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Registros recuperados: 18
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A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus ArchiMer
De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud.
A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment...
Tipo: Text Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches. Repositório Alice
DINIZ, M. R. V.; PAIVA, A. L. B.; GUERRA-DUARTE, C.; NISHIYAMA JÚNIOR, M. Y.; MUDADU, M. de A.; OLIVEIRA, U. de; BORGES, M. H.; YATES, J. R.; JUNQUEIRA-DE-AZEVEDO, I. de.
Abstract. Phoneutria nigriventer is one of the largest existing true spiders and one of the few considered medically relevant. Its venom contains several neurotoxic peptides that act on different ion channels and chemical receptors of vertebrates and invertebrates. Some of these venom toxins have been shown as promising models for pharmaceutical or biotechnological use. However, the large diversity and the predominance of low molecular weight toxins in this venom have hampered the identification and deep investigation of the less abundant toxins and the proteins with high molecular weight. Here, we combined conventional and next-generation cDNA sequencing with Multidimensional Protein Identification Technology (MudPIT), to obtain an in-depth panorama of...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Proteômica; Transcriptoma; Phoneutria nigriventer; Proteomics; Transcriptomics.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102272
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Analysis of the genomic basis of functional diversity in dinoflagellates using a transcriptome-based sequence similarity network ArchiMer
Meng, Arnaud; Corre, Erwan; Probert, Ian; Gutierrez-rodriguez, Andres; Siano, Raffaele; Annamale, Anita; Alberti, Adriana; Da Silva, Corinne; Wincker, Patrick; Le Crom, Stephane; Not, Fabrice; Bittner, Lucie.
Dinoflagellates are one of the most abundant and functionally diverse groups of eukaryotes. Despite an overall scarcity of genomic information for dinoflagellates, constantly emerging high-throughput sequencing resources can be used to characterize and compare these organisms. We assembled de novo and processed 46 dinoflagellate transcriptomes and used a sequence similarity network (SSN) to compare the underlying genomic basis of functional features within the group. This approach constitutes the most comprehensive picture to date of the genomic potential of dinoflagellates. A core-predicted proteome composed of 252 connected components (CCs) of putative conserved protein domains (pCDs) was identified. Of these, 206 were novel and 16 lacked any functional...
Tipo: Text Palavras-chave: Genomics; Proteomics; Microbial biology; Molecular evolution; Protists; Transcriptomics.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00444/55550/57209.pdf
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Bioinformatics for the Citrus EST Project (CitEST) Genet. Mol. Biol.
Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A..
In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031
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csrB Gene Duplication Drives the Evolution of Redundant Regulatory Pathways Controlling Expression of the Major Toxic Secreted Metalloproteases in Vibrio tasmaniensis LGP32 ArchiMer
Nguyen, An Ngoc; Disconzi, Elena; Charriere, Guillaume; Destoumieux-garzon, Delphine; Bouloc, Philippe; Le Roux, Frederique; Jacq, Annick.
CsrBs are bacterial highly conserved and multiple-copy noncoding small RNAs (sRNAs) that play major roles in cell physiology and virulence. In the Vibrio genus, they are known to be regulated by the two-component system VarSNarA. They modulate the well-characterized quorum sensing pathway controlling virulence and luminescence in Vibrio cholerae and Vibrio harveyi, respectively. Remarkably, Vibrio tasmaniensis LGP32, an oyster pathogen that belongs to the Splendidus Glade, was found to have four copies of csrB, named csrB1-4, compared to two to three copies in other Vibrio species. Here, we show that the extra csrB4 copy results from a csrB3 gene duplication, a characteristic of the Splendidus Glade. Interestingly, csrB genes are regulated in different...
Tipo: Text Palavras-chave: Bacterial gene regulation; Bacterial sRNAs; Transcriptomics; Vibrio pathogenic to oysters; Host-pathogen interactions.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00476/58785/61319.pdf
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de novo assembly and transcriptome analysis of sugarcane leaves from contrasting varieties submited to prolonged water stress. Repositório Alice
BELESINI, A. A.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. da S.; CARVALHO, F. M. de S.; CARLIN, S. R.; CAZETTA, J. O.; FERRO, M. I. T.; PINHEIRO, D. G..
