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Watermelon transformation with Zucchini yellow mosaic virus coat protein gene and comparison with parental cultivar PAB
Çürük,Sebahattin; Meşe,Ebru.
The objective of this work was to transfer Zucchini yellow mosaic virus coat protein (ZYMV-CP) and neomycin phosphotransferase II (NPT II) genes to the watermelon 'Crimson Sweet'(CS) genome, and to compare the transgenic progenies T1 and T2 with the nontransformed parental cultivar for morphological, pomological, growth and yield characteristics. The ZYMV-CP gene was transferred by Agrobacterium tumefaciens. The presence of the gene in transgenic T0, T1 and T2 plants was determined by polymerase chain reaction, and the results were confirmed by Southern blot. Two experiments were performed, one in the winter-spring and the other in the summer-autumn. In both experiments, the hypocotyl length of transgenic seedlings was significantly higher than that of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrullus lanatus; Comparative safety analysis; Genetic modification; Neomycin phosphotrans-ferase II; Nontransgenic watermelon; Transgenic watermelon.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000100010
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