Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 13
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol.
Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi.
Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other...
Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014
Imagem não selecionada

Imprime registro no formato completo
Agronomic evaluation of genetically modified soybean genotypes in response to water deficit. Repositório Alice
LIMA, L. F. S. de; FERREIRA, L. C.; FUGANTI-PAGLIARINI, R.; MARIN, S. R. R.; OLIVEIRA, M. C. N. de; MERTZ-HENNING, L. M.; FARIAS, J. R. B.; NEUMAIER, N.; NAKASHIMA, K.; NUNES, L. M.; NEPOMUCENO, A. L..
bitstream/item/188954/1/Lima-77.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Resistência a Seca; Soja; Fator de Crescimento; Soybeans; Drought tolerance; Transcription factors; Plant growth.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102092
Imagem não selecionada

Imprime registro no formato completo
An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
Imagem não selecionada

Imprime registro no formato completo
AtDREB2A-CA influences root architecture and increases drought tolerance in transgenic cotton. Repositório Alice
LISEI-DE-SÁ, M. E.; ARRAES, F. B. N.; BRITO, G. G.; BENEVENTI, M. A.; LOURENCO-TESSUTTI, I.; BASSO, A. M. M.; AMORIM, R. M. S.; SILVA, M. C. M.; FAHEEM, M.; OLIVEIRA, N. G.; MIZOI, J.; YAMAGUCHI-SHINOZAKI, K.; GROSSI-DE-SA, M. F..
bitstream/item/167271/1/AS-2017103015574695.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Dehydration Responsive Element Binding Factors; Water Deficit Tolerance; Physiological Phenotyping; Stress-Inducible Promoter; Gossypium hirsutum; Transcription factors.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080442
Imagem não selecionada

Imprime registro no formato completo
Candidate genes and pathogenesis investigation for sepsis-related acute respiratory distress syndrome based on gene expression profile Biol. Res.
Wang,Min; Yan,Jingjun; He,Xingxing; Zhong,Qiang; Zhan,Chengye; Li,Shusheng.
BACKGROUND: Acute respiratory distress syndrome (ARDS) is a potentially devastating form of acute inflammatory lung injury as well as a major cause of acute respiratory failure. Although researchers have made significant progresses in elucidating the pathophysiology of this complex syndrome over the years, the absence of a universal detail disease mechanism up until now has led to a series of practical problems for a definitive treatment. This study aimed to predict some genes or pathways associated with sepsis-related ARDS based on a public microarray dataset and to further explore the molecular mechanism of ARDS RESULTS: A total of 122 up-regulated DEGs and 91 down-regulated differentially expressed genes (DEGs) were obtained. The up- and down-regulated...
Tipo: Journal article Palavras-chave: Acute respiratory distress syndrome; Sepsis; Differentially expressed mRNAs; Functional enrichment analysis; Pathway analysis; Transcription factors.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100025
Imagem não selecionada

Imprime registro no formato completo
Differential impact of acclimation and acute water deprivation in the expression of key transcription factors in soybean roots. Repositório Alice
GUIMARÃES-DIAS, F.; NEVES-BORGES, A. C.; CONFORTE, A. J.; GIOVANELLA-KAMPMANN, L.; FERREIRA, A. V. J.; AMORIM, R. M. S.; BENEVENT, M. A.; SÁ, M. E. L. de; MESQUITA, R. O.; RODRIGUES, F. A.; NEPOMUCENO, A. L.; ROMANO, E.; LOUREIRO, M. E.; GROSSI-DE-SA, M. F.; ALVES-FERREIRA, M..
bitstream/item/180696/1/Guimaraes-Dias2016-Article-DifferentialImpactOfAcclimatio.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Drought adaptation; Marker assistance selection; Water deficit; Glycine Max; Transcription factors.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056209
Imagem não selecionada

Imprime registro no formato completo
DREB1A allows for more water uptake in groundnut by a large modification in the root/shoot ratio under water deficit Open Agri
Vadez, V..
Palavras-chave: Transgenics; Evapotranspiration; Drought stress; Genotypes; Nutrient uptake; Transgenic plants; Transpiration (animals); Harvest index; Transcription; Transcription factors.
Ano: 2007 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3355
Imagem não selecionada

Imprime registro no formato completo
Overall picture of expressed Heat Shock Factors in Glycine max, Lotus japonicus and Medicago truncatula. Repositório Alice
SOARES-CAVALCANTI, N. M.; BELARMINO, L. C.; KIDO, E. A.; PANDOLFI, V.; MARCELINO-GUIMARÃES, F. C.; RODRIGUES, F. A.; PEREIRA, G. A. G.; BENKO-ISEPPON, A. M..
Heat shock (HS) leads to the activation of molecular mechanisms, known as HS-response, that prevent damage and enhance survival under stress. Plants have a flexible and specialized network of Heat Shock Factors (HSFs), which are transcription factors that induce the expression of heat shock proteins. The present work aimed to identify and characterize the Glycine max HSF repertory in the Soybean Genome Project (GENOSOJA platform), comparing them with other legumes (Medicago truncatula and Lotus japonicus) in view of current knowledge of Arabidopsis thaliana. The HSF characterization in leguminous plants led to the identification of 25, 19 and 21 candidate ESTs in soybean, Lotus and Medicago, respectively. A search in the SuperSAGE libraries revealed 68...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bioinformática; Soja; Gene; Genoma; Bioinformatics; Soybeans; Genome; Genes; Abiotic stress; Transcription factors.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/926601
Imagem não selecionada

