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Characterization of alkaline lipase from Fusarium oxysporum and the effect of different surfactants and detergents on the enzyme activity BJM
Prazeres,Janaina Nicanuzia dos; Cruz,Juliana Aparecida Bortollotti; Pastore,Gláucia Maria.
Nowadays, there is a tendency of use of low temperature in laundry cleaning for both environmental and economical reasons, which makes the use of enzymes in detergent products indispensable. Since lipases are efficient catalyst both in solution and at the water-liquid interface, they are potentially suitable for lipid stain removal applications in industrial laundry and household detergents. The effect of different commercial detergents and surfactants on enzymatic activity of lipase from Fusarium oxysporum was observed through p-nitrophenylpalmitate (pNPP) assay. The enzyme was compatible with various ionic and non-ionic surfactants as well as commercial detergents. Lipase activity was strongly inhibited by Sodium Dodecyl Sulphate (SDS), but not by Triton...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline lipase; Fusarium oxysporum; Surfactants; Detergents.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400019
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Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria ArchiMer
Ortega Morales, Benjamín Otto; Santiago Garcia, J.l; Chan Bacab, M.j.; Moppert, Xavier; Miranda Tello, E; Fardeau, M.l.; Carrero, J.c.; Bartolo Perez, P.; Valadez Gonzalez, A; Guezennec, Jean.
Aims: This study was performed to determine the potential of tropical intertidal biofilm bacteria as a source of novel exopolymers (EPS). Methods and Results: A screening procedure was implemented to detect EPS-producing biofilm bacteria. Isolates MC3B-10 and MC6B-22, identified respectively as a Microbacterium species and Bacillus species by 16S rDNA and cellular fatty acids analyses, produced different EPS, as evidenced by colorimetric and gas chromatographic analyses. The polymer produced by isolate MC3B-10 displays significant surfactant activity, and may chelate calcium as evidenced by spectroscopic analysis. Conclusions: Polymer MC3B-10 appears to be a glycoprotein, while EPS MC6B-22 seems to be a true polysaccharide dominated by neutral sugars...
Tipo: Text Palavras-chave: Tropical intertidal biofilm bacteria; Surfactants; Microbacterium sp; Exopolymers; Bacillus sp; Anionic.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2530.pdf
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Diversity of ejaculated sperm proteins in Moxotó bucks (Capra hircus) evaluated by multiple extraction methods. Repositório Alice
MOREIRA, R. F.; MATOS, M. N. C.; ALVES FILHO, J. G.; VALLE, R. V. do; ELOY, A. M. X.; PINTO, T. M. F.; MACHADO, S. P.; COSTA, C. R. R.; LIMA FILHO, J. L. de; LIMA, J. P. M. S.; CUNHA, R. M. S. da..
Abstract: This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats (Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were isolated using an extraction buffer containing 7 M urea and 2 M thiourea, 20 mM DTT, and one of the following detergents: 1% or 4% CHAPS; 1% or 4% SDS; 1% or 4% Triton X-100; or a combination of CHAPS and SDS. The 1-DE and 2-DE profiles of the isolated proteins revealed that the various isolation methods were efficient. Qualitative and quantitative differences in the 1-DE and 2-DE profiles were observed. 2-DE maps indicated that the amount and diversity of proteins visualized depended on the detergent that was used. Furthermore, this work revealed that the combination of...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Isolation methods; Proteomic profiles; Proteomic analysis; Raça Moxotó; Análise proteômica; Caprino; Detergente; Goats; Surfactants; Spermatozoa; Detergents; Proteomics.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1090515
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Effect of urea on biomimetic aggregates BJMBR
The effect of urea on biomimetic aggregates (aqueous and reversed micelles, vesicles and monolayers) was investigated to obtain insights into the effect of the denaturant on structured macromolecules. Direct evidence obtained from light scattering (static and dynamic), monolayer maximum isothermal compression and ionic conductivity measurements, together with indirect evidence from fluorescence photodissociation, fluorescence suppression, and thermal reactions, strongly indicates the direct interaction mechanism of urea with the aggregates. Preferential solvation of the surfactant headgroups by urea results in an increase in the monomer dissociation degree (when applied), which leads to an increase in the area per headgroup and also in the loss of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Urea; Denaturation; Surfactants; Macromolecular structure.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200004
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Emulsões estabilizadas por celulose bacteriana nanofibrilada: efeito do grau de oxidação. Infoteca-e
ROSA, M. de F.; GOIANA, M. L.; PEREIRA, A. L. S.; MORAIS, J. P. S..
Emulsões Pickering são sistemas formados pela homogeneização de dois líquidos imiscíveis entre si, estabilizados por partículas sólidas coloidais. Partículas naturais estão sendo amplamente estudadas para redução ou substituição de surfactantes sintéticos, empregados como estabilizantes, devido aos seus efeitos adversos, incluindo toxicidade. Uma das fontes naturais promissoras é a celulose bacteriana, que, quando utilizada na forma de nanofibrilas, permite modificações nas propriedades de superfície e tamanho das micelas da emulsão por mecanismos físicos e químicos. No presente trabalho, celulose bacteriana nanofibrilada (CBNF), obtida por combinação de oxidação mediada por 2,2,6,6-tetrametilpiperidin-1-oxil (TEMPO) e fibrilação em moinho coloidal, foi...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Emulsões Pickering; Nanotecnologia; Oxidação TEMPO; Surfactantes; Pickering emulsions; TEMPO oxidation; Surfactants.
Ano: 2020 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1126272
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Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants BJM
Rakicka,Magdalena; Rywińska,Anita; Cybulski,Krzysztof; Rymowicz,Waldemar.
Abstract Various chemical compounds, including surfactants, when introduced to culture media may increase the permeability of cellular membranes and thereby affect the quantity of metabolites excreted by cells. The aim of the present study was to evaluate the impact of detergents including Triton X-100, Span 20 and Tween 80 on erythritol production from glycerol by Yarrowia lipolytica Wratislavia K1 in a shake-flask experiment, batch and fed-batch cultures. When Span 20 was added to a fed-batch culture with glycerol as a carbon source (300 g L-1), erythritol production increased by 15% compared to the culture without the surfactant where it reached 142 g L-1 after 5 days, which corresponded to 0.47 g g-1 yield and productivity of 1.1 g L-1 h-1. Therefore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Erythritol; Glycerol; Span 20; Surfactants; Yarrowia lipolytica.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200417
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Evaluation of foam properties of saponin from Ilex paraguariensis A. St. Hil. (Aquifoliaceae) fruits BJPS
Canto,Gizele Scotti do; Treter,Janine; Yang,Samanta; Borré,Gustavo Luís; Peixoto,Maria Paula Garofo; Ortega,George González.
Saponins are natural compounds able to form abundant foam, a desirable quality required in some chemical, foods, cosmetic and pharmaceutical processes. Ilex paraguariensis A. St. Hil. (Aquifoliaceae) known as mate, is a South American widely cultivated specie due to the preparation of a tea-like beverage from its leaves. Moreover, its green fruits are a rich source of non-toxic and very low haemolytic saponins. In this study, mate saponin fraction (MSF) was evaluated as a foam former, focusing on its foamability, foam lifetime, and film drainage in the presence of different electrolytes (ionic strength I = 0.024 M). Sodium dodecyl sulphate (SDS) and polysorbate 80 (Poly-80) were used as reference surfactants. The critical micelle concentration (CMC), the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ilex paraguariensis; Aquifoliaceae; Mate; Saponins; Surfactants; Foam; Assay.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200010
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Germination and mycelial growth of Bipolaris euphorbiae Muchovej & Carvalho as influenced by herbicides and surfactants BJM
Nemoto,Marta Cecília Mallasen; Nahas,Ely; Pitelli,Robinson Antonio; Coelho,Lisias.
