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A comparison of methods to determine tannin acyl hydrolase activity BABT
Aguilar,Cristóbal; Christopher,Augur; Viniegra-González,Gustavo; Favela,Ernesto.
Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tannin acyl hydrolase; Assay; Polyurethane foam; Solid state fermentation; Tannase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300014
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Characterization of a thermostable extracellular tannase produced under submerged fermentation by Aspergillus ochraceus Electron. J. Biotechnol.
Gonçalves,Heloísa Bressan; Riul,Alana Jacomini; Quiapim,Andréa Carla; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
Background: Tannases are enzymes that may be used in different industrial sectors as, for example, food and pharmaceutical. They are obtained mainly from microorganisms, as filamentous fungi. However, the diversity of fungi stays poorly explored for tannase production. In this article, Aspergillus ochraceus is presented as a new source of tannase with interesting features for biotechnological applications. Results: Extracellular tannase production was induced when the fungus was cultured in Khanna medium with tannic acid as carbon source. The extracellular tannase was purified 9-fold with 2% recovery and a single band corresponding to 85 kDa was observed in SDS-PAGE. The native apparent molecular mass was estimated as 112 kDa. Optima of temperature and pH...
Tipo: Journal article Palavras-chave: Aspergillus ochraceus; Gallic acid; Tannase; Tannic acid; Tannin acyl hydrolase.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500004
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Tannase production by Penicillium atramentosum KM under SSF and its applications in wine clarification and tea cream solubilization BJM
Selwal,Manjit K; Yadav,Anita; Selwal,Krishan K; Aggarwal,N.K; Gupta,Ranjan; Gautam,S. K.
Tannin acyl hydrolase commonly known as tannase is an industrially important enzyme having a wide range of applications, so there is always a scope for novel tannase with better characteristics. A newly isolated tannase-yielding fungal strain identified as Penicillium atramentosum KM was used for tannase production under solid-state fermentation (SSF) using different agro residues like amla (Phyllanthus emblica), ber (Zyzyphus mauritiana), jamun (Syzygium cumini), Jamoa (Eugenia cuspidate) and keekar (Acacia nilotica) leaves. Among these substrates, maximal extracellular tannase production i.e. 170.75 U/gds and 165.56 U/gds was obtained with jamun and keekar leaves respectively at 28ºC after 96 h. A substrate to distilled water ratio of 1:2 (w/v) was found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tannin acyl hydrolase; Agro residues; Penicillium atramentosum KM; Jamun leaves; SSF.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100047
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