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A study of cold tolerance in rice (Oryza sativa L.) International Rice Research Institute
Amirkolaei, Sayyed Kamal Kazemitabar.
x, 328 leaves : ill. Thesis (Ph.D.) -- University of Liverpool
Tipo: Thesis Palavras-chave: Rice; Tissue culture; Cold tolerance; Plant breeding.
Ano: 1997 URL: http://hdl.handle.net/123456789/589
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Absorcao de macronutrientes por explantes de bananeira in vitro. Repositório Alice
DINIZ, J. D. N.; GONÇALVES, A. N.; HERNANDEZ, F. F. F.; TORRES, A. C..
Com o objetivo de estudar a absorcao de macronutrientes (N, P, K, Ca, Mg e S) em explantes de bananeira cv. Prata ana, foram utilizados explantes de plantas estabelecidas in vitro, inoculados em meio basico de Murashing & Skoog (1962) contendo sacarose (30 g/L), e BAP (3,5 mg/L) com sete tratamentos, representados pelos periodos de 0, 10, 20, 30, 40, 50 e 60 dias de cultivo e tres repeticoes. As quantidades de macronutrientes totais absorvidas pelos explantes seguiram a ordem: K> N> Ca>ou= P>Mg =S. O P foi o nutriente absorvido mais rapidamente pelo explantes, com 75% extraido do meio de cultivo nos primeiros 30 dias, cessando sua absorcao aos 50 dias, restando ainda 9% no meio de cultivo. A absorcao do S cessou tambem aos 50 dias, quando...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Musa; Cultura de tecidos; Materia seca; Nutrientes; Tissue culture; Dry matter; Nutrients.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/84317
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Acclimatization of 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstock Scientia Agricola
Machado,Marília Pereira; Biasi,Luiz Antonio; Ritter,Marlice; Ribas,Luciana Lopes Fortes; Zanette,Flávio.
The pre-acclimatization stage can be used to improve micropropagation protocols and increase the yield of produced plants. The influence of sucrose and photon flux density (PFD) levels on the acclimatization of in vitro-grown 'VR043-43' (Vitis vinifera x Vitis rotundifolia) grapevine rootstocks was evaluated. Rooted shoots were obtained from 4-week-old in vitro shoots cultivated in QL (Quoirin and Lepoivre, 1977) culture medium supplemented with 15, 30 and 45 g L-1 of sucrose. The experiment was kept in a 25 ± 2ºC growth room, under 16-h photoperiod and PFD of 18 µmol m-2 s-1 or 43 µmol m-2 s-1. Plants were transferred to an intermittent misting system greenhouse for 10 d followed by 20 d of once-a-day watering routine using a handheld hose. Plant height...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue culture; Sucrose; Micropropagation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400007
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Acumulo de materia seca e exigencias nutricionais de plantas de alho provenientes de cultura de tecidos e de propagação convencional. Repositório Alice
RESENDE, F. V.; FAQUIN, V.; SOUZA, R. J. de; SANTOS, V. S..
1999
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alho; Cultura de tecido; Materia seca; Nutricao mineral; Multiplicacao in vitro; Allium sativum; Garlic; Tissue culture; Dry matter; Mineral nutrition; In vitro multiplication.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/766151
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Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes. Repositório Alice
ROESLER, E. A.; MANFROI, E.; MORAS, A.; DIDONÉ, D. A.; GRANDO, M. F.; LAU, E. Y..
bitstream/item/186497/1/ID44408-2018CienciRuralv48n11e20170806.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum Aestivum; Trigo; Cultura de Tecido; Regulador de Crescimento; Triticum; Wheat; Tissue culture; Growth regulators.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099731
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Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes. Repositório Alice
ROESLER, E. A.; MANFROI, E.; MORAS, A.; DIDONÉ, D. A.; GRANDO, M. F.; YAMAZAKI LAU, E..
bitstream/item/191594/1/ID44479-2018v48n11p1CienciaRural.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Triticum Aestivum; Trigo; Cultura de Tecido; Regulador de Crescimento; Wheat; Tissue culture; Growth regulators.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105050
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Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes Ciência Rural
Roesler,Eduardo André; Manfroi,Ernandes; Morás,Andréa; Didoné,Dielli Aparecida; Grando,Magali Ferrari; Lau,Elene Yamazaki.
ABSTRACT: The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Tissue culture; Growth regulators.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100402
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ADVANCES IN GUAVA PROPAGATION Rev. Bras. Frutic.
PEREIRA,FERNANDO MENDES PEREIRA; USMAN,MUHAMMAD; MAYER,NEWTON ALEX; NACHTIGAL,JAIR COSTA; MAPHANGA,OSCAR RANNY MBONGENI; WILLEMSE,SALOMIE.
ABSTRACT Guava (Psidium guajava L.) can be propagated by seed, layering, air layering, grafting (budding or grafting), cuttings (root or shoot) or tissue culture. Propagation by seed is used for rootstock production and for raising populations for screening at early phases in the breeding programs. Vegetative propagation methods are used to clone selected genotypes from these programs and commercial orchards because it perpetuates all characteristics of each cultivar. This review addresses different methods that can be used to propagate guava, the methods commercially adopted and the progress obtained in recent years. There are several propagation technologies available, however, the adoption levels are rather different between producing countries. Needs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psidium guajava; Seeds; Grafting; Cutting; Air layering; Tissue culture.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401002
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Advances in passion fruit (Passiflora spp.) propagation. Repositório Alice
FALEIRO, F. G.; JUNQUEIRA, N. T. V.; JUNGHANS, T. G.; JESUS, O. N. de; MIRANDA, D.; OTONI, W. C..
The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Maracujá; Germinação; Propagação Vegetativa; Estaca; Planta Porta-Enxerto; Cultura de Tecido; Seed germination; Vegetative propagation; Plant cuttings; Grafting (plants); Tissue culture.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114508
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Advances in passion fruit (Passiflora spp.) propagation Rev. Bras. Frutic.
Faleiro,Fábio Gelape; Junqueira,Nilton Tadeu Vilela; Junghans,Tatiana Góes; Jesus,Onildo Nunes de; Miranda,Diego; Otoni,Wagner Campos.
Abstract The propagation of many passion fruit species (Passiflora spp.) is done by seeds or sexual method, although asexual propagation, such stem cuttings, grafting or tissue culture is also possible and very useful in many cases. In this work, it was presented information on scientific and technological advances in sexual and asexual propagation of passion fruit, considering the different propagation methods and their applications. New demands for research and development activities are also related, given the wide range of passion fruit species and its potential for food, ornamental and medicinal use.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Seed germination; Vegetative propagation; Cuttings; Grafting; Tissue culture.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000201002
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Advances in peach, nectarine And plum propagation¹. Repositório Alice
MAYER, N. A.; BIANCHI, V. J.; FELDBERG.
bitstream/item/168505/1/Alex-Mayer-RBF-PEACH-Propagation-2017.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Prunus spp; Seeds; Cutting; Grafting; Air layering; Tissue culture.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1081949
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ADVANCES IN PEACH, NECTARINE AND PLUM PROPAGATION Rev. Bras. Frutic.
MAYER,NEWTON ALEX; BIANCHI,VALMOR JOÃO; FELDBERG,NELSON PIRES; MORINI,STEFANO.
ABSTRACT Nursery trees of stone fruits (Prunus spp.) are traditionally produced by union of two distinct genotypes - the rootstock and the scion - which, by grafting, form a composite plant that will be maintained throughout of all plant life. In Brazil, the rootstocks are predominantly seed propagated and therefore usually results in heterogeneous trees for vigor and edaphic adaptation. However, with advances in rootstock breeding programs that released cultivars and certification in several countries (notably in Europe), the system will come gradually evolving for vegetative propagation (cuttings and tissue culture) and use of seeds of selected rootstocks with specific characteristics and potted nursery trees production. For scion cultivar propagation,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prunus spp.; Seeds; Cutting; Grafting; Air layering; Tissue culture.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000401001
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Agrobacterium-mediated transformation of sorghum. Repositório Alice
CARVALHO, C.H.S.; ZEHR, U.B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H.; HODGES, T.K.; AXTELL, J.D..
