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Registros recuperados: 6
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Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus BJM
Abdallah,Fethi Ben; Chaieb,Kamel; Zmantar,Tarek; Kallel,Hela; Bakhrouf,Amina.
In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio; Slime production; Adherence; Abiotic surface; Vero cells.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200033
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Evaluation of cytotoxicity and wound healing activity of Avicennia schaueriana in cream Anais da ABC (AABC)
LOPES,CAROLINE M.I.; BARATELLA-EVÊNCIO,LIRIANE; SOUZA,IVONE A. DE; OLIVEIRA,ERWELLY B. DE; SÁ,JÉSSICA G.A.; SANTANA,MARLLON A.N.; MARCELINO NETO,PEDRO P.; SANTANA,EDUARDA S. DE; SILVA,LUZIA A. DA; VIEIRA,JEYMESSON R.C..
Abstract:Avicennia schaueriana is found in Brazilian mangrove coast. The cytotoxicity in vitro of the Aqueous Extract of Leaves of Avicennia schaueriana (AELAs) and the healing activity of the plant in cream on mice skin wounds were evaluated in this study. The cytotoxic evaluation was performed on Vero cells. The healing activity was evaluated on mice treated during 5, 10 and 15 days with cream at 5%, solution of sodium chloride at 0.9% and dexpanthenol in cream at 5%. The extract did not show cytotoxicity, but showed mitogenic activity (100μg/ml). In morphometric analysis, the percentage of wound contraction after 10 days was higher in dexpanthenol group (93.41%). In 15 days, the lowest percentage of contraction was observed in the dexpanthenol group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avicennia schaueriana; In vitro techniques; Re-epithelialization; Vero cells; Wound healing.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100805
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Influence of culture conditions on Vero cell propagation on non-porous microcarriers BABT
Souza,Marta Cristina de Oliveira; Freire,Marcos da Silva; Castilho,Leda dos Reis.
Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vero cells; Microcarriers; Cytodex 1; Cytodex 3; High-cell-density cultivation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400009
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Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells BJMBR
Ishimaru,D.; Marcicano,F.G.P.; Rebello,M.A..
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Prostaglandins; Mayaro virus; Vero cells; Protein synthesis.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900003
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Use of blends of bioabsorbable poly(L-lactic acid)/poly(hydroxybutyrate- co-hydroxyvalerate) as surfaces for Vero cell culture BJMBR
Santos Jr.,A.R.; Ferreira,B.M.P.; Duek,E.A.R.; Dolder,H.; Wada,M.L.F..
Vero cells, a cell line established from the kidney of the African green monkey (Cercopithecus aethiops), were cultured in F-10 Ham medium supplemented with 10% fetal calf serum at 37°C on membranes of poly(L-lactic acid) (PLLA), poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and their blends in different proportions (100/0, 60/40, 50/50, 40/60, and 0/100). The present study evaluated morphology of cells grown on different polymeric substrates after 24 h of culture by scanning electron microscopy. Cell adhesion was also analyzed after 2 h of inoculation. For cell growth evaluation, the cells were maintained in culture for 48, 120, 240, and 360 h. For cytochemical study, the cells were cultured for 120 or 240 h, fixed, processed for histological analysis,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly(L-lactic acid); Poly(hydroxybutyrate- co-hydroxyvalerate); Blends; Biomaterials; Cell culture; Vero cells.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100009
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Virulence and cytotoxicity of seafood borne Aeromonas hydrophila BJM
Illanchezian,Seethalakshmi; Jayaraman,SathishKumar; Manoharan,Muthu Saravanan; Valsalam,Saritha.
The present study was conducted to determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, India. Among 73 A. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on Vero cells. Cell shrinkage, detachment and rounding of Vero cells were recorded as cytotoxic changes. Only one strain did not show haemolysis, slime production, proteolytic activity and cytotoxicity on treatment with Vero cells. Positive correlation was observed between proteolytic activity and cytotoxicity irrespective of haemolytic activity of the strains. These results...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aeromonas hydrophila; Seafood; Virulence; Cytotoxicity; Vero cells.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400016
Registros recuperados: 6
Primeira ... 1 ... Última
 

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