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Action of plant proteinase inhibitors on enzymes of physiopathological importance Anais da ABC (AABC)
Oliva,Maria Luiza V.; Sampaio,Misako U..
Obtained from leguminous seeds, various plant proteins inhibit animal proteinases, including human, and can be considered for the development of compounds with biological activity. Inhibitors from the Bowman-Birk and plant Kunitz-type family have been characterized by proteinase specificity, primary structure and reactive site. Our group mostly studies the genus Bauhinia, mainly the species bauhinioides, rufa, ungulata and variegata. In some species, more than one inhibitor was characterized, exhibiting different properties. Although proteins from this group share high structural similarity, they present differences in proteinase inhibition, explored in studies using diverse biological models.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bowman-Birk; Chymotrypsin; Kunitz inhibitors; Plasma kallikrein; Primary structure; Trypsin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300023
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Activities of selected digestive enzymes during larval development of large yellow croaker (Pseudosciaena crocea) ArchiMer
Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen.
Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,...
Tipo: Text Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf
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Adaptation of digestive enzymes to dietary protein, carbohydrate and fibre levels and influence of protein and carbohydrate quality in Penaeus vannamei larvae (Crustacea, Decapoda) ArchiMer
Le Moullac, Gilles; Van Wormhoudt, A; Aquacop, Aquacop.
Activity of digestive enzymes (trypsin, chymotrypsin and amylase) was studied in relation with food in Penaeus vannamei larvae first stages. Microparticles containing casein involved the decrease of soluble protein content and enzyme activities. Specific trypsin activity but not chymotrypsin was correlated (p<1%) to casein level between 10 and 60% in microparticles. Cellulose fibres in food seemed to have little effect on digestive enzymes. Starch, between 1 and 20% in microparticles, had no influence on specific amylase activity. The effect of different feeding conditions were also tested on growth. No clear relationship between growth and enzyme is established since only trypsin is concerned in the case of a casein dose effect in the food. The protein...
Tipo: Text Palavras-chave: Amylase; Chymotrypsin; Trypsin; Digestive enzymes; Food; Larvae; Penaeus vannamei; Amylase; Chymotrypsine; Trypsine; Enzymes digestives; Alimentation; Larve; Penaeus vannamei.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-4431.pdf
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Aqueous extract of Psidium guajava leaves: phenolic compounds and inhibitory potential on digestive enzymes Anais da ABC (AABC)
SIMÃO,ANDERSON A.; MARQUES,TAMARA R.; MARCUSSI,SILVANA; CORRÊA,ANGELITA D..
ABSTRACT Leaves of Psidium guajava L. (guava) have been widely used in the popular way for prevention and treatment of various diseases. Thus, the objective of this study was to evaluate the inhibitory potential of leaves aqueous extract from three cultivars of P. guajava (Pedro Sato, Paluma and Século XXI) on α-amylase, α-glycosidase, lipase, and trypsin enzymes, in the presence or not of simulated gastric fluid and to determine the content of phenolic compounds by high performance liquid chromatography. All cultivars presented the same composition in phenolic compounds, but in different proportions. The compounds identified are gallic acid, epigallocatechin gallate, syringic acid, o-coumaric acid, resveratrol, quercetin, and catechin (which was the major...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Obesity; Α-amylase; Α-glycosidase; Lipase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502155
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Chymotrypsin- and trypsin-like activities secreted by the multidrug-resistant yeasts forming the Candida haemulonii complex Anais da ABC (AABC)
SOUTO,XÊNIA M.; BRANQUINHA,MARTA H.; SANTOS,ANDRÉ L.S..
Abstract: Candida haemulonii complex (C. haemulonii, C. haemulonii var. vulnera and C. duobushaemulonii) consists of emergent multidrug-resistant pathogens that cause bloodstream and deep-seated infections. However, little is known about their virulence factors. Herein, we evaluated the presence of extracellular serine peptidases in this fungal complex. Serine peptidase activity was measured by spectrophotometry using chromogenic peptide substrates to the S1 family. Chymotrypsin-, trypsin- and elastase-like activities were detected in all fungal isolates. Since higher chymotrypsin- and trypsin-like activities were observed from the cleavage of N-succinyl-Ala-Ala-Pro-Phe-pNa and N-benzoyl-Phe-Val-Arg-pNa, respectively, these substrates were selected for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brazilian clinical isolates; Candida haemulonii complex; Chymotrypsin; Serine peptidases; Trypsin; Virulence factors.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500803
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Composition of gastrointestinal content, protease and lipase activities in summer and winter of four freshwater siluriforms (Teleostei: Actinopterygii) with two different feeding habits Rev. Bras. Zool.
Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322
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Detection of trypsin inhibitor in seeds of Eucalyptus urophylla and its influence on the in vitro growth of the fungi Pisolithus tinctorius and Rhizoctonia solani BJM
Tremacoldi,Célia Regina; Pascholati,Sérgio Florentino.
Inhibitors of plant proteases can regulate the hydrolysis of proteins inside the cells and also participate in the mechanisms of plant defense against herbivore insects and pathogens. Here, we demonstrated that seeds of Eucalyptus urophylla exhibit activities of trypsin and papain inhibitors, two proteases commonly found in living cells. Low amounts of proteins of the crude protein extract of seeds and fractions partially purified by gel filtration, with inhibitory activity against trypsin, inhibited in vitro the mycelial growth of a compatible isolate of the ectomycorrhizal fungus Pisolithus tinctorius and allowed an unsatisfactory growth of another isolate from Pinus taeda, considered incompatible for this eucalyptus species. The same amounts of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Inhibitor; Trypsin; Pisolithus tinctorius; Rhizoctonia solani.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400001
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Determining how the pelagic ecosystem over the continental shelf of the Bay of Biscay (NE Atlantic) functions: An approach using mesozooplankton enzyme activities as descriptors ArchiMer
Bergeron, Jean-pierre; Delmas, Daniel; Koueta, Noussithe.
A fisheries research cruise conducted in 2000 offered a first opportunity to take simultaneous measurements of the activities of three enzymes in mesozooplankton samples collected at a regional scale over the continental shelf of the Bay of Biscay in the NE Atlantic, with the aim of characterizing main aspects of the functioning of the biotic environment of small pelagic fish populations. The activity of the digestive endopeptidase trypsin was selected to characterize the assimilation rate of proteins, whereas pyruvate kinase (PK) was chosen as an indicator of carbohydrate assimilation and aspartate transcarbamylase (ATC) provided an overall assessment of mesozooplankton productivity. The Bay of Biscay region is subject to various strong physical driving...
Tipo: Text Palavras-chave: Trypsin; Pyruvate kinase; Metabolic descriptors; Mesozooplankton; Bay of Biscay; Aspartate transcarbamylase.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6359.pdf
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Different feeding habits influence the activity of digestive enzymes in freshwater fish Ciência Rural
Gioda,Carolina Rosa; Pretto,Alexandra; Freitas,Carine de Souza; Leitemperger,Jossiele; Loro,Vania Lucia; Lazzari,Rafael; Lissner,Leandro Ademar; Baldisserotto,Bernardo; Salbego,Joseânia.
ABSTRACT: The aim of this study was to verify the activity of some digestive enzymes in four fish species with different feeding habits. Knowledge of these enzymatic activities can help us to understand the species' digestive processes. The species chosen for this study were Ctenopharyngodon idella (herbivore), Rhamdia quelen (omnivore), Leporinus obtusidens (omnivore) and Hoplias malabaricus (carnivore). The digestive tract of these species was divided into four portions to estimate enzymatic activity: stomach, anterior, mid and posterior intestine. Ctenopharyngodon idella had the highest amylase and maltase activities in all portions of the gut, followed by L. obtusidens . The highest trypsin activity was observed in all gut portions of H. malabaricus,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Freshwater fishes; Feeding habits; Enzymatic activity; Amylase; Alkaline protease; Maltase; Trypsin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300406
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Eficiencia da farinha desengordurada de gergelim como complemento proteico da farinha extrudada de caupi. Repositório Alice
MAIA, G.A.; CALVETE, Y.M. de A.; TELLES, F.J.S.; MONTEIRO, J.C.S.; SALES, M.G..
O objetivo deste trabalho foi promover uma avaliacao nutricional da mistura proteica desengordurada, obtida do gergelim (Sesamum indicum L.), com a farinha extrudada do caupi (Vigna unguiculata L. Walp), utilizando-se metodos fisicos, bioquimicos, nutricionais e sensoriais. Usando-se semente integral do gergelim, com 54,08% de oleo e 21,83% de proteina, obteve-se a semente descascada com baixa concentracao de oxalatos, que foi usada para a producao de farinha desengordurada de gergelim (FDG) contendo 2,81% de oleos e 59,16% de proteina. A principal caracteristica desta farinha foi sua alta concentracao de aminoacidos sulfurados (30,88 mg/g proteina). Essa FDG foi usada como complemento da proteina da farinha do caupi obtida pelo processo de cozimento por...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sesamum indicum; Vigna unguiculata; Oxalatos; Aminoacidos sulfurados; Tripsina; Oxalates; Sulphur amino acids; Trypsin.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/89615
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Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. Repositório Alice
PRADO, J. S. M.; LOURENÇÃO, A. L.; GUEDES, R. N. C.; PALLINI, A.; OLIVEIRA, J. A.; OLIVEIRA, M. G. A..
Ingestion of proteinase inhibitors leads to hyperproduction of digestive proteinases, limiting the bioavailability of essential amino acids for protein synthesis, which affects insect growth and development. However, the effects of proteinase inhibitors on digestive enzymes can lead to an adaptive response by the insect. In here, we assessed the biochemical response of midgut proteinases from the eucalypt defoliator Thyrinteina arnobia (Stoll) to different concentrations of berenil, a bisbenzamidine proteinase inhibitor, on eucalyptus. Eucalyptus leaves were immersed in berenil solutions at different concentrations and fed to larvae of T. arnobia. Mortality was assessed daily. The proteolytic activity in the midgut of T. arnobia was assessed after feeding...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Synthetic inhibitor; Trypsin; Pest control.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/953013
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Glyphosate on digestive enzymes activity in piava (Leporinus obtusidens) Ciência Rural
Salbego,Joseânia; Pretto,Alexandra; Silva,Vera Maria Machado da; Loro,Vania Lucia; Lazzari,Rafael; Gioda,Carolina Rosa; Baldisserotto,Bernardo.
The effects of glyphosate, a nonselective herbicide (1.0 or 5.0mg L-1) on digestive enzymes activity (stomach and intestine) were evaluated in juveniles of piava (Leporinus obtusidens) after 90 days of exposure. The activity of acid protease, trypsin, chymotrypsin and amylase increased with the increase of glyphosate concentration. These results indicate that glyphosate affects digestive enzyme activities in this species, and may be an indicator of poor nutrient availability when fish survive in herbicide-contaminated water.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Herbicide; Acid protease; Amylase; Trypsin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000901603
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Influence of dietary live yeast on European sea bass (Dicentrarchus labrax) larval development ArchiMer
Tovar Ramirez, Dariel; Zambonino-infante, Jose-luis; Cahu, Chantal; Gatesoupe, Joel; Vazquez Juarez, R.
Recent results have demonstrated that live yeast in diets improved gut maturation in sea bass larvae. In this study, we tested the absence and two levels of live yeast in sea bass larvae diets. Specimens were fed from time of mouth opening to 37 days after hatching a diet of 0%, 1.1%, or 5.7% wet weight of live yeast (Debaryomyces hansenii CBS 8339). Yeast incorporation improved survival 10%, and reduced malformed larvae. In groups fed 1.1% yeast, only 1% of larvae were malformed, compared to 14% in the control group. Final mean weight in groups fed 1.1% yeast was twice that of other groups. Activities and concentrations of mRNA trypsin and lipase were higher in the two groups fed yeast than in the control group, whereas activity and concentration of mRNA...
Tipo: Text Palavras-chave: Polyamines; Lipase; Trypsin; Amylase; Enzymes; Gut maturation; Fish larvae.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2004/publication-831.pdf
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Nutritional condition of anchovy Engraulis encrasicolus larvae in connection with mesozooplankton feeding catabolism in the southern Bay of Biscay, NE Atlantic ArchiMer
Bergeron, Jean-pierre.
The study examines the spatial variations over the French continental shelf of the southern Bay of Biscay (North-East Atlantic) in the nutritional condition of anchovy (Engraulis encrasicolus L.) larvae which was estimated through their DNA/C index. The same cruise yielded quantitative estimates of two metabolic descriptors of the functioning of mesozooplanktonic communities, the enzymes pyruvate kinase (PK) and trypsin, for the first time evaluated simultaneously. The aim was to appraise quality and quantity of matter assimilated by the mesozooplankton and to specify whether the main components of prey ingested by copepods are principally carbohydrates, through PK activity, or protein, under the action of the digestive endopeptidase trypsin. The larval...
Tipo: Text Palavras-chave: Anchovy larvae; Bay of Biscay; DNA/C index; Mesozooplankton; Pyruvate kinase; Trypsin.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6473.pdf
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Purification and characterisation of trypsin-like enzyme from the pyloric caeca of cod (Gadus morhua) II BABT
Beirão,Luiz Henrique; Mackie,Ian Mckintoch; Teixeira,Evanilda; Damian,César.
A trypsin -like enzyme from the pyloric caeca of cod (Gadus morhua) was purified by affinity chromatography on CHOM Sepharose 4B. Some characteristics were established by its catalytic activity on T.A.M.E., typical enzyme substrate, and serine protease inhibitors. The enzyme had an isoelectric point of 5.30 and 5.89 and was very similar in amino acid composition to bovine trypsin, but differed in having a higher relative amount of acidic amino acids and a lower amount of basic amino acids. The enzyme also hydrolysed fish protein substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cod; Enzyme; Trypsin.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000100005
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Reactivity of anti-thyroid antibodies to thyroglobulin tryptic fragments: comparison of autoimmune and non-autoimmune thyroid diseases BJMBR
Boechat,L.H.B.; Zollner,R.L..
Studies concerning the antigenicity of thyroglobulin fragments allow the characterization of the epitopes but do not consider the role of heavier antigenic fragments that could result in vivo from the action of endoproteases. Here we assess the relative importance of the fragments obtained from thyroglobulin by limited proteolysis with trypsin and compare by immunoblotting their reactivity to serum from patients with autoimmune (Graves' disease and Hashimoto's thyroiditis) and non-autoimmune (subacute thyroiditis) disease. The results showed no difference in frequency of recognition of any peptide by sera from patients with autoimmune thyroiditis. In contrast, sera from patients with subacute thyroiditis reacted more frequently with a peptide of 80 kDa....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autoantibodies; Autoimmunity; Thyroglobulin; Trypsin; Thyroiditis; Proteases.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400012
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Role of Chicken Pancreatic Trypsin, Chymotrypsin and Elastase in the Excystation Process of Eimeria tenella Oocysts and Sporocysts OAK
Guyonnet, Vincent; Johnson, Joyce K.; Long, Peter L..
The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS) added with 0.5% chicken bile extract in a 5% C02 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella (Expt.1). No excystation was observed under these conditions. Sporocysts were also incubated under the same conditions without pretreatment in C02. Excystation was observed for sporocysts incubated with either trypsin, chymotrypsin or pancreatic elastase, the best percentage of excystation being...
Palavras-chave: Chymotrypsin; Eimeria tenella; Elastase; Excystation; Trypsin.
Ano: 1991 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/155
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Thermodynamic evaluation and modeling of proton and water exchange associated with benzamidine and berenil binding to ß-trypsin BJMBR
Pereira,M.T.; Silva-Alves,J.M.; Martins-José,A.; Lopes,J.C.D.; Santoro,M.M..
Serine-proteases are involved in vital processes in virtually all species. They are important targets for researchers studying the relationships between protein structure and activity, for the rational design of new pharmaceuticals. Trypsin was used as a model to assess a possible differential contribution of hydration water to the binding of two synthetic inhibitors. Thermodynamic parameters for the association of bovine ß-trypsin (homogeneous material, observed 23,294.4 ± 0.2 Da, theoretical 23,292.5 Da) with the inhibitors benzamidine and berenil at pH 8.0, 25ºC and with 25 mM CaCl2, were determined using isothermal titration calorimetry and the osmotic stress method. The association constant for berenil was about 12 times higher compared to the one for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Benzamidine; Berenil; Calorimetry; Protein modeling; Trypsin; Osmotic stress.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100005
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