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Registros recuperados: 27
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Adaptation de la méthode peptide-spot à la recherche de sites, de la protéine RNA2 du nodavirus, reconnus par différents sérums de loup (Dicentrarchus labrax). ArchiMer
Coeurdacier, Jean-luc.
Spot-Synthesis technique was applied to study protein frame or to develop antibodies with specific characteristics for use in diagnostic or research on human or mammals. In this work this technique was used to find sites on RNA2 protein of Nodavirus which are immunogenic for sea bass (Dicentrarchus labrax). It aimed to achieve synthetic peptide vaccine production against this virus. One hundred and nine overlapping peptides, of 15 amino acids frameshifted by three residues, representing the complete RNA2 protein sequence of Sb1 and Sb2 nodavirus were synthesized on a cellulose membrane by the Spot-Technique. The set of membrane bound peptides was probed by incubation with sea-bass or rabbit serum. Anti sea bass or anti rabbit alkaline phosphatase...
Tipo: Text Palavras-chave: Peptides synthétiques; Vaccin; Peptid-spot; Séquence immunogène; Dicentrarchus labrax; Synthetic peptides; Vaccine; Immunogenicity; Dicentrarchus labrax...
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00157/26810/24919.pdf
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Avaliação genética das vacinas contra a brucelose bovina comercializadas no Brasil. Repositório Alice
BASTOS, R.; SOARES, C. O.; ELISEI, C.; MUNHOZ, A. L. R.; BEZERRA, N. L.; CAITANO, M. A. B.; ROSINHA, G. M. S..
Objetivou-se com este estudo caracterizar genotipicamente as amostras vacinais B19 e RB51 comercializadas no Brasil.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Brucella abortus; Brucellosis; Vaccine; B19; RB51; Genotyping; Genetic quality.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937237
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Avaliação hematológica de ovinos imunizados com glicoproteina instestinal de Haemonchus contortus. Repositório Alice
MATOS, A. F. M.; SANTOS, T. L. B. A.; FONTENELE, J. A.; SIEBRA, P. S. C.; ALBUQUERQUE, F. H. M. A. R. de.; TEIXEIRA, M..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Raça Santa Inês; Nematóide; Helminto gastrintestinal; Hematologia; Vacina; Parasitismo; Haemonchus contortus; Ovino; Imunização; Vaccine; Sheep; Parasitism; Nematoda; Hematology; Immunization.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1009946
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Candidacy of liver stage antigen-1 for Plasmodium falciparum vaccine development OAK
Taylor-Robinson, Andrew W.; Heal, Karen G..
Malaria is the world's most important parasitic disease, imposing a massive health burden on people living in the tropics, often in the poorest countries. The vast majority of deaths in humans from malaria are caused by one species of the hemoprotozoan, Plasmodium falciparum., against which effective control measures are urgently needed. The global situation has deteriorated in recent times due to increased resistance of the anopheline mosquitoes that transmit P. falciparum to insecticides, and of the parasites themselves to drug therapy. An efficacious and cost-effective vaccine against this parasite is considered a public health priority. A vaccine that targets pre-erythrocytic parasites in the liver could potentially prevent clinical disease by blocking...
Palavras-chave: Malaria; Plasmodium falciparum; Liver stage; Vaccine; Cellular immunity.
Ano: 2001 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/133
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Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1 OAK
Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343
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Control of Newcastle Disease in Village Chickens AgEcon
Centre for International Economics.
Between 1983 and 1992, the Australian Centre for International Agricultural Research (ACIAR) invested A$3 million in research to find a vaccine that could provide protection from Newcastle disease in chickens and be applied in village environments in developing countries. A further $160 000 was invested in follow up projects which ended in 1996. Village chickens often provide the only source of protein to poor villagers living in remote areas and Newcastle disease frequently devastates unvaccinated village flocks. The ACIAR-sponsored research was highly successful in developing a heat resistant vaccine (HRV4) which could be readily used in the field by coating it onto chicken feed. The vaccine was commercialised by an Australian company which subsequently...
Tipo: Book Palavras-chave: Newcastle disease; Chickens; Poultry; Vaccine; Malaysia; Vietnam; ACIAR; Australia; Developing country; Village; Africa; Farm Management; Food Consumption/Nutrition/Food Safety; Food Security and Poverty; International Development; Livestock Production/Industries; Research and Development/Tech Change/Emerging Technologies; Research Methods/ Statistical Methods.
Ano: 1998 URL: http://purl.umn.edu/47190
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Effect of Oligomannose-Coated Liposome-Based Vaccine on Rodent Babesiosis OAK
Ishigame, T; Takabatake, N; Iseki, H; Ota, N; Koyama, A; Igarashi, I; Nishikawa, Y; Ikehara, Y; Kojima, N; Yokoyama, N; 五十嵐, 郁男; 西川, 義文; 横山, 直明.
Oligomannose-coated liposome (OML)-based vaccines have been reported to induce Th1-based immunity against entrapped antigens in immunized animals and to show protective effects against several protozoal diseases. In the present study, we produced a recombinant Babesia rodhaini ribosomal phosphoprotein P0 (rBrP0) that showed immunological cross reactivity with another rodent Babesia parasite, B. microti. We evaluated the efficacy of vaccination with OML-entrapped rBrP0 on B. rodhaini and B. microti infections in mice. Prior immunization with the OML-based rBrP0 vaccine, or with a Freund’s adjuvant-based rBrP0 vaccine, failed to demonstrate any protective effect against lethal infection with B. rodhaini, but the OML-based vaccine did induce protective...
Palavras-chave: Oligomannose-coated liposomes (OML); Vaccine; Babesiosis; Babesia rodhaini; Babesia microti; P0.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2383
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Etiology of childhood community acquired pneumonia and its implications for vaccination BJID
Nascimento-Carvalho,Cristiana M.C..
Pneumonia is an important cause of morbidity and mortality among children throughout the world. Vaccines are available for some organisms, but they are underutilized and/or still in development. To evaluate the potential impact of vaccines, we review studies in which the etiology of childhood community-acquired pneumonia was recorded. In North America and Europe (9 studies), the etiology of pneumonia was established in 62% of studied children (range 43%-88%) by use of noninvasive specific methods for microbiologic diagnosis. The most often identified agents were S. pneumoniae (22%), respiratory syncytial virus (RSV) (20%), Haemophilus influenzae (7%), and Mycoplasma pneumoniae (15%). In Africa and South America (8 studies), bacteria were recovered from 56%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Community-acquired pneumonia; Children; Etiology; Vaccine; S. pneumoniae; Respiratory syncytial virus.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000200007
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Expression of Babesia gibsoni thrombospondin-related adhesive protein in Toxoplasma gondii and evaluation of its antigenicity and immunogenicity OAK
Kumagai, A.; Zhang, G.; Jia, H.; Nakamura, C.; Zhang, H.; Goo, Y-K.; Aboge, G. A.; Terkawi, M. A.; Zhou, J.; Nishikawa, Y.; Xuan, X..
A gene encoding Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP), known as a vaccine candidate, was stably expressed in Toxoplasma gondii (Tg/BgTRAP). The molecular weight and the antigenic reaction of recombinant BgTRAP expressed by the Tg/BgTRAP were similar to the original ones expressed by B. gibsoni. To evaluate the antigenicity of the recombinant BgTRAP expressed by T. gondii, the lysates of the recombinant parasite tachyzoites were intraperitoneally injected into mice. The serum collected from Tg/BgTRAP-immunized mouse could react to B. gibsoni parasites, while the serum collected from wild-type T. gondii tachyzoites (Tg/wt)-immunized mice did not. These results indicate that T. gondii could provide a new tool to produce foreign...
