Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 6
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Method for obtaining high-resolution proteomic analysis from pericarps of guarana. Repositório Alice
SOUZA, A. L.; ANGELO, P. C. da S.; NOGUEIRA, P. P. O.; GONÇALVES, J. F. C.; FRANCO, A. M.; ASTOLFI-FILHO, S.; LÓPEZ-LOZANO, J. L.; ANDRADE, E. V..
Guarana has great agricultural potential and is largely used therapeutically and in the production of non-alcoholic energy drinks. Genomic and proteomic studies are crucial to identify proteins that play central roles in the maintenance and viability of fruits, as well as to identify proteins related to the main metabolic pathways. However, the success of any protein analysis starts with the protein extract preparation, which needs to offer an extract that is free of contaminants. This study aimed to evaluate different extraction methods to obtain high-quantity and high-quality extracts that are compatible with analysis by 2-dimensional electrophoresis and tandem mass spectrometry protein identification. Three different methods were tested: trichloroacetic...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Guaraná; Paullinia cupana; Plant proteomics; Two-dimensional electrophoresis; Polyphenols.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/996859
Imagem não selecionada

Imprime registro no formato completo
Protein profile of rice (Oryza sativa) seeds Genet. Mol. Biol.
Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping.
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012
Imagem não selecionada

Imprime registro no formato completo
Proteomic analysis of Rhizobium freirei PRF 81T reveals the key role of central metabolic pathways in acid tolerance. Repositório Alice
TULLIO, L. D.; GOMES, D. F.; SILVA, L. P. da; HUNGRIA, M.; BATISTA, J. S. da S..
bitstream/item/194799/1/1-s2.0-S0929139318305936-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Abiotic stresses; Biological nitrogen fixation; PH homeostasis; Acid consuming; Two-dimensional electrophoresis; Oxidative stress.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1107373
Imagem não selecionada

Imprime registro no formato completo
Proteomic analysis of whey from bovine colostrum and mature milk BABT
Golinelli,Luciana Pacheco; Conte-Junior,Carlos Adam; Paschoalin,Vânia Margaret Flosi; Silva,Joab Trajano.
The aim of this study was to standardize a methodology to obtain two-dimensional (2D) maps of whey proteins from bovine colostrum and mature milk, using two-dimensional electrophoresis, in order to identify the minor proteins by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS). A total of 38 proteins were identified, 20 spots in the colostrum whey and 18 in the mature milk whey; 5 of them were identified for the first time.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine whey proteins; Colostrum proteins; Low-abundance proteins; Two-dimensional electrophoresis; Mass spectrometry; Matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI TOF/TOF MS).
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400016
Imagem não selecionada

Imprime registro no formato completo
Proteomic inventory of myocardial proteins from patients with chronic Chagas' cardiomyopathy BJMBR
Teixeira,P.C.; Iwai,L.K.; Kuramoto,A.C.K.; Honorato,R.; Fiorelli,A.; Stolf,N.; Kalil,J.; Cunha-Neto,E..
Chronic Chagas' disease cardiomyopathy (CCC) is an often fatal outcome of Trypanosoma cruzi infection, with a poorer prognosis than other cardiomyopathies. CCC is refractory to heart failure treatments, and is the major indication of heart transplantation in Latin America. A diffuse myocarditis, plus intense myocardial hypertrophy, damage and fibrosis, in the presence of very few T. cruzi forms, are the histopathological hallmarks of CCC. To gain a better understanding of the pathophysiology of CCC, we analyzed the protein profile in the affected CCC myocardium. Homogenates from left ventricular myocardial samples of end-stage CCC hearts explanted during heart transplantation were subjected to two-dimensional electrophoresis with Coomassie blue staining;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagas' disease; Cardiomyopathy; Proteomic analysis; Two-dimensional electrophoresis; MALDI-ToF.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200005
Imagem não selecionada

Imprime registro no formato completo
STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis Genet. Mol. Biol.
Qiu,Qing-chao; Hu,Bo; He,Xiu-pei; Luo,Qiao; Tang,Guo-hua; Long,Zhi-feng; Chen,Zhu-chu; He,Xiu-sheng.
STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CNE2 cell line; Nasopharyngeal carcinoma; Nude mouse; STGC3; Tet-on; Two-dimensional electrophoresis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100002
Registros recuperados: 6
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional