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Gene silencing: concepts, applications, and perspectives in woody plants Scientia Agricola
Souza,Amancio José de; Mendes,Beatriz Madalena Januzzi; Mourão Filho,Francisco de Assis Alves.
RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanisms reveal similar biochemical pathways and appear to be related in several levels. The ability to manipulate gene silencing has produced transgenic plants able to switch off endogenous genes and invading nucleic acids. This powerful biotechnological tool has provided plant breeders and researchers with great opportunity to accelerate breeding programs and developmental studies in woody plants. This research work reports on gene silencing in woody plants, and discuss applications and future perspectives.
Tipo: Info:eu-repo/semantics/article Palavras-chave: RNAi; MiRNA; SiRNA; Genetic transformation; Virus resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000600014
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Morphological attributes and production components of potato cv. Baronesa and its transformed genotype - doi: 10.4025/actasciagron.v33i4.10918 Agronomy
Pohl, Simone; Universidade Federal de Pelotas; Lopes, Nei Fernandes; Universidade Federal de Pelotas; Braga, Eugenia Jacira; Universidade Federal de Pelotas; Danielowski, Rodrigo; Universidade Federal de Pelotas; Silva, Ilda Mariclei de Castro da; Universidade Federal de Pelotas; Peters, Jose Antonio; Universidade Federal de Pelotas.
The aim of this paper was to compare the morphological attributes and production components of the wild-type Baronesa cultivar potato with those of Baronesa potato plants that have been genetically modified using the potato virus Y coat protein (CP) gene. The experiment was conducted under greenhouse conditions (Biosecurity Quality Certificate no. 081/98 issued by the National Biosecurity Technical Committee) at the Federal University of Pelotas (UFPel), Pelotas-RS. The shoot and root lengths and the mean leaf number were determined six times at 14-day intervals after sowing; the length, width and fresh weight of the tubers were measured during the last harvest, which occurred 84 days after sowing. There were no statistical differences between the two...
Tipo: Pesquisa em casa de vegetação Palavras-chave: 5.01.03.00-8 Solanum tuberosum; Genetic modification; Virus resistance Fitotecnia Solanum tuberosum; Genetic modification; Virus resistance.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10918
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Overview of potyvirus resistance in watermelon National Institute of Agronomic Research
Guner, N.; Wehner, T.C..
Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is a major cucurbit crop that accounted for 7.5 % of the world area devoted to vegetable production in 2003. Potyviruses are a major limiting factor in commercial watermelon production worldwide. The major viruses affecting watermelon are Papaya ringspot viruswatermelon strain (PRSV-W), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). Sources of resistance to PRSV-W have been reported. These were PI (plant introduction) accessions from South Africa (PI 244017, PI 244018, PI 244019), from Zimbabwe (PI 482342, PI 482318, PI 482379), from Botswana (PI 485583), and from Nigeria (PI 595203). A single recessive gene controls resistance to PRSV-W, with the gene symbol prv....
Tipo: Poster Palavras-chave: ZYMV (Zucchini yellow mosaic virus); WMV (Watermelon mosaic virus); PRSV-W (Papaya ringspot virus watermelon strain); Citrullus lanatus; Potyvirus; Cucurbit; Virus resistance.
Ano: 2008 URL: http://hdl.handle.net/2174/245
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Variabilité génétique et évaluation génomique de la résistance à la nécrose nerveuse virale de populations sauvages de bar/loup (Dicentrarchus labrax) ArchiMer
Doan, Quoc Khanh.
European seabass is one of the most economic species in aquaculture in Mediterranean areas. Viral nervous necrosis (VNN), a disease affecting at least 70 aquatic species, has become the most serious threat to seabass cultured industry. While numerous studies have been performed in order to control this disease, no simple and effective procedures are available. In this thesis, we question genetic variability and the potential of selective breeding as an opportunity to address thwart this threat.After a general introduction (first chapter) and a deep literature review of nodavirus in aquaculture (second chapter), we explore in the third chapter the genetic variability of resistance of different wild populations of European seabass, namely Northern Atlantic...
Tipo: Text Palavras-chave: Génétique quantitative; Analyses génomiques; Pathologie; Résistance virale; Aquaculture; Quantitative genetics; Genomics; Pathology; Virus resistance; Aquaculture.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00498/60971/64379.pdf
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การปรับปรุงพันธุ์ต้านทานโรคไวรัสใบหงิกเหลืองของมะเขือเทศ Thai Agricultural
Kanlayanee Chaichana; Prasartporn Smitamana; Maneechat Nikronpun.
Improvement of tomato for resistance to tomato yellow leaf curl virus (TYLCV) were carried out byusing a pedigree and backcrossing methods and crossing between resistant parent H-24 with susceptible parents CT-1 and CT-2. The virus was transmitted by viruliferous whiteflies and virus infection was detected by sandwich ELISA. F2 generation showed moderate resistance with scores ranging from 1.58-3.21. Segregation of disease resistance was observed among the population. Virus detection of the F2 populations (scoring 1 or 2) showed virus titer which were not statistically different from H24 but were significantly different from CT-1, CT-2 and susceptible control. The disease severity and virus titer in infected plants of F2 populations showed positive...
Tipo: PhysicalObject Palavras-chave: Tomatoes; Tomato yellow leaf curl; Viroses; Plant breeding; Hybrid; Fertilization; Virus resistance; มะเขือเทศ; โรคใบหงิกเหลือง; โรคที่เกิดจากเชื้อไวรัส; การปรับปรุงพันธุ์พืช; ความต้านทานโรค; พันธุ์ลูกผสม; การคัดเลือกพันธุ์; วิธีการผสมพันธุ์.
Ano: 2009 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5720
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ความหลากหลายของยีนต้านทานไวรัส (Mx1) ในสุกร Thai Agricultural
Santi Pinthukas; Christoph Knorr; Ratchaneewan Kumphakam; Bertram Brenig; Kesinee Gatphayak.
Disease is a major problem in the swine production. The study of genes associated with disease resistance characteristics is used to select for innate immunity in swine. The Mx1 gene was found to have potent antiviral affect against some virus and can be hereditary. The study of Mx1 polymorphisms in among 3 pig breeds (Thai native pig breeds, Chinese pig breeds and European pig breeds) were found SNPs in exon 8 (c. 1022C>G; 1078A>C) that has allele frequencies of statistical significance between the breeds. (P<0.05) The two variant in exon 15 found silent point mutation (c. 2120G>T) และ 11-bp indels (c.2117_2128 indels CGGCGCCGGCT) that has allele frequencies of statistical significance in some breed (P<0.05)especially in German Landrace and...
Tipo: Collection Palavras-chave: Porcine; Gene; Mx1; Virus resistance; Porcine myxovirus resistance gene; Genetic marker; สุกร; ยีน; การต้านทานไวรัส; อัลลีล; ความหลากหลายทางพันธุกรรม; ตัวบ่งชี้ทางพันธุกรรม.
Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5189
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