Sugarcane is an important crop, major source of sugar and alcohol, accounting for two-thirds of the world's sugar production. In Brazil, the sugarcane culture has expanded to areas with prolonged drought seasons, which is constraining its production. In order to identify genes and molecular process related to sugarcane drought tolerance, we performed de novo assembly and transcriptome analysis of two sugarcane genotypes, one tolerant and other sensitive to water stress, submitted to three water deficit condition (30, 60 and 90 days). The de novo assembly of leaves transcriptome was performed using short reads from Illumina RNA-Seq platform, which produced more than 1 billion reads, which were assembled into 177,509 and 185,153 transcripts sequences for the...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bioinformática; Cana-de-açúcar; Tolerância à seca; Transcriptoma; Bioinformatics; Sugarcane; Drought tolerance; Water stress; Transcriptomics.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1038946
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Déterminisme nutritionnel et génétique de la teneur en lipides musculaires chez la truite arc-en-ciel (Oncorhynchus mykiss) [Texte imprimé] : étude par analyse de l'expression de gènes candidats, du protéome et du transcriptome du foie et du muscle ArchiMer
Kolditz, Catherine-inès.
The objective of the study was to identify genes and proteins that are involved in the control of muscle fat deposition in rainbow trout. We analyzed the combined effects exerted by genetic selection and dietary treatment, which are the two main factors that can be used to manage body fat content. Two lines of rainbow trout, obtained after 3 generations of divergent selection for high or low muscle fat content, were fed diets containing either 10% or 23% lipids (% dry matter), for six months. We analyzed the activity and gene expression of key enzymes involved in energy utilization, and performed a more global approach through transcriptome (nylon microarray) and proteome (twodimensional electrophoresis) analysis. We analyzed the liver, which is the centre...
Tipo: Text Palavras-chave: Proteomics; Transcriptomics; Selective breeding; Dietary energy; Rainbow trout; Protéome; Transcriptome; Sélection génétique; Apport énergétique alimentaire; Truite arc en ciel.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6794.pdf
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Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus ArchiMer
Wellenreuther, Maren; Le Luyer, Jeremy; Cook, Denham; Ritchie, Peter A.; Bernatchez, Louis.
Identifying genes and pathways involved in domestication is critical to understand how species change in response to human-induced selection pressures, such as increased temperatures. Given the profound influence of temperature on fish metabolism and organismal performance, a comparison of how temperature affects wild and domestic strains of snapper is an important question to address. We experimentally manipulated temperature conditions for F1-hatchery and wild Australasian snapper (Chrysophrys auratus) for 18 days to mimic seasonal extremes and measured differences in growth, white muscle RNA transcription and hematological parameters. Over 2.2 Gb paired-end reads were assembled de novo for a total set of 33,017 transcripts (N50 = 2,804). We found...
Tipo: Text Palavras-chave: Domestication; Temperature; Transcriptomics; Growth; Sparidae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00476/58788/61321.pdf
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Evaluation of de novo RNA-Seq assemblers in differential expression experiments. Repositório Alice
CARVALHO, L. M.; DIA, Z.; SILVA, F. R. da.
The objective of this study is to evaluate methodologies applied on de novo transcriptomics studies, proposing criteria for ranking the data, in order to maximize the chance of correctly identifying a differentially expressed transcript.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Bioinformática; Genoma; Genome; Transcriptomics; Bioinformatics.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1035044
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Gene expression: a review on methods for the study of defense-related gene differential expression in plants. Repositório Alice
CASASSOLA, A.; BRAMMER, S. P.; CHAVES, M. S.; MARTINELLI, J. A.; GRANDO, M. F.; DENARDIN, N. D..
2013
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transcriptomics; CDNA; Metabolic Routes; Plant Defense; Gene Expression; Molecular Biology.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/981342
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Global liver gene expression differences in Nelore steers with divergent residual feed intake phenotypes. Repositório Alice
TIZIOTO, P.; COUTINHO, L. L.; DECKER, J. E.; SCHNABEL, R. D.; ROSA, C. O.; OLIVEIRA, P. S. N.; SOUZA, M. M.; MOURÃO, G. B.; TULLIO, R. R.; CHAVES, A. S.; LANNA, D. P. D.; ZERLOTINI NETO, A.; MUDADU, M. A.; TAYLOR, J. F.; REGITANO, L. C. A..
Background: Efficiency of feed utilization is important for animal production because it can reduce greenhouse gas emissions and improve industry profitability. However, the genetic basis of feed utilization in livestock remains poorly understood. Recent developments in molecular genetics, such as platforms for genome-wide genotyping and sequencing, provide an opportunity to identify genes and pathways that influence production traits. It is known that transcriptional networks influence feed efficiency-related traits such as growth and energy balance. This study sought to identify differentially expressed genes in animals genetically divergent for Residual Feed Intake (RFI), using RNA sequencing methodology (RNA-seq) to obtain information from genome-wide...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transcriptoma; Sequenciamento genético; Bioinformática; Bos indicus; RFI; Feed efficiency; Zebu; Feed conversion; Transcriptomics; Bioinformatics.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1034697
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Inter and intra-specific transcriptional and phenotypic responses of Pseudo-nitzschia under different nutrient conditions ArchiMer
Lema, Anais Kimberley; Metegnier, Gabriel; Quéré, Julien; Latimier, Marie; Youenou, Agnes; Lambert, Christophe; Fauchot, Juliette; Le Gac, Mickael.
Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a...
Tipo: Text Palavras-chave: Phenotype; Transcriptomics; Pseudo-nitzschia; Divergence; Nutrients.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00482/59392/62157.pdf
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Low-Molecular-Weight Fucoidan Induces Endothelial Cell Migration via the PI3K/AKT Pathway and Modulates the Transcription of Genes Involved in Angiogenesis ArchiMer
Bouvard, Claire; Galy-fauroux, Isabelle; Grelac, Francoise; Carpentier, Wassila; Lokajczyk, Anna; Gandrille, Sophie; Colliec-jouault, Sylvia; Fischer, Anne-marie; Helley, Dominique.
Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were...
Tipo: Text Palavras-chave: Fucoidan; Angiogenesis; Vasculogenesis; Migration; Signaling; Transcriptomics.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00308/41913/41187.pdf
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Spinning gland transcriptomics from two main Clades of Spiders (Order: Araneae) - insights on their molecular, anatomical and behavioral evolution. Repositório Alice
PROSDOCIMI, P; BITTENCOURT, D.; SILVA, F. R. da; KIRST, M.; MOTA, P. C.; RECH, E. L..
Characterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sequencia de DNA; Sequenciamento; Aranhas; Sequence analysis; Araneae; Transcriptomics.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/902190
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Transcriptome analysis in leaves, flowers and initial fruit development of Coffea arabica L. Repositório Alice
IVAMOTO, S. T.; REIS JUNIOR, O.; SAKURAY, L. M.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; PEREIRA, L. F. P..
2015
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Coffea arabica; Café arábica; Coffea arabica L; Transcriptomics; Fruiting.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1040107
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Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation ArchiMer
Zarski, Daniel; Thaovi Nguyen,; Le Cam, Aurelie; Montfort, Jerome; Dutto, Gilbert; Vidal, Marie-odile; Fauvel, Christian; Bobe, Julien.
Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were...
Tipo: Text Palavras-chave: Microarray; Genomics; Transcriptomics; Aquaculture; Controlled reproduction.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48220/48345.pdf
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Using a bespoke 'Omics' approach to devise a flexible new way to control Fusarium in wheat. Repositório Alice
KING, R.; URBAN, M.; BROWN, N.; MACHADO, A. K. F. M.; LEE, W. S.; KANYUKA, K.; SPARKS, C.; WEST, J.; YAMAZAKI-LAU, E.; TIBOLA, C. S.; LIMA, M. I. P. M.; TOGAWA, R. C.; MARTINS, N. F.; ARAGAO, F. J. L.; NICOLLI, C. P.; DEL PONTE, E. M.; TESSMANN, D. J.; FERNANDES, J. M. C.; HAMMOND-KOSACK, K..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Trigo; Doença; Genome wide analyses; Transcriptomics; Fusarium venenatum; Fusarium culmorum; Host induced gene silencing; Wheat; Disease.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1048710
Registros recuperados: 18
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