index.php/jarts/article/view/2017062752895
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Changes in the TBARs content and superoxide dismutase, catalase and glutathione peroxidase activities in the lymphoid organs and skeletal muscles of adrenodemedullated rats BJMBR
Pereira,B.; Costa-Rosa,L.F.B.P.; Bechara,E.J.H.; Newsholme,P.; Curi,R..
Thiobarbituric acid reactant substances (TBARs) content, and the activities of glucose-6-phosphate dehydrogenase (G6PDh), citrate synthase (CS), Cu/Zn- and Mn-superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were measured in the lymphoid organs (thymus, spleen, and mesenteric lymph nodes (MLN)) and skeletal muscles (gastrocnemius and soleus) of adrenodemedullated (ADM) rats. The results were compared with those obtained for sham-operated rats. TBARs content was reduced by adrenodemedullation in the lymphoid organs (MLN (28%), thymus (40%) and spleen (42%)) and gastrocnemius muscle (67%). G6PDh activity was enhanced in the MLN (69%) and reduced in the spleen (28%) and soleus muscle (75%). CS activity was reduced in all tissues (MLN...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epinephrine; Lymphoid organs; Superoxide dismutase; Catalase; Glutathione peroxidase; TBARs.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600016
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Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat ArchiMer
Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf
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Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens ArchiMer
Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf
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Evaluation of toxicity diflubenzuron and p-chloroaniline in biochemical indicators of fishes Oreochromis niloticus. Repositório Alice
DANTZGER, D. D.; ANDRADE, C. A.; ANJOS, E. F.; JONSSON, C. M.; AOYAMA, H..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Diflubenzuron; P-chloroaniline; Toxicity; Oreochromis niloticus; Acid and alkaline phosphatases; Catalase; Superoxide dismutase.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/982558
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Gene expression of antioxidant enzymes and coffee seed quality during pre-and post- physiological maturity. Repositório Alice
SANTOS, F. C.; CAIXETA, F.; CLEMENTE, A. C. S.; PINHO, E. V.; ROSA, S. D. V. F. da.
Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre- and post-physiological maturation phases in whole seeds and in endosperms and embryos extracted from the seeds. Coffea arabica L. berries were collected at the green, yellowish-green, cherry, over-ripe, and dry stages, and the seeds were examined physiologically. The transcription levels of the genes were quantified by quantitative real-time polymerase chain reaction using coffee-specific primers. The highest level of SOD expression was observed in the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Catalace; Superoxide dismutase; Catechol oxidase.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1039772
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Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection ArchiMer
Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne.
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection....
Tipo: Text Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00221/33257/31733.pdf
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Metabolismo antioxidante de pedúnculos e frutos de Coffea arabica L. com diferentes forças de desprendimento. Repositório Alice
BRANDÃO, I. R.; SILVA, D. M. da; SOUZA, K. R. D. de; VILAS BOAS, L. V.; SILVA, F. M. da; CARVALHO, M. A. de F.; ALVES, J. D..
O objetivo deste trabalho foi investigar o metabolismo antioxidante de pedúnculos e frutos de Coffea arabica em diferentes estádios de maturação e forças de desprendimento. Frutos e pedúnculos de cafeeiro foram coletados com auxílio de um dinamômetro portátil em diferentes estádios de maturação e forças de desprendimento (10 ? 13N e 8 ? 9,9N, 6 ? 6,9N e 5 ? 5,9N, 4 ? 4,9 e 2 ? 3,9N). Após a coleta o material vegetal foi separado em cada intervalo de força pré-determinado e colocado em nitrogênio líquido até o armazenamento em ultrafreezer. Foram quantificados os níveis de peróxido de hidrogênio, peroxidação lipídica e a atividade das enzimas dismutase do superóxido, catalase e peroxidase do ascorbato. Maiores níveis de peróxido de hidrogênio foram...
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Dismutase do superóxido; Peroxidase do ascorbato; Fruit ripening; Catalase; Maturação; Fruto; Superoxide dismutase; Catalase; Ascorbate peroxidase.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1041515
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Molecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought. Repositório Alice
DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P..
Drought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Enzymatic activity; Arroz; Oryza sativa; Resistencia a seca; Rice; Water stress; Oxidative stress; Gene expression; Superoxide dismutase.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1037521
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Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas ArchiMer
Harney, Ewan; Artigaud, Sebastien; Le Souchu, Pierrick; Miner, Philippe; Corporeau, Charlotte; Essid, Hafida; Pichereau, Vianney; Nunes, Flavia.
Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40 h under either control (pH 8.1, 20 °C), acidified (pH 7.9, 20 °C), warm (pH 8.1, 22 °C) or warm acidified (pH 7.9, 20 °C) conditions. Larvae in acidified conditions were significantly smaller than in the control, but warm acidified conditions mitigated negative effects on size, and increased calcification. A proteomic approach employing two-dimensional electrophoresis (2-DE) was used to quantify proteins and relate their abundance to phenotypic traits. In...
Tipo: Text Palavras-chave: Crassostrea gigas; Ocean acidification; Larval development; ATP synthase; GAPDH; Superoxide dismutase.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00299/40985/40076.pdf
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Photosynthetic and antioxidant performance are differentially affected by short-term nitrogen supply in highbush blueberry cultivars Ciencia e Investigación Agraria
Yañez-Mansilla,Erwin; Cartes,Paula; Reyes-Díaz,Marjorie; Ribera-Fonseca,Alejandra; Alberdi,Miren.
Nitrogen (N) is an essential nutrient for photosynthesis and may influence phenolic compound synthesis in higher plants. The effect of different amounts of N (0 to 38 mM) provided on a short-term (4 d) basis on the photosynthetic and antioxidant performance of highbush blueberry cultivars (Legacy and Bluegold) grown in a nutrient solution was studied. In both cultivars, the N concentration of leaves slightly increased in response to the N supply, with Bluegold frequently showing higher N concentrations than Legacy. Photosynthesis was reduced in Bluegold at the highest N dose, whereas in Legacy, a decrease of CO2 assimilation occurred under N starvation. This decrease in photosynthesis was accompanied by enhanced lipid peroxidation but only in Bluegold. In...
Tipo: Journal article Palavras-chave: Nitrogen; Oxidative damage; Phenolic compounds; Superoxide dismutase; Vaccinium corymbosum.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000100006
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Seasonal changes in mRNA encoding for cell stress markers in the oyster Crassostrea gigas exposed to radioactive discharges in their natural environment ArchiMer
Farcy, Emilie; Voiseux, Claire; Lebel, Jean-marc; Fievet, Bruno.
The North Cotentin area (Normandy, France) hosts several nuclear facilities among which the AREVA reprocessing plant of La Hague is responsible for controlled discharges of liquid radioactive wastes into the marine environment. The resulting increase in radioactivity is very small compared to natural radioactivity. However, concerns about environment protection prompted the scientific community to focus on the effects of the chronic exposure to low concentrations of radionuclides in non-human biota. This study contributes to the evaluation of the possible impact of radioactive discharges on the oyster Crassostrea gigas in the field. Real-time polymerase chain reaction was used to quantify the expression levels of genes involved in cell stress in the...
Tipo: Text Palavras-chave: Crassostrea gigas; Superoxide dismutase; Metallothionein; Heat shock protein; MRNA; Seasonal variations; Radioactive discharges.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2601.pdf
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Superoxide dismutase and catalase: tissue activities and relation with age in the long-lived species Margaritifera margaritifera Biol. Res.
FERNÁNDEZ,CARLOS; SAN MIGUEL,EDUARDO; FERNÁNDEZ-BRIERA,ALMUDENA.
Free radicals are extremely reactive and produce damage and modify cell functions. Furthermore, superoxide dismutase and catalase are believed to play a key role in the enzymatic defence of the cells. Indeed, some authors have argued that reduced free-radical damage could explain increased longevity. Margaritifera margaritifera is one of the longest-lived animals in the world (up to 100-200 years). Furthermore, this organism may serve as a useful model for gerontologists interested in exploring the mechanisms that promote long life and the slowing of senescence. The present study estimated for the first time individual enzymatic activity for superoxide dismutase isozymes (Cu,Zn-SOD and Mn-SOD) and catalase in tissue preparations of gills, digestive glands...
Tipo: Journal article Palavras-chave: Margaritifera margaritifera; Free radicals; Aging; Superoxide dismutase; Catalase.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100006
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กิจกรรมเอนไซม์ซูเปอร์ออกไซด์ดิสมิวเทสในหัวแก่นตะวันสายพันธุ์ต่างๆ Thai Agricultural
Anoma Dongsansuk; Sanan Jogloy.
The study of SOD activity in Jerusalem artichoke tubers in 3 varieties that were different harvesting stage and no report the amount of SOD in different parts of Jerusalem artichoke tuber was carried out. The aims of this study were to evaluate SOD activity in various varieties of Jerusalem artichoke and to evaluate the difference of SOD activity in different parts of Jerusalem artichoke tuber. This experiment used 3 varieties of Jerusalem artichoke including Hel 65, CN 52867 and JA 89 that were different genotypes. These tubers were separated into 3 parts: head, middle and tail for extraction and determination of SOD activity. The results showed that Jerusalem artichoke var. Hel 65 exhibited the highest SOD activity compared to var. CN 52867 and JA 89....
Tipo: PhysicalObject Palavras-chave: Helainthus tuberosus; Jurusalem artichoke; Enzyme; Antioxidant enzyme; Superoxide dismutase; SOD; Genotypes; แก่นตะวัน; เยรูซาเลม อาร์ติโชค; การเปรียบเทียบพันธุ์; เอนไซม์; ซูเปอร์ออกไซด์ดิสมิวเทส; แอนติออกซิแดนท์.
Ano: 2014 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5735
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