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Registros recuperados: 8
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Campagne d’évaluation du stock de palourdes du bassin d’Arcachon. Année 2018 ArchiMer
Sanchez, Florence; Caill-milly, Nathalie; Lissardy, Muriel.
This report details the results of clam stock assessment survey, which took place between 14 May and 17 June 2018 in Arcachon Bay. Co-organized with CDPMEM33, with financial support from DLAL FEAMP Arcachon Basin Val de l'Eyre, Nouvelle-Aquitaine region, the CDPMEM33 and Ifremer, the clam stock assessment and its characteristics were provided. The samples were collected from 533 sampling stations distributed over an area of 52 km², with an Hamon grab, using a generalized random tessellation stratified spatially balanced design (GRTS). Venerupis philippinarum remains the dominant species with 99 % of the population. The size distribution of Manila clam is in the range 5 – 41 mm with a main mode around 24-28 mm. In 2018, the mean densities are 39 individuals...
Tipo: Text Palavras-chave: Évaluation de stock; Palourde japonaise; Venerupis philippinarum; Bassin d’Arcachon Key words: stock assessment; Manila clam; Venerupis philippinarum; Arcachon Bay.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00477/58897/61456.pdf
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Development of a Taqman real-time PCR assay for rapid detection and quantification of Vibrio tapetis in extrapallial fluids of clams ArchiMer
Bidault, Adeline; Richard, Gaelle G.; Le Bris, Cedric; Paillard, Christine.
The Gram-negative bacterium Vibrio tapetis is known as the causative agent of Brown Ring Disease (BRD) in the Manila clam Venerupis (=Ruditapes) philippinarum. This bivalve is the second most important species produced in aquaculture and has a high commercial value. In spite of the development of several molecular methods, no survey has been yet achieved to rapidly quantify the bacterium in the clam. In this study, we developed a Taqman real-time PCR assay targeting virB4 gene for accurate and quantitative identification of V. tapetis strains pathogenic to clams. Sensitivity and reproducibility of the method were assessed using either filtered sea water or extrapallial fluids of clam injected with the CECT4600(T) V. tapetis strain. Quantification curves of...
Tipo: Text Palavras-chave: Vibrio tapetis; VirB4 gene; Taqman real-time PCR; Molecular diagnostic; Venerupis philippinarum; Marine pathogen; Brown ring disease.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00308/41909/73194.pdf
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Immune responses of phenoloxidase and superoxide dismutase in the manila clam Venerupis philippinarum challenged with Vibrio tapetis – Part I: Spatio-temporal evolution of enzymes’ activities post-infection ArchiMer
Le Bris, Cedric; Richard, Gaelle; Paillard, Christine; Lambert, Christophe; Seguineau, Catherine; Gauthier, Olivier; Pernet, Fabrice; Guerard, Fabienne.
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains: CECT4600T, the causative agent of Brown Ring Disease (BRD); and LP2 supposedly non-pathogenic in V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major enzymes involved in immunity, were studied in two tissues, the mantle and hemolymph for 30 days after infection in the extrapallial cavity. Bacterial infection in V. philippinarum resulted in modulation of PO and SOD activities that was both tissue- and time-dependent. A response at early times was detected in the mantle and was associated with significant increases in PO and SOD activities in LP2- and CECT4600T-challenged clams 36h post injection....
Tipo: Text Palavras-chave: Venerupis philippinarum; Vibrio tapetis; Immunity; Phenoloxidase; Superoxide dismutase.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00221/33257/31733.pdf
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Morphological Trends of Four Manila Clam Populations (Venerupis philippinarum) on the French Atlantic Coast: Identified Spatial Patterns and Their Relationship to Environmental Variability ArchiMer
Caill-milly, Nathalie; Bru, Noelle; Barranger, Melanie; Gallon, Laurent; D'Amico, Franck.
For species' dynamics highly influenced by environmental conditions, sustainable management for conservation and/or exploitation purposes requires the ability to segregate natural processes from anthropogenic impacts. In this context, identification of phenotypic variations in morphological patterns, and clarification of the mechanisms involved, constitute useful tools to identify adequate management measures from Europe-wide to local conditions. Although such approaches are already used effectively in the management of fish stocks, they are more recent for bivalve stocks, which are also good candidates for such management tools. The current study examines morphological patterns in the Manila clam (Venerupis philippinarum) through morphometric studies of 4...