Bipolaris euphorbiae Muchovej & Carvalho can be combined with herbicide in order to control a large spectrum of weed species, being a strong candidate for the biocontrol of Euphorbia heterophylla L. (milk weed). The fungus release can be combined with herbicide in order to control a broader spectrum of weed species. Thus, laboratory experiments were set up to study the feasibility of using tank mixes of B. euphorbiae spores with herbicides or surfactants recommended for soybean. Mycelial growth and conidia germination were evaluated in PDA medium enriched with the herbicides oxasulfuron (80 g/ha), glyphosate (4 L/ha), bentazon (1.5 L/ha), fomesafen (1 L/ha), chlorimuron-ethyl (80 g/ha), lactofen (1 L/ha) and imazetaphyr (1 L/ha), and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolaris euphorbiae; Mycelial growth; Conidia germination; Herbicides; Surfactants.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400015
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Influence of Adjuvants on the Surface Tension, Deposition and Effectiveness of Herbicides on Fleabane Plants Planta Daninha
CASTRO,E.B.; CARBONARI,C.A.; VELINI,E.D.; GOMES,G.L.G.C.; BELAPART,D..
ABSTRACT: The use of herbicides is widespread in weed management, and adjuvants are added to the spray solution to improve the effectiveness of applications. The goal was to evaluate the influence of adjuvants added to the herbicides saflufenacil and glyphosate, isolated or in combination, and the effects on the surface tension, deposition and effectiveness in post-emergence applications to control Conyza sumatrensis. Experiments were conducted completely randomized experimental design to evaluate surface tension (TS), deposition and herbicide effectiveness. The treatments were glyphosate, glyphosate + saflufenacil and saflufenacil with or without the adjuvants Natur’al óleo®, Assist®, Aterbane®, Sillwet L-77 and Dash HC® (surface tension). For the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conyza sumatrensis; Glyphosate; Saflufenacil; Surfactants.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100267
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INTERACTIONS BETWEEN ADJUVANTS AND THE FUNGICIDE AZOXYSTROBIN+BENZOVINDIFLUPYR IN HYDRAULIC SPRAYING REA
Landim,Thiago N.; Cunha,João P. A. R. da; Alves,Guilherme S.; Marques,Matheus G.; Silva,Sérgio M..
ABSTRACT Adjuvants are tools to maximize pesticide spray quality. This study aimed to evaluate the interaction effects between adjuvants and the fungicide azoxystrobin + benzovindiflupyr on the physicochemical characteristics, droplet evaporation time, and spray droplet size. The experiment was carried out using a 2×6 factorial scheme, in which the first factor was the absence or presence of the fungicide mixture (water or water + fungicide) and the second factor was the presence of different adjuvants (water and five adjuvants). The parameters evaluated were surface tension, pH, viscosity, electrical conductivity, droplet evaporation time, volume median diameter, percentage of droplets smaller than 100 μm, and relative amplitude of the droplet spectrum....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spray solution additives; Droplet spectrum; Surfactants; Application technology.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162019000500600
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Lignolytic enzymes produced by Trametes villosa ccb176 under different culture conditions BJM
Yamanaka,Renata; Soares,Clarissa F.; Matheus,Dácio R.; Machado,Kátia M.G..
The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Metals; MnP; Vegetable oil; Surfactants.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100019
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Nanoemulsions as vehicles for transdermal delivery of glycyrrhizin BJPS
Harwansh,Ranjit Kumar; Patra,Kartik Chandra; Pareta,Surendra Kumar; Singh,Jagadish; Rahman,Mohammed Akhlaquer.
The present investigation aims to evaluate an isotropic and thermodynamically stable nanoemulsion formulation for transdermal delivery of glycyrrhizin (GZ), with minimum surfactant and cosurfactant (Smix) concentrations that could improve its solubility, permeation enhancement, and stability. Pseudo-ternary phase diagrams were developed and various nanoemulsion formulations were prepared using soyabean oil as oil, Span 80, Brij 35 as a surfactant and isopropyl alcohol as a cosurfactant. Nanoemulsion formulations that passed the thermodynamic stability tests were characterized for pH, viscosity and droplet size using a transmission electron microscopy. The transdermal ability of glycyrrhizin through human cadaver skin was determined using Franz diffusion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanoemulsions/use; Nanoemulsions/in vitro skin permeation; Glycyrrhizin/transdermal delivery; Isotropic nanoemulsion formulation/evaluation; Anti-inflammatories/transdermal delivery; Surfactants; Permeation/enhancers; Permeation/in vitro study.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400014
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Particle size and morphological characterization of cosmetic emulsified systems by Optical Coherence Tomography (OCT) BJPS
Prestes,Paula Souza; Peres,Daniela D'Almeida; Freitas,Anderson Zanardi de; Consiglieri,Vladi Olga; Kaneko,Telma Mary; Velasco,Maria Valéria Robles; Baby,André Rolim.
ABSTRACT The physicochemical attributes of emulsified systems are influenced by the characteristics of their internal phase droplets (concentration, size and morphology), which can be modified not only by the formulation components, but also by the analytical methodology employed. Thus, the aim of this work involved the physicochemical characterization of cosmetic emulsions obtained from different surfactants, as well as the introduction of the optical coherence tomography (OCT) as the analytical technique employed for the morphological characterization and particle size determination of the formulations. Three emulsions were prepared, differing at the type and concentration of the surfactant used, and their droplet sizes were evaluated through optical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cosmetic emulsions/physicochemical characterization; Surfactants; Optical coherence tomography/morphological characterization; Optical microscopy/morphological characterization; Laser diffraction/morphological characterization.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200273
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Produção de antígenos do vírus da artrite encefalite caprina para imunodiagnóstico. Repositório Alice
LOPES JUNIOR, C. A. F..
Resumo: Os Lentivírus de pequenos ruminantes são responsáveis por uma significante perda econômica que proporcionou o desenvolvimento de eficientes programas de controle e monitoramento na Europa e em outros países. O diagnóstico da CAE é baseado principalmente em testes sorológicos. A Organização Mundial da Saúde Animal (OIE) recomenda a utilização da Imunodifusão em gel de agarose (IDGA) pelo baixo custo, alta especificidade e praticidade de execução. Os antígenos utilizados para o diagnóstico sorológico são compostos principalmente pelas proteínas estruturais p28 e gp135. O IDGA pode determinar resultados falsonegativos, por ser um teste de baixa sensibilidade. Nesse sentido a produção de antígenos virais é importante nos testes de detecção da CAE,...
Tipo: Tese/dissertação (ALICE) Palavras-chave: CAEV; Surfactante; Imunodiagnóstico; Caprino; Doença animal; Lentivírus; Artrite encefalie caprina; Antígeno; Detergente; Retrovirus; Goats; Antigen Production; Detergent; Surfactants.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/928296
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Reporting the utilization and perspectives of different surface active agents for bread making Ciênc. Tecnol. Aliment.
ARSHAD,Nazish; AHMAD,Asif; NADEEM,Muhammad; AMIR,Rai Muhammad; ARSHAD,Muhammad Sajid.
Abstract The present study was planned to use surfactants in different concentrations along with varying amounts of shortening in the form of gels to prepare breads. For that purpose, SSL, CSL, PS-60 and GMS were used at level of 0.25, 0.50, 0.75 and 1.00 parts in combination with shortening @ 4.00, 3.75, 3.50 and 3.25 parts. The dough having surfactants gels resulted in longer resistance time and overall dough stability. The moisture and protein content of breads increased which is due to interactions of surfactant gels with starch and gluten fractions that result in increasing dough strength and baking quality. Significant increase in specific volume is observed in breads of GMS and PS-60 gels. The sensory evaluation was carried out by a panel of trained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breads; Surfactants; Rheology; Chemical analysis; Sensory evaluation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005020102
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Spreading of drops solutions with biological insecticide in different concentrations of adjuvants in cassava leaves Sci. Agrar.Paran. / SAP
Salvalaggio, Adriana Cologni; Costa, Neumárcio Vilanova da; Moratelli, Gustavo; Exteckoetter, Vanessa; Podkowa, Camila Inês; Cazzo, Victor Natan.
