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ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS Rev. Microbiol.
Silva,Simone Helena da; Vieira,Enio Cardillo; Nicoli,Jacques Robert.
In an ex vivo agar plate assay, we monitored the appearance of an inhibitory halo against Vibrio cholerae from the feces of Wistar and Fischer rats aged 10 to 42 days. The frequency of Wistar rats showing halo increased from 0% (10 days) to a maximum of 80.0% (29 days) and then decreased to 53.3% (42 days). A similar pattern was obtained with Fischer rats but with a lower intensity (maximum frequency of 50.0% by day 36). In a separate experiment, when Wistar rats were fed a low-protein diet for 7 days, the inhibitory halo decreased drastically. Three apparently different colony morphologies were isolated from the dominant fecal microbiota: a facultative anaerobe (FAN) and two strict anaerobes (SAN). The ex vivo inhibitory test showed a halo around the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio cholerae; Escherichia coli; Streptococcus intermedius; Antagonism; Rodents.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300016
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Bactéries pathogènes indigènes des eaux estuariennes: cas des vibrios. Revue bibliographique ArchiMer
Fouche, Dominique.
De nombreuses bactéries, peuplant habituellement les zones estuariennes, se révèlent être de plus en plus fréquemment des agents de maladies dont les vecteurs principaux sont les coquillages et leur consommation par l'homme. Le présent travail synthétise l'état des connaissances sur les vibrios, en particulier Vibrio cholerae, Vibrio parahaemolyticus et Vibrio vulnificus, bactéries pour lesquelles il existe une  abondante littérature. L'écologie, le pouvoir pathogène et les milieux de culture les plus couramment utilisés en vue de leur détection sont présentés. Enfin, leur interaction avec les mollusques bivalves, leur survie dans ceux-ci et le problème de l'épuration conséquente de ces derniers sont abordés.
Tipo: Text Palavras-chave: Vibrio cholerae; Vibrio parahaemolyticus; Vibrio vulnificus; Bactéries indigènes; Estuaires; Bivalves; Purification.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00408/51929/52554.pdf
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Ecología de Vibrio cholerae en relación al Fitoplancton y variables fisicoquímicas en ríos de Tucumán (Argentina) Boletín de la Sociedad
Mirande,V.; Tracanna,B. C.; Seeligmann,C . T.; Cangemi,R.; Aulet,O.; Cecilia,M.; Silva,C.; Binsztein,N..
Vibrio cholerae muestra gran diversidad serológica en base a su antígeno somático O, conociéndose al menos 200 serogrupos. De éstos, solamente O1 y O139 son causantes de epidemias o pandemias. En Latinoamérica el serogrupo O1 reapareció en 1991, tras cien años de no presentar brotes en el continente. Esta bacteria sobrevive y se multiplica asociada al plancton, independientemente de la aparición de infecciones humanas. Desde la década del noventa, en Tucumán, se detectaron casos esporádicos de diarrea por Vibrio cholerae no-O1. El objetivo del presente trabajo fue estudiar la posible relación entre la presencia de especímenes de fitoplancton, variables fisicoquímicas y aislamientos de Vibrio cholerae en ríos de Tucumán. Se realizaron 18 campañas en los...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fitoplancton; Reservorio; Vibrio cholerae; Fisicoquímica; Ríos; Tucumán.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000200007
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Effect of ionizing radiation onVibrio bacteria in Crassostrea virginica (American oyster) ArchiMer
Eberiel, D; Dooley, K; Langton, L; Malleth, J.
The presence of Vibrio bacteria in raw shellfish poses a significant health hazard to the consuming public. These human pathogens are natural, seasonal components of the microbial flora of shellfish which can result in primary septicemic or diarrheal fatal infections. Existing shellfish depuration neither targets nor eliminates the Vibrio bacteria. For this reason, additional purification processes are necessary. In this study, the effect of low dose gamma radiation on the inactivation ofVibrio cholerae (01 and non 01) and vulnificus is investigated. Log decrement (D10) values ranging only to a high of 0.09 KGy in the Vibrio strains support the hypothesis that food irradiation technology can provide an additional effective and efficient shellfish...
