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Registros recuperados: 25
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A study of autophagy in hemocytes of the Pacific oyster, Crassostrea gigas ArchiMer
Picot, Sandy; Morga, Benjamin; Faury, Nicole; Chollet, Bruno; Dégremont, Lionel; Travers, Marie-agnes; Renault, Tristan; Arzul, Isabelle.
Macroautophagy is a mechanism that is involved in various cellular processes, including cellular homeostasis and innate immunity. This pathway has been described in organisms ranging in complexity from yeasts to mammals, and recent results indicate that it occurs in the mantle of the Pacific oyster, Crassostrea gigas. However, the autophagy pathway has never been explored in the hemocytes of C. gigas, which are the main effectors of its immune system and thus play a key role in the defence of the Pacific oyster against pathogens. To investigate autophagy in oyster hemocytes, tools currently used to monitor this mechanism in mammals, including flow cytometry, fluorescent microscopy and transmission electron microscopy, were adapted and applied to the...
Tipo: Text Palavras-chave: Autophagosome; Autophagy; Crassostrea gigas; Flow cytometry; Fluorescence microscopy; Hemocytes; Transmission electron microscopy.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00487/59905/63087.pdf
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Anatomical and biochemical changes associated with the development and germination of Araucaria angustifolia seeds Biological Sciences
Shibata, Marília; Coelho, Cileide Maria Medeiros; Schmidt, Eder Carlos; Bouzon, Zenilda Laurita; Campos, José Marcello Salabert de; Maraschin, Marcelo.
This study aimed at assessing the cell cycle, and anatomical and biochemical changes that the embryonic axis of Araucaria angustifolia undergoes during development, focusing on the maturation stage. During all development, cells exhibited intense metabolic activity with an abundance of mitochondria, lipid bodies, and vacuolated cells. The continued accumulation of starch and protein was observed by LM and TEM and indicated by spectra of FTIR. Cell differentiation of the procambium was observed with a thickening of the cell wall and the formation of resiniferous ducts. At Stage III and IV, cells exhibited structural changes such as altered or elongated mitochondria and presence of plastoglobules. These results suggest that there is a gradual transition from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0 Botânica; 2.03.03.00-9 Fisiologia Vegetal; 5.02.01.04-2 Sementes Florestais maturation; Recalcitrant seed; Light microscopy; Transmission electron microscopy; Flow cytometry Botânica.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43381
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Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia ArchiMer
Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle.
Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010...
Tipo: Text Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf
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Chloroplast ultrastructure and hormone endogenous levels are differently affected under light and dark conditions during in vitro culture of Guadua chacoensis (Rojas) Londoño & P. M. Peterson. Repositório Alice
POLESI, L. G.; FRAGA, H. P. F. de; VIEIRA, L. do N.; HERINGER, A. S.; ORNELLAS, T. S.; SANTOS, H. P. dos; GUERRA, M. P.; PESCADOR, R..
Abstract: Guadua chacoensis (Poaceae) is a woody bamboo native from the Atlantic forest biome. Morphogenetic and physiological studies are scarce in bamboos, and tissue culture-based biotechnologies tools can be used to investigate ultrastructure and physiological processes as well as to mass-propagate specific genotypes. This study evaluated the effect of light and dark conditions on chloroplast biogenesis as well as in the endogenous levels of zeatin (Z), abscisic acid (ABA), gibberellic acid (GA4), and jasmonic acid (JA) during in vitro culture of G. chacoensis. An increase was observed, followed by a decrease in starch content in response to light treatment, and in contrast, in darkness, an accumulation of starch which is associated to amyloplast...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Phytohormone; Guadua chacoensis (Poaceae); Woody bamboo native; Atlantic forest biome; Bamboos; Micropropagation; Transmission electron microscopy; Plant physiology.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103488
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Cryptosporidiosis and Isosporosis among AIDS Patients with Chronic Diarrhea in Thailand OAK
Wanachiwanawin, Darawan; Manatsathit, Sathaporn; Suwanagool, Parvinee; Tunsupasawasdikul, Somsith; Lertlaituan, Punpob; Thakerngpol, Kleophant.
Palavras-chave: Cryptosporidiosis; Isosporosis; AIDS patients; Chronic diarrhea; Transmission electron microscopy.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/274
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Cytopathology of callus cells infected with grapevine leafroll-associated virus 3 Trop. Plant Pathol.
SCAGLIUSI,SANDRA M. M.; VEGA,JORGE; KUNIYUKI,HUGO.
The cytopathology of grapevine (Vitis spp.) callus tissue infected with Grapevine leafroll-associated virus 3 (GLRaV-3), genus Vitivirus was studied in order to investigate the usefulness of callus cultures to study grapevine leafroll-associated viruses. Ultrathin sections were made from in vitro callus obtained from stems and shoots of GLRaV-3 infected grapevine plants. Callus was composed of two types of tissue. Translucent, soft callus was formed and composed of large loosely arranged cells, containing big vacuoles and a thin layer of cytoplasm. Other parts of the callus were brown-coloured and composed of small compactly arranged cells, which showed flexuous and rod-shaped closterovirus-like particles, with 10-12 nm in diameter, at higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Closterovirus; Vitis spp.; Transmission electron microscopy.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000400008
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Detection of a mollicute-like organism in kuruma shrimp, Penaeus japonicus ArchiMer
Choi, Dong-lim; Park, Myoung-ae; Sohn, Sang-gyu; Heo, Moon-soo; Renault, Tristan.
A filamentous form of mollicute-like bacterium was detected during a routine health survey of wild adult kuruma shrimp Penaeus japonicus. The kuruma shrimp were native to Japan and were imported to Korea. The histology showed no pathological changes. Transmission electron microscopy revealed extensive infections of hepatopancreatic epithelial cells by a pleomorphic, filamentous intracellular bacterium. The filamentous bacterium was of about 60 nm in diameter and 300 nm to more than 1µm in length. The morphology of bacteria were filamentous and branched with terminal blebs, or knobs, on the branches. They lacked the cell wall, and were bounded by the plasma membrane. They contained typical prokaryotic ribosomes and fibrillar DNA-like strands. No additional...
Tipo: Text Palavras-chave: Transmission electron microscopy; Hepatopancreas; Mollicute; Intracellular bacteria; Penaeus japonicus.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/1996/publication-2901.pdf
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Detection of Coronavirus in Capybaras (Hydrochoeris hydrochaeris) by Transmission Electron Microscopy in São Paulo, Brazil International Journal of Morphology
Catroxo,M. H. B; Miranda,L.B; Lavorenti,A; Petrella,S; Melo,N. A; Martins,A. M. C. P. R. F.
Coronaviruses are enveloped, positive-stranded RNA, belongs to the Nidovirales order, Coronaviridae family and are classified into three groups. Groups 3a and 3c comprising the avian coronaviruses. They are worldwide in distribution and can cause respiratory, enteric and in some cases hepatitis and neurological diseases in a wide variety of animals, resulting in economic losses to breeders and breeding. In this study, we describe an outbreak of diarrhea in a herd of capybaras found in the city of Piracicaba, São Paulo, Brazil. The animals had sudden symptoms of anorexia, profuse and watery diarrhea, yellowish or greenish, dehydration, weight loss, sharp and twisting of limbs leading to death in less than a week, mostly weaned and with an age of three...
Tipo: Journal article Palavras-chave: Coronavirus; Capybaras; Transmission electron microscopy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200035
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Ecophysiological, anatomical and ultrastructural characteristics of Vitex polygama Cham. (Verbenaceae) submitted to different concentrations of fluoride Biological Sciences
Rossi, Zélia Terezinha Teixeira; Castro, Evaristo Mauro de; Pereira, Giuslan Carvalho; Barbosa, João Paulo Rodrigues Alves Delfino; Carvalho, Graziele Wolff de Almeida; Rossi, Marco Aurélio de Lima Silva; Pereira, Fabrício José.
Fluorides released during the manufacture of aluminum, fertilizers, glass and ceramics are considered to be highly toxic to plants, causing damages in relatively small concentrations. This study aimed to evaluate fluoride (F) accumulation and its effects on Vitex polygama Cham. (Verbenaceae) exposed to simulated rain containing potassium fluoride in concentrations of 5, 10, 15 and 20 mg L-1. Vitex polygama presented no visual symptoms of foliar injury and accumulated a relatively small amount of F-, significant only for the treatments with higher concentrations of the pollutant.  F- promoted an increase in non-photochemical quenching (qN) and in the coefficient due to non-photochemical extinction (NPQ), which indicates higher dissipation of radiant energy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0; 2.03.02.03-7; 2.03.03.00-9 chlorophyll a fluorescence; Potassium fluoride; Scanning electron microscopy; Transmission electron microscopy; Biomonitoring studies Botânica; Anatomia Vegetal; Fisiologia Vegetal.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31400
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Herpesvirus infection in European flat oysters Ostrea edulis obtained from brood stocks of various geographic origins and grown in Galicia (NW Spain) ArchiMer
Da Silva, P; Renault, Tristan; Fuentes, J; Villalba, A.
We evaluated differences in productive traits and disease susceptibility among Ostrea edulis stocks. We produced 4 to 5 families from each of 4 oyster populations (Irish, Greek and 2 Galician) in a hatchery. Spat corresponding to 19 different families were transferred to a raft in the Ria de Arousa (Galicia, Spain) for grow-out. Samples of each family were histologically processed every month for 2 yr. One of the pathological conditions disclosed by histological examination was characterised by the occurrence of numerous abnormal cells throughout the connective tissue of various organs, showing hypertrophied nuclei with marginated chromatin and a characteristic large intranuclear acidophilic inclusion. Ultrastructural examination showed that the abnormal...
Tipo: Text Palavras-chave: In situ hybridisation; Transmission electron microscopy; Histology; Bonamiosis; Ostreid herspesvirus 1; Ostrea edulis.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3735.pdf
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Identification of Poxvirus Under Transmission Electron Microscopy During Outbreak Period in Wild Birds, in São Paulo, Brazil International Journal of Morphology
Catroxo,M. H. B; Pongiluppi,T; Melo,N. A; Milanelo,L; Petrella,S; Martins,A. M. C. P. F; Rebouças,M. M.
Avianpox is a highly contagious disease infecting both commercial and wild birds, causing great damages to breeders and breeding. Caused by DNA viruses of the family Poxviridae, genus Avipoxvirus, if manifest through 3 forms, cutaneous, diphtheric and septicemic. In June 2003, during illegal commercialization of Brazilian birds, 800 wild birds (Paroaria dominicana, Sporophila caerulescens and Sporophila albogularis) were apprehended and being forwarded to the CRAS (Wild Animals Recovery Center), Tietê Ecological Park. After one month, birds presented cutaneous lesions in the beak and feet and anorexia, emaciation, locomotion difficulties, diarrhea, dehydration and death. Among the 800 birds, 500 died and 40 these (15 Paroaria dominicana, 15 Sporophila...
Tipo: Journal article Palavras-chave: Poxvirus; Paroaria dominicana; Sporophila caerulescens; Sporophila albogularis; Transmission electron microscopy.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200043
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Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã - doi: 10.4025/actasciagron.v34i4.14501 Agronomy
Ribeiro, Luciene Oliveira; Universidade Federal de Lavras; Paiva, Luciano Vilela; Universidade Federal de Lavras; Pádua, Marlúcia Souza; Universidade Federal de Lavras; Santos, Breno Régis; Universidade Federal de Alfenas; Alves, Eduardo; Universidade Federal de Lavras; Stein, Vanessa Cristina; Universidade Federal de Goiás.
This work was carried out to characterise morphologically and ultrastructurally the banana callus, obtained from the scalp method. Genotypes of banana, cv. Prata anã, cultivated in vitro were used to induce meristems at the leaf base; subsequently, structures known as scalps were formed. For ultrastructural analysis, five samples of callus were collected, fixed in Karnovsky solution and analysed by scanning electron microscope (SEM) and transmission electron microscope (TEM). The formation of three types of callus was observed: Type 1 - transparent watery callus, Type 2 - yellow callus with small clusters, Type 3 - yellow callus with large clusters. SEM analysis showed that Type 1 callus cells were elongated and that Type 2 and Type 3 callus cells were...
Tipo: Laboratory analysis Palavras-chave: 5.01.00.00-9 Agronomia 5.01.00.00-9 scanning electron microscopy; Transmission electron microscopy; Musa sp.; Cytology; Embryogenesis Agronomia.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/14501
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Morphological study of partridge Eimeria procera development in the foreign host - Leghorn chicks (Gallus gallus) OAK
Revajová, Viera; Loószová, Adriana; Goldová, Maria; Zibrín, Martin; Herich, Robert; Levkut, Mikulas.
In order to morphological study of non-specific host immune response, cross-species experimental infection of Leghorn chicks with partridge specific coccidium, Eimeria procera (106 oocyst/per birds) was done. Schizonts of E. procera were histologically observed in all the parts of small intestine and caeca of chicks. Heterophilic and lymphocytic infiltration in lamina propria, and damage of villi intestine were observed. Schizonts were studied by electron microscope after 12, 30, and 60 hours post-infection (h pi). Results demonstrated immature and structurally damaged schizonts at 12 h pi, delayed maturation of first generation schizonts at 30 h pi, and phagocytosis of parasites at 60 h pi by intraepithelial lymphocytes.
Palavras-chave: Eimeria procera; Transmission electron microscopy; Specific and non-specific host; Intraepithelial lymphocytes.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/144
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Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer
Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis.
There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal...
Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf
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Recovery in culture of viable but nonculturable Vibrio parahaemolyticus: regrowth or resuscitation? ArchiMer
Coutard, Francois; Crassous, Philippe; Droguet, Michael; Gobin, Eric; Colwell, Rita; Pommepuy, Monique; Hervio Heath, Dominique.
The objective of this study was to explore the recovery of culturability of viable but nonculturable ( VBNC) Vibrio parahaemolyticus after temperature upshift and to determine whether regrowth or resuscitation occurred. A clinical strain of V. parahaemolyticus Vp5 was rendered VBNC by exposure to artificial seawater ( ASW) at 4 degrees C. Aliquots of the ASW suspension of cells ( 0.1, 1 and 10 ml) were subjected to increased temperatures of 20 degrees C and 37 degrees C. Culturability of the cells in the aliquots was monitored for colony formation on a rich medium and changes in morphology were measured by scanning ( SEM) and transmission ( TEM) electron microscopy. Samples of VBNC cells were fixed and examined by SEM, revealing a heterogeneous population...
Tipo: Text Palavras-chave: Transmission electron microscopy; Scanning electron microscopy; Resuscitation; Regrowth; Vibrio parahaemolyticus; VBNC state.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3117.pdf
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Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy International Journal of Morphology
Catroxo,M. H. B; Martins,A. M. C. R. P. F; Petrella,S; Curi,N. A; Melo,N.A.
The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae...
Tipo: Journal article Palavras-chave: Paramyxovirus; Adenovirus; Coronavirus; Pigeons; Transmission electron microscopy.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200055
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Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei Biocell
Zukas,Randi; Chang,Alex J.; Rice,Marian; Springer,Amy L..
Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the a heavy chain, and IC138, an intermediate chain, cause severe motility defects including immotility. To determine if motility defects result from structural...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flagellar motility; Transmission electron microscopy; Central pair.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300006
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The host-parasite relationship between the rainbow trout (Oncorhynchus mykiss) and the ectoparasite Argulus foliaceus (Crustacea: Branchiura): epithelial mucous cell response, cortisol and factors which may influence parasite establishment Naturalis
Nolan, D.T.; Salm, A.L. van der; Wendelaar Bonga, S.E..
The effects of short-term infection with the branchurian crustacean ectoparasite Argulus foliaceus, and the fish stress hormone cortisol (which is reported to stimulate mucus discharge), were studied on the mucous cell population of the head skin of rainbow trout (Oncorhynchus mykiss). Argulus infection did not raise plasma cortisol significantly and had no effect on the number of epidermal mucous cells in the head skin. Cortisol was administered twice to groups of trout via the food, significantly elevating circulating plasma cortisol at 24 h post feeding without affecting numbers of mucous cells, and increasing the numbers of vesicles in the upper cells of the epidermis. Subsequent infection with the parasite (6 Argulus f/fish) did not affect either...
Tipo: Article / Letter to the editor Palavras-chave: Ectoparasites; Host-parasite interaction; Skin epidermis; Cortisol; Mucous cells; Transmission electron microscopy.
Ano: 2000 URL: http://www.repository.naturalis.nl/record/534370
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Transmission Electron Microscopy Study of the Neonatal Rat Tongue Mucosa Treated with Special Attention to the Bacteriae on the Epithelial Cell Membrane International Journal of Morphology
Picoli,Lara Cristina; Lopes,Ruberval A; Semprini,Marisa; Sala,Miguel Angel; Ogawa,Koichi; Watanabe,Ii-sei.
The epithelial cell surfaces of neonatal Wistar rat tongue mucosa were examined by transmission electron microscopic method. The tissues were fixed by modified Karnovksy solution and embedded in Spurr resin. Thin sections of 90 nm thickness were cut and examined in the Jeol 1010 transmission electron microscopy. It was revealed that the queratinized squamous epithelial cells of the tongue mucosa exposed to cadmium presented groupings of numerous bacteriae which are attached to the flat cell membrane surfaces. The coccus and bacillus are attached to the cell membrane in organized rows or at random, as clearly seen in the TEM images. At high magnification, the transmission electron microscopic images showed that bacteriae are attached by a fibrillar...
Tipo: Journal article Palavras-chave: Epithelial cell; Tongue mucosa; Bacteria; Cadmium; Transmission electron microscopy.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300006
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Ultrastructural Analysis of Masseter and Left Medial Pterigoid Muscles, After Unilateral Mandibular Molar Extraction: Experimental Study International Journal of Morphology
Benigno,Maria Ivone Mendes; Liberti,Edson Aparecido; Conde Jr,Airton Mendes; de Sousa Leal,Noélia Maria; Lopes Rodrigues,Karla Emanuelle; Mota,Vanessa Benigno; Amstalden,Eliane Maria Ingrid.
Twelve adult male Wistar rats (220 g average weight) were divided in 3 experimental groups: GI -15. GII ­ 30 and GIII ­ 60 days, after mandibular molar extraction, with three experimental animals and one control per group. Qualitatively, ultrastructural changes of protein filaments from myofibrils of these muscles and ipsilateral to the extractions were observed. Ultrastructure asymmetry and disorganization of Z line and I band, in the experimental group GII, of Medial Pterigoid muscle (MPT) were observed. The temporomandibular dysfunction, stimulated by the unilateral extractions of mandibular molars in rats may lead to modifications in the Z line and I band, which showed to be sensitive to this dysfunction. Changes in the MPT muscle, probably related to...
Tipo: Journal article Palavras-chave: Skeletal muscular fibre; Masticator muscles; Occlusal alterations; Temporomandibular joint; Transmission electron microscopy.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200007
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