2000
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Sorgo; Transformacao; Genetica; Cultura de tecidos; Sorghum bicolor; Agrobacterium; Genetic; Transformation; Tissue culture.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/484026
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Alterações anatômicas de bananeiras micropropagadas em resposta a aclimatização ex vitro. Repositório Alice
COSTA, F. H. da S.; CASTRO, E. M. de; PASQUAL, M.; PEREIRA, J. E. S.; OLIVEIRA, C. de.
bitstream/item/178633/1/SP-19862-ID-32291.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Alterações estruturais; Rustificação ex vitro; Structural alterations; Ex vitro hardening; Cultura de tecido; Musa; Tissue culture.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/660677
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Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome) Biol. Res.
CAVIEDES,PABLO; CAVIEDES,RAÚL; RAPOPORT,STANLEY I.
Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the trisomy 16 fetal mouse, an animal model of the human condition. Cultured trisomic DRG neurons from human and mouse fetuses present faster rates of depolarization and repolarization in the action potential compared to normal controls and a shorter spike duration. Also, trisomy 16 brain and spinal cord tissue exhibit reduced acetylcholine secretion. Therefore, we decided to study Ca2+ currents in cultured DRG neurons from trisomy 16 and age-matched control mice, using...
Tipo: Journal article Palavras-chave: Trisomy 16; Dorsal root ganglion; Calcium current; Tissue culture; Patch clamp; Down syndrome.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009
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An efficient protocol for culturing meristems of sorghum hybrids Phyton
Sadia¹,B; Josekutty,PC; Potlakayala,SD; Patel,P; Goldman,S; Rudrabhatla,SV.
A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype NC+262, followed by NC+6C21 (31.3 ± 0.8), and NC+6B50 (16 ± 1.7). MS medium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Sorghum bicolor; Tissue culture; Genetic improvement; Genotypes.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200010
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Anais... Infoteca-e
SIMPÓSIO NACIONAL DE CULTURA DE TECIDOS VEGETAIS 1., 1985, Brasília, DF..
1986
Tipo: Documentos (INFOTECA-E) Palavras-chave: Planta; Cultura de tecidos; Congresso; Brasil; Plants; Tissue culture; Congress; Brazil.
Ano: 1986 URL: http://www.infoteca.cnptia.embrapa.br/handle/doc/757840
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Anatomical studies of in vitro organogenesis induced in leaf-derived explants of passionfruit. Repositório Alice
GLORIA, B.A. da; VIEIRA, M.L.C.; DORNELAS, M.C..
With the aim os studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of paranchymal origin.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology; Cultura de tecidos; Micropropagacao; Histologia.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/101050
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ANATOMICAL STUDIES OF IN VITRO ORGANOGENESIS INDUCED IN LEAF-DERIVED EXPLANTS OF PASSIONFRUIT PAB
GLORIA,BEATRIZ APPEZZATO DA; VIEIRA,MARIA LUCIA CARNEIRO; DORNELAS,MARCELO CARNIER.
With the aim of studying the organogenesis in vitro in Passiflora edulis Sims f. flavicarpa Deg., the passionfruit, leaf-derived explants were cultured on media containing NAA or BAP and incubated either in continuous darkness or in light. The histological events leading to de novo organ formation were evaluated. Darkness induces rhizogenesis in the presence of NAA, whereas direct shoot regeneration is stimulated by light and BAP. This latter condition is recommended for passionfruit micropropagation as several adventitious shoot buds were formed from meristemoids of parenchymal origin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Tissue culture; Micropropagation; Histology.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001100005
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Anatomical study of Racosperma (ex-Acacia) mangium tissues cultured in vitro BABT
Takemori,N. K.; Marschner,R.; Quoirin,M.; Bona,C.; Zanette,F..
In order to achieve adventitious shoot regeneration from young tissues of Racosperma mangium, several combinations of growth regulators were tested. Kinetin (KIN) and a -naphthalenacetic acid (NAA), thidiazuron (TDZ) and NAA or indole-3-butyric acid (IBA), 4-CPPU and 2,4-dichlorophenoxyacetic acid (2,4-D) were added to basic medium composed of MS salts, MS vitamins and 3% sucrose. Sections of hypocotyls and cotyledons of young seedlings were cultivated on these media. Calli developed on different media and, after two to three months, some of them regenerated organized structures. Histological studies revealed hypertrophy and hyperplasy of medular and cortical parenchyma cells of hypocotyl and cotyledon parenchyma. Formation of meristematic cells and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 4-CPPU; Leguminosae; TDZ; Tissue culture.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100008
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