Imprime registro no formato completo
Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells Biol. Res.
JAIMOVICH,ENRIQUE; ESPINOSA,ALEJANDRA.
We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The...
Tipo: Journal article Palavras-chave: Skeletal; Transcription factors; Insulin; IGF-I; Testosterone.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018
Imagem não selecionada

Imprime registro no formato completo
The use of sunflower transcription factors as biotechnological tools to improve yield and stress tolerance in crops Phyton
Chan,R.L.
Transcription factors (TFs) are proteins able to specifically recognize DNA sequences in the regulatory regions of their target genes. They bind these specific sequences, an event that leads to the activation or repression of whole signal transduction pathways. In plants about 1500 TFs were informatically identified; identification was mainly based in the presence of DNA-binding domains in the translated sequences. They were classified in families and subfamilies according to several features, including the conservation of the DNA binding domain, the genes structures and the functions they exert. Among transcription factors, several seem to be potential powerful biotechnological tools to improve crops via obtaining transgenic plants. Assigned purposes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sunflower; Transcription factors; HD-Zip; Homeodomain; Leucine zipper; Abiotic stress; Biotic stress.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100002
Imagem não selecionada

Imprime registro no formato completo
Transcription factors in microalgae: genome-wide prediction and comparative analysis ArchiMer
Thiriet-rupert, Stanislas; Carrier, Gregory; Chenais, Benoit; Trottier, Camille; Bougaran, Gael; Cadoret, Jean-paul; Schoefs, Benoit; Saint-jean, Bruno.
Background: Studying transcription factors, which are some of the key players in gene expression, is of outstanding interest for the investigation of the evolutionary history of organisms through lineage-specific features. In this study we performed the first genome-wide TF identification and comparison between haptophytes and other algal lineages. Results: For TF identification and classification, we created a comprehensive pipeline using a combination of BLAST, HMMER and InterProScan software. The accuracy evaluation of the pipeline shows its applicability for every alga, plant and cyanobacterium, with very good PPV and sensitivity. This pipeline allowed us to identify and classified the transcription factor complement of the three haptophytes...
Tipo: Text Palavras-chave: Algae; Endosymbiotic gene transfer; Haptophytes; Prediction pipeline; Stramenopiles; Tisochrysis lutea; Transcription factors.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00333/44403/44072.pdf
Imagem não selecionada

Imprime registro no formato completo
Transcritos de genes de defensa acumulados en chile CM334 infectado por Nacobbus aberrans o por Meloidogyne enterolobii e inoculado con Phytophthora capsici. Colegio de Postgraduados
Villar Luna, Edgar.
El chile CM334 es resistente al oomiceto Phytophthora capsici (Pc) y a los nematodos Meloidogyne incognita, M. arenaria y M. javanica, pero susceptible a M. enterolobii (Me) y Nacobbus aberrans (Na). La resistencia de Capsicum annuum a Pc se asocia con la acumulación de la fitoalexina sesquiterpenica capsidiol y con la sobre-expresión de varios genes de defensa. Los objetivos de este trabajo fueron: 1) conocer las modificaciones que Na y Me inducen en la expresión de algunos genes de la ruta mevalónica (HMG2, EAS, HMG3, y SS) en plantas CM334 infectadas por Na (NaPc) o Me (MPc) e inoculadas con Pc (Pc); 2) determinar la relación entre los niveles de HMG2 y EAS en raíces de CM334 inoculadas sólo con Na (N), con Pc (Pc) y con ambos patógenos (NPc); y 3)...
Palavras-chave: Capsicum annuum; Nematodos agalladores; QRT-PCR; Factores de transcripción; Ruta mevalónica; Root-knot nematodes; Transcription factors; Mevalonate pathway; Fitopatología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2113
Imagem não selecionada

Imprime registro no formato completo
Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. Repositório Alice
OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A..
A eficiência alimentar é uma característica moderadamente herdável, que por possuir uma mensuração onerosa e tardia, tem sua incorporação em programas de melhoramento animal dificultada. Estudos prévios de mapeamento identificaram o gene KCNJ7 7, que possui importante função sobre vias metabólicas relacionadas a eficiência alimentar, tais como a via de insulina, em uma região de QTL (quantitative trait loci) para maciez da carne.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sítios-alvo de fator de transcrição; Gado Nelore; Melhoramento Genético Animal; Bos Indicus; Animal breeding; Transcription factors.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095987
Registros recuperados: 13
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional