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Registros recuperados: 8
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Effect of fungicide on Fusarium verticillioidesmycelial morphology and fumonisin B1 production BJM
Miguel,Tatiana de Á.; Bordini,Jaqueline G.; Saito,Gervásio H.; Andrade,Célia G.T. de J.; Ono,Mario A.; Hirooka,Elisa Y.; Vizoni,Édio; Ono,Elisabete Y.S..
The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that the fungicide caused inhibition of hyphal growth and defects on hyphae morphology such as cell wall disruption, withered hyphae, and excessive septation. In addition, extracellular material around the hyphae was rarely observed in the presence of fludioxonil + metalaxyl-M. While promoting the reduction of mycelial growth, the fungicide increased sporulation of F. verticillioides compared to the control, and the highest production occurred on the 14th day in the treatments and on the 10th day in the control cultures. Fumonisin B1production in the culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Mycotoxin; Scanning electron microscopy; Electron micrographs; Extracellular material.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100293
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Fumonisins in corn: correlation with Fusarium sp. count, damaged kernels, protein and lipid content BABT
Ono,Elisabete Yurie Sataque; Biazon,Luciana; Silva,Marcelo da; Vizoni,Édio; Sugiura,Yoshitsugu; Ueno,Yoshio; Hirooka,Elisa Yoko.
Natural fungal and fumonisin contamination were evaluated in 109 freshly harvested corn samples from Paraná State and correlated to damaged kernels (%). In addition, healthy and damaged kernels of 24 corn samples were selected in order to compare the mycoflora profile and fumonisin levels. The correlation among protein/lipid content and fumonisin levels was also analyzed in the 15 most frequently cultivated corn hybrids. Total fungal colony count in 109 freshly harvested corn samples ranged from 1.9x10(4) to 3.5x10(6) CFU/g, Fusarium sp. count from 1.0x10³ to 2.2x10(6) CFU/g, and fumonisin levels from 0.13 to 20.38 µg/g. Total fungal colony/Fusarium sp. count and fumonisin levels showed positive correlation (p < 0.05). In addition, there was a positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoflora; Toxigenic fungi; Mycotoxins; Fumonisins; Corn quality.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100008
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Genetic relationships among strains of the Aspergillus niger aggregate BABT
Ferracin,Lara Munique; Frisvad,Jens Christian; Taniwaki,Marta Hiromi; Iamanaka,Beatriz Thie; Sartori,Daniele; Schapovaloff,Maria Elena; Fungaro,Maria Helena Pelegrinelli.
We analyzed the genetic relationships between 51 fungal isolates previously identified as A. niger aggregate, obtained from dried fruit samples from worldwide origin and 7 A. tubingensis obtained from Brazilian coffee beans samples. Greater fungal diversity was found in black sultanas. Aspergillus niger sensu stricto was the most prevalent species. It was found in all fruit substrates of all geographical origins. Based on Random Amplification of Polymorphic DNA (RAPD) and β-tubulin sequences data two groups of A. niger were found. In spite of the small number of isolates from Group IV an association between extrolite patterns and molecular clustering is speculated. A. tubingensis were the second most frequent species and this species were clearly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger aggregate; Ochratoxin A; Dried fruits; Toxigenic fungi.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700031
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Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach Ciência Rural
Silva,Janaina Lara da; Aparecido,Christiane Ceriani; Hansen,Daiane; Pereira,Tacila Alves Muniz; Felicio,Joana D'arc; Gonçalez,Edlayne.
Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Aflatoxin; Cyclopiazonic acid; Feed; Molecular characterization..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
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Incidence and distribution of filamentous fungi during fermentation, drying and storage of coffee (Coffea arabica L.) beans BJM
Silva,Cristina Ferreira; Batista,Luis Roberto; Schwan,Rosane Freitas.
The objective of this work was to isolate and characterize filamentous fungi present in different stages of harvest, fermentation, drying and storage of coffee beans processed by natural method. The cherries were hand-picked and then placed on a cement drying platform where they remained until reached 11% of humidity. Microbial counts were found in all samples during fermentation and drying of the coffee beans. Counts of fungi in the coffee cherries collected from the tree (time 0) were around 1.5 x 10³ CFU/g. This number increased slowly during the fermentation and drying reaching values of 2 x 10(5) CFU/g within 22 days of processing. Two hundred and sixty three isolates of filamentous fungi were identified. The distribution of species during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Filamentous fungi; Coffee; Toxigenic fungi.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300022
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Methods to preserve potentially toxigenic fungi BJM
Guimarães,Lucas Costa; Fernandes,Ana Paula; Chalfoun,Sara Maria; Batista,Luís Roberto.
Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained characteristic is of paramount importance, so the process does not need to be repeated. Fungi are eukaryotic, achlorophyllous, heterotrophic organisms, usually filamentous, absorb their food, can be either macro or microscopic, propagate themselves by means of spores and store glycogen as a source of storage. Fungi, while infesting food, may produce toxic substances such as mycotoxins. The great genetic diversity of the Kingdom Fungi renders the preservation of fungal cultures for many years relevant. Several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxins; Toxigenic fungi; Methods for fungal conservation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100007
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Toxigenic fungi in beans (Phaseolus vulgaris L.) classes black and color cultivated in the State of Santa Catarina, Brazil BJM
Costa,Léa Luzia Freitas; Scussel,Vildes Maria.
Toxigenic fungi were studied in beans (Phaseolus vulgaris L.) of Classes black and color, cultivated in different regions of the State of Santa Catarina, south region of Brazil. The mean counts of filamentous fungi were 2.8 x 103 and 6.7 x 103 CFU/g for beans Classes black and color, respectively. Penicillium spp., Aspergillus spp. and Phoma spp. were the most frequent genera isolated, followed by Ryzopus spp., Alternaria spp., Helminthosporium spp., Cladosporium spp., Botrytis spp., Fusarium spp., Trichoderma spp., Curvularia spp. and Dreschelera spp. Among beans Class black, 24.6% of the Aspergillus strains produced mycotoxins: 13.1% produced aflatoxins (AFs); 11.5% produced ochratoxin A (OTA) and 28.9% of Penicillium produced citrinin (CTR). On the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beans; Phaseolus; Toxigenic fungi; Aflatoxin; Ochratoxin A; Citrinin.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200008
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Toxigenic mycobiota and mycotoxins in shrimp feed Ciência Rural
Calvet,Rodrigo Maciel; Pereira,Maria Marlúcia Gomes; Costa,Amilton Paulo Raposo; Torres,Adriana Mabel; Muratori,Maria Christina Sanches.
The objective of this study was to identify the toxigenic mycobiota and the occurrence of aflatoxins in shrimp feed products intended for shrimp cultivated in the coastal area of the state of Piauí, Brazil, in three farms ("A", "B" and "C"). The toxigenic capacity of the fungal species isolated was tested for aflatoxins (AF) and ochratoxin A production. The fungal counts of shrimp feed were similar for the "A" and "B" farms at all cultivation phases, collection sites, in closed and opened packages (1.33 to 2.66CFU g-1 log10 -1). The lowest fungal counts were found in feed from "C" farm (0.65CFU g-1 log10 -1) from closed packages. Thirty-four strains of Aspergillus were detected with a greater prevalence of A. flavus. Two strains produced B1, B2, G1 and G2...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Feed storage; Toxigenic fungi; Mycotoxins; Aflatoxin B1.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601021
Registros recuperados: 8
Primeira ... 1 ... Última
 

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