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Registros recuperados: 15
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Approches culturales et moléculaires des assemblages microbiens associés aux polychètes hydrothermaux de la famille Alvinellidae ArchiMer
Alain, Karine.
Cultural and molecular approaches were used to analyse the microbial assemblages associated to the deep-sea hydrothermal vent polychaete worms of the family Alvinellidae. In a first part of the work, the populations associated to three alvinellid species from the Juan de Fuca Ridge (Paralvinella palmiformis, Paralvinella pandorae and Paralvinella sulfincola) were identified by phylogenetic analyses. A wide microbial diversity was detected, like a great number of new sequences or of sequences belonging to species previously unidentified at deep-sea hydrothermal vents. All the libraries were found to be dominated by sequences affiliated to the epsilon-Proteobacteria. In other respects, fermentative micro-organisms belonging to the Thermococcales, the...
Tipo: Text Palavras-chave: Paralvinella; Alvinella; 16S rRNA; Thermophile; Hydrothermal vent; Paralvinella; Alvinella; ARNr 16S; Thermophile; Source hydrothermale.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2005/these-332.pdf
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Caloranaerobacter azorensis gen. nov., sp nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent ArchiMer
Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The...
Tipo: Text Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf
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Caminicella sporogenes gen. nov., sp nov., a novel thermophilic spore-forming bacterium isolated from an East-Pacific Rise hydrothermal vent ArchiMer
Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne.
A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex...
Tipo: Text Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf
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Clostridium caminithermale sp nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney ArchiMer
Brisbarre, Nadège; Fardeau, Marie-laure; Cueff, Valerie; Cayol, Jean-luc; Barbier, Georges; Cilia, Valérie; Ravot, Gilles; Thomas, Pierre; Garcia, Jean-louis; Ollivier, Bernard.
A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3(T) was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3(T) possessed a few laterally inserted flagella, had a DNA G+C content of 33.1 mol% and grew optimally at pH 6.6 and at 45 degreesC. Growth was observed at temperatures between 20 and 58 degreesC and at pH values between 5.8 and 8.2. The optimum NaCl concentration for growth was 3% sea salt (30 g l(-1)); no growth was observed in the presence of 15 or 60 g sea salt l(-1). Strain DVird3(T) is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from...
Tipo: Text Palavras-chave: Phylogeny; Thermophile; Bacteria; Deep sea hydrothermal vent.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-588.pdf
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Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l BJM
Fawzi,Eman Mohamed.
Thirty fungal species grown on Cichorium intybus L. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. The thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 gave the highest production levels of inulinases I & II at 50 and 24 ºC respectively. Yeast extract and peptone were the best nitrogen sources for highest production of inulinases I & II at five and seven days of incubation respectively. The two inulinases I & II were purified to homogeneity by gel-filtration and ion-exchange chromatography with 66.0 and 42.0 fold of purification respectively. The optimum temperatures of purified inulinases I & II were 75 and 50 ºC respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inulinases; Production; Purification; Thermophile; Thermostability.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200028
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Complete genome sequence and whole-genome phylogeny of Kosmotoga pacifica type strain SLHLJ1T from an East Pacific hydrothermal sediment ArchiMer
Jiang, Lijing; L'Haridon, Stephane; Jebbar, Mohamed; Xu, Hongxiu; Alain, Karine; Shao, Zongze.
Kosmotoga pacifica strain SLHLJ1T is a thermophilic chemoorganoheterotrophic bacterium isolated from a deep-sea hydrothermal sediment. It belongs to the physiologically homogeneous Thermotogaceae family. Here, we describe the phenotypic features of K. pacifica together with its genome sequence and annotation. The chromosome has 2,169,170 bp, organized in one contig. A total of 1897 candidate protein-encoding genes and 177 RNA genes were identified. The 16S rRNA gene sequence of this strain is distantly related to sequences of some relatives classified in the same genus (K. olearia 7.02% and K. shengliensis 7.83%), with dissimilarity percentages close to the threshold generally described for genus delineation. Nevertheless, the percentage of conserved...
Tipo: Text Palavras-chave: Marine; Hydrothermal vent; Thermotogales; Chemoorganoheterotroph; Thermophile.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48191/48324.pdf
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Continuous enrichment culture and molecular monitoring to investigate the microbial diversity of thermophiles inhabiting deep-sea hydrothermal ecosystems ArchiMer
Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne.
The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture...
Tipo: Text Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf
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Deferribacter autotrophicus sp nov., an iron(III)-reducing bacterium from a deep-sea hydrothermal vent ArchiMer
Slobodkina, G. B.; Kolganova, T. V.; Chernyh, N. A.; Querellou, Joel; Bonch-osmolovskaya, E. A.; Slobodkin, A. I..
A thermophilic, anaerobic, chemolithoautotrophic bacterium (designated strain SL50(T)) was isolated from a hydrothermal sample collected at the Mid-Atlantic Ridge from the deepest of the known World ocean hydrothermal fields, Ashadze field (12 degrees 58' 21 '' N 44 degrees 51' 47 '' W) at a depth of 4100 m. Cells of strain SL50(T) were motile, straight to bent rods with one polar flagellum, 0.5-0.6 mu m in width and 3.0-3.5 mu m in length. The temperature range for growth was 25-75 degrees C, with an optimum at 60 degrees C. The pH range for growth was 5.0-7.5, with an optimum at pH 6.5. Growth of strain SL50(T) was observed at NaCl concentrations ranging from 1.0 to 6.0% (w/v) with an optimum at 2.5 % (w/v). The generation time under optimal growth...
Tipo: Text Palavras-chave: Thermophile; Archaeon.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6598.pdf
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Desulfurobacterium crinifex sp.nov., a novel thermophilic, pinkish-streamer forming, chemolithoautotrophic bacterium isolated from a Juan de Fuca Ridge hydrothermal vent and amendment of the genus Desulfurobacterium ArchiMer
Alain, Karine; Rolland, Sophie; Crassous, Philippe; Lesongeur, Francoise; Zbinden, Magali; Le Gall, Christian; Godfroy, Anne; Page, Antoine; Juniper, S Kim; Cambon-bonavita, Marie-anne; Duchiron, Francis; Querellou, Joel.
A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200...
Tipo: Text Palavras-chave: Thermophile; Nitrate reducing; Lithotroph; Desulfurobacterium crinifex; Deep sea hydrothermal vent; Cell networks.
Ano: 2003 URL: https://archimer.ifremer.fr/doc/2003/publication-663.pdf
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Exploration des communautés virales thermophiles dans les écosystèmes chauds des Terres australes et antarctiques françaises ArchiMer
Parikka, Kaarle Joonas.
Viruses thrive in all types of ecosystems where life is found. They represent the most abundant biological entity of our biosphere. Though several studies have been conducted on viral abundance and dynamics in mesophilic aquatic ecosystems, these aspects remain largely unexplored in extremophilic environments, such as hot springs. In this study, prokaryotic and associated viral communities of the French Southern and Antarctic Lands hot springs were explored. First, prokaryotic cells and Virus-like particles (VLP) were enumerated in several terrestrial and inshore hot springs. The results reveal an abundance of 105 - 106 particles/ml in both types of hot springs studied. The virus-to-prokaryote ratios (VPR) were generally low, confirming thus actual...
Tipo: Text Palavras-chave: Bactérie; Archée; Induction virale; Lysogénie; Source chaude; Abondance; Bacteria; Archaea; Phage; Thermophile; Viral induction; Lysogeny; Hot spring; Abundance.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00609/72139/70891.pdf
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Genomic Insights into the Carbon and Energy Metabolism of a Thermophilic Deep-Sea Bacterium Deferribacter autotrophicus Revealed New Metabolic Traits in the Phylum Deferribacteres ArchiMer
Slobodkin, Alexander; Slobodkina, Galina; Allioux, Maxime; Alain, Karine; Jebbar, Mohamed; Shadrin, Valerian; Kublanov, Ilya; Toshchakov, Stepan; Bonch-osmolovskaya, Elizaveta.
nformation on the biochemical pathways of carbon and energy metabolism in representatives of the deep lineage bacterial phylum Deferribacteres are scarce. Here, we report the results of the sequencing and analysis of the high-quality draft genome of the thermophilic chemolithoautotrophic anaerobe Deferribacter autotrophicus. Genomic data suggest that CO2 assimilation is carried out by recently proposed reversible tricarboxylic acid cycle (“roTCA cycle”). The predicted genomic ability of D. autotrophicus to grow due to the oxidation of carbon monoxide was experimentally proven. CO oxidation was coupled with the reduction of nitrate to ammonium. Utilization of CO most likely involves anaerobic [Ni, Fe]-containing CO dehydrogenase. This is the first evidence...
Tipo: Text Palavras-chave: Autotrophic; Thermophile; RoTCA cycle; CO oxidation; Fe(III)-reduction; Nitrate reduction.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00589/70078/68051.pdf
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Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent ArchiMer
Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf
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Marinitoga hydrogenitolerans sp nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge ArchiMer
Postec, Anne; Le Breton, Claire; Fardeau, Marie-laure; Lesongeur, Francoise; Pignet, Patricia; Querellou, Joel; Ollivier, Bernard; Godfroy, Anne.
A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100% hydrogen or in the presence of 2% oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is...
Tipo: Text Palavras-chave: Anaerobic bacterium; Marinitoga hydrogenitolerans; Thermotogales; Thermophile; Deep sea vent.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-363.pdf
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Thermodesulfobacterium hydrogeniphilum sp nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at Guaymas Basin, and emendation of the genus Thermodesulfobacterium ArchiMer
Jeanthon, Christian; L' Haridon, Ste! Phane; Cueff, Valerie; Banta, Amy; Reysenbach, Anna-louise; Prieur, Daniel.
A thermophillic, non-spore-forming, marine, sulfate-reducing bacterium, strain SL6(T), was isolated from deep-sea hydrothermal sulfides collected at Guaymas Basin. The Gram-negative-staining cells occurred singly or in pairs as small, highly motile rods. The temperature range for growth was 50-80degreesC with an optimum at 75degreesC. The pH range for growth at 70degreesC was 6.3-6.8, with an optimum at 6.5. The NaCl concentration range for growth was 5.55 g l(-1), with an optimum at 30 g l(-1). H-2 and CO2 were the only substrates for growth and sulfate reduction. However, growth was stimulated by several organic compounds. Sulfur, thiosulfate, sulfite, cystine, nitrate and fumarate were not used as electron acceptors. Pyruvate, lactate and malate did not...
Tipo: Text Palavras-chave: Sulfate reduction; Thermodesulfobacterium; Bacteria; Thermophile; Deep sea hydrothermal vent.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-585.pdf
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Thermosipho atlanticus sp nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent ArchiMer
Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges.
A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the...
Tipo: Text Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf
Registros recuperados: 15
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