It was aimed to evaluate the efficiency of spreading drops in cassava leaves of Solutions with Biological insecticides and adjuvants in different concentrations. The experimental design was completely randomized in a factorial 2 x 3 x 5, with four replications. The treatments consisted in the preparation of solutions of two biological insecticide Bacillus thuringiensis (500 g ha- 1, Dipel WP® - 32,0 g kg-1) and baculovirus (0,41 g ha- 1) , with addition of three adjuvants (Mineral oil-Nimbus®, vegetable oil- Aureo® and glycerine- Hygrogem® , soybean biodiesel byproduct ) in five concentrations (0,0; 0,2; 0,4; 0,6 and 0,8 % v v- 1), cassava leaves comes the variety 'IAC 90'. The spreading of the drops (13μL) of the solutions were evaluated in adaxial and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Manihot esculenta; Surfactants; Application technology..
Ano: 2017 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/14183
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Sunglint images of current gradients at high resolution: Critical angle and directional observing strategy ArchiMer
Rascle, Nicolas; Nouguier, Frederic; Chapron, Bertrand; Ocampo-torres, Francisco J..
High-resolution satellite images of the ocean surface in and around the sunglint often provide unique observations of sub-mesoscale upper ocean surface processes. Local anomalies of wind, waves, currents or surfactants appear on the images as local anomalies of brightness. A quantitative interpretation of those brightness anomalies must relate them to slope properties of the wave field, which are to the lowest order described by the mean square slope (mss). The prevailing paradigm for such interpretation is that of the critical zenith angle. It states that, for sub-critical zenith view angle, brightness and mss anomalies have opposite signs, and this defines the so-called inversion region. This prevailing paradigm implicitly builds on the assumption that...
Tipo: Text Palavras-chave: Sea surface roughness; Surface current gradients; Submesoscale turbulence; Wave-current interaction; Surfactants; Sun glint imagery; SAR images; Multi-angle radiometers; Surface slope distribution; Mean square slope.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00448/55964/57498.pdf
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Toxicite aigue et effets subletaux, apres une courte exposition, de differents nonyphenols polyethoxyles sur la civelle. ArchiMer
Miossec, Laurence; Bocquene, Gilles.
The non-ionic surfactant group, polyethoxylated nonylphenols, show a toxicity to elvers in inverse relation to the number of ethylene oxide groups in the molecule. Their short-term effects on elver gills include much tissue disorganization and destruction.
Tipo: Text Palavras-chave: Molecular structure; Sublethal effects; Toxicity; Detergents; Surfactants.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/publication-1791.pdf
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Unique crystal structure of a novel surfactant protein from the foam nest of the frog Leptodactylus vastus. Repositório Alice
HISSA, D. C.; BEZERRA, G. A.; BIRNER-GRUENBERGER, R.; SILVA, L. P.; USÓN, I.; GRUBER, K.; MELO, V. M. M..
bitstream/item/115835/1/cavalcante2014.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Lv-ranaspumin; Structural biology; Amphibian; Mass spectrometry; Surfactants.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1005910
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Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides ArchiMer
Vandanjon, Laurent; Vallet, Laurent; Le Glatin, Titouan; Deleris, Paul; Baron, Regis; Bourseau, Patrick; Dumay, Justine.
The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.   After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial...
Tipo: Text Palavras-chave: Brown macroalgae; Sargassum muticum; Enzymatic hydrolysis; Surfactants; Phlorotannins.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00350/46105/46073.pdf
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