Tipo: Text Palavras-chave: USA Coasts; USA; Vibrio vulnificus; Vibrio cholerae; Crassostrea virginica; Bivalvia; Vibrio; Bacteria; Self purification; Marine molluscs; Irradiation; Gamma radiation; Ionizing radiation; Oyster culture.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1619.pdf
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Effects of temperature abuse on survival ofVibrio vulnificus in oysters ArchiMer
Murphy, S; Oliver, J.
Of the several bacterial diseases which may result from consumption of shellfish, those caused by marine bacteria of the genus Vibrio are the most abundant. In the United States, up to 10,000 non-fatal cases per year are estimated to be caused by the various members of this genus. More than 95% of all deaths in the United States which result from seafood consumption are caused by a single bacterium, Vibrio vulnificus. The bacterium is a normal inhabitant of estuarine waters, and occurs naturally in especially high numbers in molluscan shellfish. Infections following consuption of raw or undercooked shellfish, especially oysters, result in fatality rates of over 60%. Because most oysters in the United States are transported large distances before marketing,...
Tipo: Text Palavras-chave: USA Coasts; Vibrio cholerae; Vibrio vulnificus; Crassostrea virginica; Bivalvia; Bacteria; Temperature effects; Food technology; Fish poisoning; Oyster culture.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-1622.pdf
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Estudo da sensibilidade in vitro de diferentes cepas de Vibrio cholerae 01 à radiação gama de 60Co Braz. J. Vet. Res. Anim. Sci.
MORAES,Ivany Rodrigues de; GELLI,Dilma Scala; JAKABI,Miyoko; MASTRO,Nélida Lúcia Del.
A presença de certos microrganismos nos alimentos ou de metabolitos originados durante sua multiplicação pode dar lugar a várias enfermidades no homem: as intoxicações e as infecções alimentares. Dentre os agentes causadores das Enfermidades Transmitidas por Alimentos encontra-se o Vibrio cholerae 01. Estudou-se no presente trabalho a radiossensibilidade in vitro de quatro cepas de V. cholerae 01, submetidas ao tratamento com diferentes doses de radiação ionizante de 60Co, e compararam-se os resultados obtidos com dados da literatura relacionados a problemas de veiculação alimentar de bactérias patogênicas, incluindo a água.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio cholerae; Doenças por alimentos; Radiação ionizante.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600001
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Etude ecologique des vibrions du bassin d'Arcachon ArchiMer
Marchand, Michel.
Water and sediment samples monthly collected from Arcachon Bay (Gironde, France) were examined during three years for the presence of marine vibrios. V. cholerae serotype 01 was never detected. On the other hand, the Eyre River and mouth contain non-agglutinating V. cholerae (127 strains) principally in water. The low temperatures slow their development down without stopping it completely. V. parahaemolyticus (257 strains) present in the same areas is particularly isolated from water when the aquatic medium temperature rises to 15 degree C. V. alginolyticus (638 strains) settles the whole Bay, except the river, in any season. The low temperatures promote growth of V. metschnikovii (203 strains) very tolerant towards salinity. V. anguillarum (46 strains)...
Tipo: Text Palavras-chave: Vibrio metschnikovii; Vibrio anguillarum; Vibrio alginolyticus; Vibrio cholerae; Vibrio; Marine environment; Microorganisms; Sediments; Salinity effects; Temperature effects; Ecology; Water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-998.pdf
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Fluorescent antibody enumeration of Vibrio cholerae in the marine environment. ArchiMer
Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D.
From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for...
Tipo: Text Palavras-chave: Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1000.pdf
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Real-Time PCR optimization to identify environmental Vibrio spp. strains ArchiMer
Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique.
Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V....
Tipo: Text Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf
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