Palavras-chave: Toxoplasma gondii; Babesia gibsoni; BgTRAP; Expression; Vaccine.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4496
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Formula to evaluate efficacy of vaccines and systemic substances against three-host ticks. Repositório Alice
AGUIRRE, A. de A. R.; BARROS, J. C.; ANDREOTTI, R..
2015
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Vaccine; Tick; Three-host; Efficacy.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1022409
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Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis. Repositório Alice
RAMOS, C. A. N.; ARAUJO, F. R.; ALVES, L. C.; SOUZA, I. I. F. de; GUEDES JUNIOR, D. S.; SOARES, C. O..
Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genetic conservation; Babesia bovis; Vaccine; Brazil.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937283
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Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus OAK
AIHARA, Naoyuki; HORIUCHI, Noriyuki; HIKICHI, Nanase; OCHIAI, Mariko; HOSODA, Yuko; ISHIKAWA, Yoko; SHIMAZAKI, Yoko; OISHI, Koji; 堀内, 雅之.
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine...
Palavras-chave: Bursal follicle; Infectious bursal disease; Vaccine.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4148
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Interleukin-12 and protective immunity to schistosomes BJMBR
Mountford,A.P.; Shires,V.L.; Anderson,S..
The attenuated vaccine against Schistosoma mansoni induces Th1-mediated protective immunity and we have sought to identify a role for IL-12 in this model. Elevated levels of IL-12 (p40 mRNA) were detected in the lymph nodes (LN) and the lungs of vaccinated mice, whilst treatment of vaccinated mice with anti-IL-12 antibodies decreased the ratio of IFN<FONT FACE="Symbol">g</font>:IL-4 secreted by in vitro-cultured LN cells. However, there was only marginal abrogation of the level of resistance in these mice. Soluble antigens from the lung-stage of the parasite (SLAP) appeared to be efficient stimulators of IFN<FONT FACE="Symbol">g</font> and IL-12 secretion. These antigens when used to immunise mice in conjunction with IL-12 as an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Schistosoma; Interleukin-12; Adjuvant; Vaccine; Th1.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000100023
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Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14 Electron. J. Biotechnol.
Moura Leal,Maria de Lourdes; da Silva Gomes Pereira,Denise; Jessouroun,Ellen; Peixoto Gimenes Couto,Maria Antonieta; Pereira Jr,Nei.
Streptococcus pneumoniae (pneumococcus) is among the most significant causes of bacterial disease in humans. Capsular polysaccharide (CPS) production is essential for pneumococcal virulence. Pneumococcal CPS has been widely used as vaccine antigen. This study is focused on the influence of culture conditions of Streptococcus pneumoniae serotype 14 as for developing an industrial method for polysaccharide production. The pH proved to be a highly important variable in batchwise culture. Using the pH control all glucose added was consumed resulting in a four-fold increase in polysaccharide productivity relative to cultivation without pH control. S. pneumoniae is a lactic acid bacterium, so named for its primary metabolic byproduct (lactate), which has an...
Tipo: Journal article Palavras-chave: Polysaccharide capsular; Vaccine; Streptococcus pneumoniae.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000500006
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Malaria vaccine: roadblocks and possible solutions BJMBR
Soares,I.S.; Rodrigues,M.M..
Malaria remains the most prevalent and devastating parasitic disease worldwide. Vaccination is considered to be an approach that will complement other strategies for prevention and control of the disease in the future. In the last 10 years, intense studies aimed at the development of a malaria vaccine have provided important knowledge of the nature of the host immunological mechanisms of protection and their respective target antigens. It became well established that protective immune responses can be generated against the distinct stages of Plasmodium. However, in general, protective immune responses are directed at stage-specific antigens. The elucidation of the primary structure of these antigens made possible the generation of synthetic and recombinant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malaria; Plasmodium; Vaccine; Immunity; Infectious disease.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300002
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Nanoparticles and microparticles of polymers and polysaccharides to administer fish vaccines Biol. Res.
Rivas-Aravena,Andrea; Sandino,Ana María; Spencer,Eugenio.
Aquaculture has become an important economic sector worldwide, but is faced with an ongoing threat from infectious diseases. Vaccination plays a critical role in protecting commercially raised fish from bacterial, viral and parasitic diseases. However, the production of effective vaccines is limited by the scarcity of knowledge about the immune system of fish. Improving vaccines implies using antigens, adjuvants and employing methods of administration that are more effective and less harmful to the fish. In this context, in recent year there have studies of methods of encapsulating antigens in matrices of different types to apply in fish vaccines. This work reviews the new methods to improve fish vaccines by encapsulating them in polymers and...
Tipo: Journal article Palavras-chave: Nanoparticles; Vaccine; Aquaculture.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000400012
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Partial budget analysis of prepartum antimicrobial therapy and Escherichia coli J5 vaccination of dairy heifers and their effect on milk production and milk quality parameters. Repositório Alice
VARGAS, R. T.; SOUZA, F. N.; BRITO, M. A. V. P. e; BRITO, J. R. F.; LEITE, M. O.; FONSECA, L. M.; SAMPAIO, I. B. M.; CERQUEIRA, M. M. O. P..
Abstract: This study aimed to determine whether prepartum antimicrobial and/or Escherichia coli J5 vaccination in dairy heifers influence the milk production, milk quality, and estimate their economic benefit. Thus, 33 dairy heifers were enrolled in four groups using a split-splot design. Groups were: (G1) prepartum antimicrobial infusion and vaccination with an E. coli J5 bacterin, (G2) prepartum antimicrobial infusion, (G3) vaccination with an E. coli J5 bacterin, and (G4) control heifers. Composite milk samples for somatic cell count, total bacteria count and milk composition were collected 15 days after calving and every 15 days until the end of the experiment. Bacteriological analysis was carried out at the end of study. The milk production and the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Mastitis; Milk; Antimicrobial treatment; Vaccine; Dairy cows.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1061512
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Peroxiredoxin 3 promotes IL-12 production from macrophages and partially protects mice against infection with Toxoplasma gondii OAK
Fereig, Ragab M.; Nishikawa, Yoshifumi.
Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the...
Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569
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POLYMERASE CHAIN REACTION DETECTION OF AVIAN LEUKOSIS VIRUS DNA IN VACCINES USED IN POULTRY Rev Salud Anim.
Acevedo,Ana María; Rodríguez,Edisleidy; Uffo,Odalys; Relova,Damarys; Noda,Julia; Díaz de Arce,Heidy.
Avian leukosis viruses (ALV) provoke a variety of trasmissible bening and malign tumoral diseases affecting birds. Chickens are affected by six subgroups of ALV designs A, B, C, D, E and J of more recent world dissemination. These viruses are potential contaminants of live vaccines used in poultry. In order to research the presence of DNA from ALVs as contaminants of viral commercial vaccines to be used in poultry, different Marek´s disease vaccines were screened by a reported polymerase chain reaction (PCR) assay designed to detect all subgroups of ALVs. DNA samples extracted from seven vaccines were submitted to PCR using primers for a conserved region of env gene of HPRS-103. ALV sequences were detected in seven samples (100%). The methodology employed...
Tipo: Journal article Palavras-chave: Avian leukosis virus (ALV); Polymerase chain reaction (PCR); Vaccine; Contamination; Poultry.
Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000100011
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Quando e como vacinar contra a tristeza parasitaria. Repositório Alice
KESSLER, R. H.; SCHENK. M. A. M..
Epidemiologia. Como prevenir a tristeza? Quando vacinar?
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Sanidade animal; Tristeza parasitária; Babesiose; Anaplasmose; Vacina; Brasil; Animal health; Tickborne diseases; Babesiosis; Anaplasmosis; Vaccine; Brazil.
Ano: 2000 URL: http://www.alice.cnptia.embrapa.br/handle/doc/325244
Registros recuperados: 27
Primeira ... 12 ... Última
 

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