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Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps BABT
Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes.
Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008
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Cell wall hydrolases in the seeds of Euphorbia heterophylla L. during germination and early seedling development Braz. J. Plant Physiol.
Suda,Cecilia N. K.; Buckeridge,Marcos S.; Giorgini,Jarbas F..
Activities of cell wall hydrolases of Euphorbia heterophylla L. (wild poinsettia) endosperm were investigated during pre- and post-emergence periods, defined as the time interval before and after 2.2 days from the start of imbibition, respectively. The activities of endo-beta-mannanase and beta-mannosidase are higher over the pre-emergence when compared to the post-emergence period and they may be involved in the process of germination in E. heterophylla. On the other hand, the activities of beta-galactosidase, beta-glucosidase, alpha-xylosidase, beta-xylosidase and glucanases, which hydrolyse CMC, xyloglucans from Hymenaea courbaril or Copaifera langsdorffii, xylan, Avicel and lichenan, are higher over the post-emergence period. Activity on laminarin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cotyledon expansion; Endosperm; Endoglucanase; Endomannanase; Euphorbiaceae; Xylanase.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000300002
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Cellulase and xylanase productions by isolated Amazon Bacillus strains using soybean industrial residue based solid-state cultivation BJM
Heck,Júlio X.; Hertz,Plinho F.; Ayub,Marco A.Z..
In Brazil, a large amount of a fibrous residue is generated as result of soybean (Glycine max) protein production. This material, which is rich in hemicellulose and cellulose, can be used in solid state cultivations for the production of valuable metabolites and enzymes. In this work, we studied the bioconversion of this residue by bacteria strains isolated from water and soil collected in the Amazon region. Five strains among 87 isolated bacteria selected for their ability to produce either celullases or xylanases were cultivated on the aforementioned residue. From strain BL62, identified as Bacillus subtilis, it was obtained a preparation showing the highest specific cellulase activity, 1.08 UI/mg protein within 24 hours of growth. Concerning xylanase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-State cultivation; Cellulase; Xylanase; Soybean residue; Amazon biodiversity.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300005
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Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli BJM
Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying.
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced. The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11 of glycosyl hydrolases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Gene cloning; Fusariumsp.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049
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Coexpression of rumen fungal xylanase and bifunctional cellulase genes in Escherichia coli BABT
Comlekcioglu,Ugur; Gunes,Merva; Altun,Hanifi; Ekiz,Dilek Ozgun; Aygan,Ashabil.
ABSTRACT Rumen fungi inhabit the gastro-intestinal tract of ruminants and the most non-ruminant herbivores. Rumen fungi produce highly active plant cell wall degrading enzymes, therefore they have gained scientific interest. In this study, genes encoding xylanase (xynA-7) and cellulase (celA-5) were amplified from Neocallimastix sp. GMLF7 and Orpinomyces sp. GMLF5, respectively, and expressed in Escherichia coli. XynA-7 was found to be active only on xylan, however CelA-5 had activity both on carboxymethyl cellulose and lichenan. Lichenase activity of CelA-5 was found to be higher than carboxymethyl cellulase activity. The optimal conditions were at pH 6.0 and 40 °C for CelA-5 and at pH 6.5 and 50 °C for XynA-7. A coexpression vector was constructed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rumen fungi; Xylanase; Cellulase; Coexpression; E. coli.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100407
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Comparative study for the kinetics of extracellular xylanases from Trichoderma harzianum and Chaetomium thermophilum Electron. J. Biotechnol.
Ahmed,Sibtain; Imdad,Syeda Sana; Jamil,Amer.
Xylanases assume special importance in the paper and pulp industry as they replace toxic chemicals such as chlorine and chlorine dioxide for developing eco-friendly processes. This study evaluated xylanases produced by two fungi, the mesophilic fungus Trichoderma harzianum and a thermophilic fungus Chaetomium thermophilum. Among the polymeric substrates studied for xylanase production by both the fungi, birch wood xylan was found to be the best inducer of xylanases. Xylanases induction was subject to glucose repression. Partially purified xylanases preparation from T. harzianum and C. thermophilum exhibited optimal activities at pH 5 and 6 and at 60ºC and 70ºC, respectively. The apparent Km and Vmax values for the partially purified xylanase from T....
Tipo: Journal article Palavras-chave: C. thermophilum; Kinetics; T. harzianum; Xylanase.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300003
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Corn and soybean meal metabolizable energy with the addition of exogenous enzymes for poultry Rev. Bras. Ciênc. Avic.
Dourado,LRB; Sakomura,NK; Barbosa,NAA; Bonato,MA; Kawuauchi,IM; Fernandes,JBK; Costa,FGP.
Two metabolism assays were carried out to determine corn and soybean meal metabolizable energy when enzymes were added. In the first trial, 35 cockerels per studied feedstuff (corn and soybean meal) were distributed in a completely randomized experimental design with four treatments of seven replicates of one bird each. The evaluated treatments were: ingredient (corn and soybean meal) with no enzyme addition, with the addition of an enzyme complex (xylanase, amylase, protease - XAP), xylanase, or phytase. Precise feeding method was used to determine true metabolizable energy corrected for nitrogen balance (TMEn). The use of enzymes did not result in any differences (p>0.05) in soybean meal TMEn, but phytase improved corn TMEn in 2.3% (p=0.004). In the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Forced feeding; Phytase; Protease; Xylanase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100008
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Effect of an Enzymatic Blend and Yeast on the Performance, Carcass Yield and Histomorphometry of the Small Intestine in Broilers from 21 to 42 Days of Age Rev. Bras. Ciênc. Avic.
Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC.
ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p<0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300
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Endoglucanase production with the newly isolated Myceliophtora sp. i-1d3b in a packed bed solid state fermentor BJM
Zanelato,A. I.; Shiota,V. M.; Gomes,E.; Silva,R. da; Thoméo,J. C..
This work is aimed to produce endoglucanase through solid state fermentation in a packed bed bioreactor with the use of the fungus Myceliophtora sp. I-1D3busing a mixture of wheat bran (WB) and sugar cane bagasse (SCB) as culture medium. Preliminary tests were performed in polypropylene plastic bags, controlling the variables temperature (40, 45, and 50ºC), initial moisture content (75, 80, and 85%, w.b.), and weight proportion SCB/WB (1:1, 7:3, and 9:1). The highest enzyme activities in plastic bags were obtained using the substrate proportion of 7:3, 50ºC temperature, and 80% initial moisture content (878 U/grams of dry solid). High activities of filter-paper cellulase and xylanase were also obtained in plastic bags and some results are reported. For the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Xylanase; Myceliophthora sp.; Packed bed; Solid state-fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400038
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Enzymatic programs for broilers BABT
Sorbara,José Otávio Berti; Murakami,Alice Eiko; Nakage,Eliane Saiuri; Piracés,Francisco; Potença,Alexandra; Guerra,Rafael Lachinski Holanda.
Two trials were conducted on floor pens to evaluate the performance of broilers fed with diets contained different carbohydrases enzyme programs at different ages. Trial 1- The body weight gain (BWG) was affected by the Negative Control (NC) treatment (P<0.05). However, when the birds were fed with the NC diet + Amilase (AMY)+Xilanase (XYL), the BWG improved and reached the same level as the Positive Control (PC). Trial 2- The birds received enzymes supplementation in the total phase and others only in the grower phase.The ME reduction by 120 kcal/kg increased the feed conversion ratio (FCR) (P<0.05) when compared to PC and none of the enzyme programs was able to recover the performance. It can be concluded that it is possible to supplement with AMY...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Carbohydrase; Enzymes; Amylase; Xylanase.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700030
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Estrategias nutricionales para mejorar la respuesta productiva de cerdos en engorda. Colegio de Postgraduados
Martínez Aispuro, José Alfredo.
El presente trabajo tuvo como objetivo reducir y/u optimizar la concentración de algunos nutrimentos en las dietas de cerdos de engorda que puede resultar benéfica en la formulación de raciones. Se realizaron 3 experimentos para evaluar el nivel de lisina, el uso de alcaloides y xilanasas en dietas para cerdos de engorda. En el experimento 1 se determinaron los niveles óptimos biológicos (NOB) de lisina total para cerdos en las etapas de crecimiento y finalización en el comportamiento productivo, las características de la canal, y la concentración de urea en plasma. El NOB para ganancia diaria de peso (GDP), conversión alimenticia, y ganancia de carne magra fue 0.88% de lisina total; mientras que el NOB para consumo de alimento fue 0.961% de lisina total....
Palavras-chave: Lisina; Alcaloides; Dietas con baja proteína; Xilanasas; Energía; Proteína; Lysine; Alkaloids; Low protein diets; Xylanase; Energy; Protein; Ganadería; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2159
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Exogenous enzymes improve performance and carcass traits of feedlot cattle fed high-grain diet R. Bras. Zootec.
Neumann,Mikael; Leão,Guilherme Fernando Mattos; Horst,Egon Henrique; Stuani,Osmair Flavio; Sangali,Cleiton Pagliari; Castilho,Ricardo.
ABSTRACT The objective of this study was to evaluate the effects of supplementation of two different enzymatic complexes, fibrolytic (NSPases) or amylolytic (EXP3066) enzymes, on growth performance, digestibility, behavior, and carcass characteristics of feedlot cattle. Thirty-six ½ Angus yearling bulls with an average initial weight of 391±5.0 kg were used in this experiment blocked by weight in a completely randomized design divided in three treatments: control, NSPases, and EXP3066. The basal diet was composed of 85% whole corn grain and 15% nucleus. NSPases increased average daily gain on days 63 and 84, and gain:feed ratio on days 42, 63, and 84. Total carcass weight and carcass daily gain were improved by 4.8% and up to 6.0% with EXP3066 and NSPases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Corn; High-energy diet; Non-starch polysaccharides; Xylanase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100611
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Fermentation characteristics in hay from Cynodon and crop stubble treated with exogenous enzymes Rev. Ciênc. Agron.
Santana,Yânez André Gomes; Vasconcelos,Vânia Rodrigues; Alves,Arnaud Azevêdo; Silva,Suzana Coimbra de Moura Lustosa e; Garcez,Bruno Spindola.
ABSTRACT The effect of treatment with xylanase and β-glucanase was evaluated for gas production and the ruminal degradation of nutrients from the hay of Tifton 85 grass and the stubble of maize, sorghum, peanut, sunflower and sesame crops. Two commercial fibrolytic enzymes were used (Dyadic xylanase PLUS - Xylanase; BrewZyme LP-β-glucanase), added to the hay at doses of 7.5 units of endoglucanase and 0.46 units of xylanase per 500 mg/gDM, for the cellulase and xylanase products respectively. The chemical composition of the hay was determined for no enzyme application and 24 hours after enzyme treatment, and the in vitro gas production and in situ microbial degradation was estimated for dry matter, organic matter, neutral detergent fibre and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis hypogaea; Β-glucanase; Helianthus annuus; Sesamum indicum; Sorghum bicolor; Xylanase.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902018000100167
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Hydrolysis of xylans by enzyme systems from solid cultures of Trichoderma harzianum strains BJMBR
Silveira,F.Q.P.; Ximenes,F.A.; Cacais,A.O.G.; Milagres,A.M.F.; Medeiros,C.L.; Puls,J.; Filho,E.X.F..
Xylanase activity was isolated from crude extracts of Trichoderma harzianum strains C and 4 grown at 28oC in a solid medium containing wheat bran as the carbon source. Enzyme activity was demonstrable in the permeate after ultrafiltration of the crude extracts using an Amicon system. The hydrolysis patterns of different xylans and paper pulps by xylanase activity ranged from xylose, xylobiose and xylotriose to higher xylooligosaccharides. A purified ß-xylosidase from the Trichoderma harzianum strain released xylose, xylobiose and xylotriose from seaweed, deacetylated, oat spelt and birchwood xylans. The purified enzyme was not active against acetylated xylan and catalyzed the hydrolysis of xylooligosaccharides, including xylotriose, xylotetraose and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Trichoderma; Xylan; Xylanase.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800003
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Hyperexpression of two Aspergillus niger xylanase genes in Escherichia coli and characterization of the gene products BJM
Yi,Xiuli; Shi,Yan; Xu,Hui; Li,Wei; Xie,Jie; Yu,Rongqing; Zhu,Jun; Cao,Yi; Qiao,Dairong.
The analysis of individual gene product should enable to clarify the role of a particular enzyme in a complex xylanase system of A. niger. The two genes encoding precursors of co-produced endo-1,4-β-D-xylanases, xynA1 and xynB, were isolated from Aspergillus niger SCTCC 400264 (SCTCC, China) by using RT-PCR technique and then successfully expressed in Escherichia coli BL21. The nucleotide sequences of the xynA1 and xynB genes revealed that they were only 52.5% homology to each other. Characterization of the recombinant enzymes revealed the different properties: the specific activity of recombinant XYNA1 was 16.58 U/mg compared to 1201.7 U/mg for recombinant XYNB; The optimum temperature and pH of the recombinant XYNA1 were 35 ºC and 3.0, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Xylanase; Prokaryotic expression; Enzymatic characterization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300030
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Improvement of xylanase production by Cochliobolus sativus in solid state fermentation BJM
Bakri,Yasser; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cochliobolus sativus; Xylanase; Solid-state fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300036
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Induction of xylanase in thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile BABT
Joshi,Chetna; Khare,Sunil Kumar.
Regulation of xylanase production in two thermophilic fungi Scytalidium thermophilum and Sporotrichum thermophile was investigated. The expression of xylanase was found to be inducible in both the cases. Various carbon sources were tested so as to identify the inducers. Soy flour and oat spelt xylan induced maximum level of xylanase in Scytalidium thermophilum and Sporotrichum thermophile respectively. Induction of xylanase in Scytalidium thermophilum led to simultaneous induction of cellulase. The zymography of enzyme preparations revealed that different carbon sources caused differential expression of multiple isoforms of xylanase in Scytalidium thermophilum, but same isoforms were expressed by Sporotrichum thermophile irrespective of the carbon source...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Induction; Isoforms; Xylanase; Biobleaching.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100003
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Influence of agitation speeds and aeration rates on the Xylanase activity of Aspergillus niger SS7 BABT
Bakri,Yasser; Mekaeel,Alaa; Koreih,Amal.
In this study, the effect of agitation and aeration rates on xylanase activity of Aspergillus niger SS7 in 3-litre stirred tank bioreactor was investigated. The agitation rates tested were 100, 200 and 300 rpm at each airflow rates of 0.5, 1.0 and 1.5 vvm. The maximum xylanase activity in mono- agitator system was at the agitation speed of 200 rpm and aeration rate of 1.0 vvm. In bi-agitator system, at low agitation speed (100 rpm), the xylanase activity was enhanced by 13% compared to mono- agitator system for an aeration rate of 1.0 vvm. Xylanase productivity in continuous culture was higher by approximately 3.5 times than in batch culture.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Agitation; Aeration; Xylanase.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400003
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Influence of xylanase addition on the characteristics of loaf bread prepared with white flour or whole grain wheat flour Ciênc. Tecnol. Aliment.
Jaekel,Leandra Zafalon; Silva,Camila Batista da; Steel,Caroline Joy; Chang,Yoon Kil.
The aim of this study was to verify the influence of the addition of the enzyme xylanase (four concentrations: 0, 4, 8, and 12 g.100 kg-1 flour) on the characteristics of loaf bread made with white wheat flour or whole grain wheat flour. Breads made from white flour and added with xylanase had higher specific volumes than those of the control sample (no enzyme); however, the specific volume did not differ significantly (p < 0.05) among the breads with different enzyme concentrations. All formulations made from whole grain wheat flour and added with xylanase also had specific volumes significantly higher than those of the control sample, and the highest value was found for the 8 g xylanase.100 kg-1 flour formulation. With respect to moisture content, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: White wheat flour; Whole grain wheat flour; Xylanase; Loaf bread.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400030
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Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3 BABT
Isil,Seyis; Nilufer,Aksoz.
In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60°C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylan; Xylanase; Lignocellulosic material; Enzyme stability; Metal ions.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004
Registros recuperados: 42
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