Tipo: Text Palavras-chave: Shell shape; Linear morphometric methods; Contour detection; Environmental effect; Linear multilevel model; Bivalve; Atlantic Ocean; Venerupis philippinarum.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00225/33605/32004.pdf
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Optimization of a survey using spatially balanced sampling: a single-year application of clam monitoring in the Arcachon Bay (SW France) ArchiMer
Kermorvant, Claire; Caill-milly, Nathalie; D'Amico, Frank; Bru, Noelle; Sanchez, Florence; Lissardy, Muriel; Brown, Jennifer.
Bivalves are important components of benthic marine and freshwater ecosystems throughout the world. One of the most exploited bivalves used for human consumption is manila clam (Venerupis philippinarum). In Arcachon Bay (SW France), commercial fishers and scientists have developed a monitoring survey to estimate clam stocks to assist in implementing a sustainable management strategy. The survey design that is currently used is based on standard stratified random sampling (StRS). The survey has been undertaken every 2 years since 2006. Each survey costs approximately (sic) 50 000, with funding provided by similar to 20% of the commercial fishers. The survey is quite expensive, given that this resource is managed mostly at a regional level. In 2016 for...
Tipo: Text Palavras-chave: Venerupis philippinarum; Bivalve; Simulation; Survey; Arcachon Bay; GRTS.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00410/52102/52808.pdf
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Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene.
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the...
Tipo: Text Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni..
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf
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Relations entre l’état d’une ressource et son exploitation via la compréhension et la formalisation des interactions de socio-écosystèmes. Application à la palourde japonaise (Venerupis philippinarum) du bassin d’Arcachon. ArchiMer
Caill-milly, Nathalie.
Significant component of the marine benthic ecosystem, exploited bivalves are mainly located inside the coastal area, which temporal and spatial features generate natural population fluctuations and contribute to considerable biomass and demographic structure changes added to anthropogenic activities’ effects (fishing, pollution,…). Understanding of the relationships between the species, its environment and its exploitation is crucial and their formalization, including for management purposes, requires development of mathematical models that aim at describing the functioning of the system, to explain the observed phenomenon in terms of cause and effect or predict effects of new causes. Originating from venerid culture trials carried out in France in the...
Tipo: Text Palavras-chave: Bivalve; Morphométrie; Allométrie; Dynamique des populations; Système dynamique; Gestion; Palourde japonaise; Venerupis philippinarum; Bivalve; Morphometry; Allometry; Population dynamics; Dynamic system; Management; Manila clam; Venerupis philippinarum.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00139/25034/23143.pdf
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Vibrio tapetis Displays an Original Type IV Secretion System in Strains Pathogenic for Bivalve Molluscs ArchiMer
Dias, Graciela M.; Bidault, Adeline; Le Chevalier, Patrick; Choquet, Gwenaelle; Sarkissian, Clio Der; Orlando, Ludovic; Medigue, Claudine; Barbe, Valerie; Mangenot, Sophie; Thompson, Cristiane C.; Thompson, Fabiano L.; Jacq, Annick; Pichereau, Vianney; Paillard, Christine.
The Brown Ring Disease (BRD) caused high mortality rates since 1986 in the Manila clam Venerupis philippinarum introduced and cultured in Western Europe from the 1970s. The causative agent of BRD is a Gram-Negative bacterium, Vibrio tapetis, which is also pathogenic to fish. Here we report the first assembly of the complete genome of V. tapetis CECT4600T, together with the genome sequences of 16 additional strains isolated across a broad host and geographic range. Our extensive genome dataset allowed us to describe the pathogen pan-and core genomes and to identify putative virulence factors. The V. tapetis core genome consists of 3,352 genes, including multiple potential virulence factors represented by haemolysins, transcriptional regulators, Type I...
Tipo: Text Palavras-chave: Vibrio tapetis; Venerupis philippinarum; Comparative genomics; Pathogenicity; T4SS; Pangenome; Core genome.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54077/72091.pdf
Registros recuperados: 8
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