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Registros recuperados: 11
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Action mechanisms involved in the bioprotective effect of Lactobacillus harbinensis K.V9.3.1.Np against Yarrowia lipolytica in fermented milk ArchiMer
Mieszkin, Sophie; Hymery, Nolwenn; Debaets, Stella; Coton, Emmanuel; Le Blay, Gwenaelle; Valence, Florence; Mounier, Jerome.
The use of lactic acid bacteria (LAB) as bioprotective cultures can be an alternative to chemical preservatives or antibiotic to prevent fungal spoilage in dairy products. Among antifungal LAB, Lactobacillus harbinensis K.V9.3.1Np showed a remarkable antifungal activity for the bioprotection of fermented milk without modifying their organoleptic properties (Delavenne et al., 2015). The aim of the present study was to elucidate the action mechanism of this bioprotective strain against the spoilage yeast Yarrowia lipolytica. To do so, yeast viability, membrane potential, intracellular pH (pHi) and reactive oxygen species (ROS) production were assessed using flow cytometry analyses after 3, 6 and 10 days incubation in cell-free supernatants. The tested...
Tipo: Text Palavras-chave: Lactobacillus harbinensis; Yarrowia lipolytica; Bioprotection; Antifungal; Action mechanisms; Dairy products.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48390/48563.pdf
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Bioconversion of R-(+)-limonene to perillic acid by the yeast Yarrowia lipolytica BJM
Ferrara,Maria Antonieta; Almeida,Débora S.; Siani,Antonio C.; Lucchetti,Leonardo; Lacerda,Paulo S.B.; Freitas,André; Tappin,Marcelo R.R.; Bon,Elba P.S..
Perillyl derivatives are increasingly important due to their flavouring and antimicrobial properties as well as their potential as anticancer agents. These terpenoid species, which are present in limited amounts in plants, may be obtained via bioconversion of selected monoterpene hydrocarbons. In this study, seventeen yeast strains were screened for their ability to oxidize the exocyclic methyl group in the p-menthene moiety of limonene into perillic acid. Of the yeast tested, the highest efficiency was observed for Yarrowia lipolytica ATCC 18942. The conversion of R (+)-limonene by Y. lipolytica was evaluated by varying the pH (3 to 8) and the temperature (25 to 30 ºC) in a reaction medium containing 0.5% v/v limonene and 10 gµL of stationary phase cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yarrowia lipolytica; Bioconversion; Limonene; Perillic acid.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400008
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Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants BJM
Rakicka,Magdalena; Rywińska,Anita; Cybulski,Krzysztof; Rymowicz,Waldemar.
Abstract Various chemical compounds, including surfactants, when introduced to culture media may increase the permeability of cellular membranes and thereby affect the quantity of metabolites excreted by cells. The aim of the present study was to evaluate the impact of detergents including Triton X-100, Span 20 and Tween 80 on erythritol production from glycerol by Yarrowia lipolytica Wratislavia K1 in a shake-flask experiment, batch and fed-batch cultures. When Span 20 was added to a fed-batch culture with glycerol as a carbon source (300 g L-1), erythritol production increased by 15% compared to the culture without the surfactant where it reached 142 g L-1 after 5 days, which corresponded to 0.47 g g-1 yield and productivity of 1.1 g L-1 h-1. Therefore,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Erythritol; Glycerol; Span 20; Surfactants; Yarrowia lipolytica.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200417
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Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis BJM
Santos,Elisane Odriosolla dos; Michelon,Mariano; Furlong,Eliana Badiale; Burkert,Janaína Fernandes de Medeiros; Kalil,Susana Juliano; Burkert,Carlos André Veiga.
The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Raw glycerol; Single cell protein; Yeast biomass; Yarrowia lipolytica.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200002
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Factors influencing crude oil biodegradation by Yarrowia lipolytica BABT
Ferreira,Tatiana Felix; Coelho,Maria Alice Zarur; Rocha-Leão,Maria Helena Miguez da.
Yarrowia lipolytica is unique strictly aerobic yeast with the ability to efficiently degrade hydrophobic substrates such as n-alkenes, fatty acids, glycerol and oils. In the present work, a 2(4) full factorial design was used to investigate the influence of the independent variables of temperature, agitation, initial cell concentration and initial petroleum concentration on crude oil biodegradation. The results showed that all variables studied had significant effects on the biodegradation process. Temperature, agitation speed and initial cell concentration had positive effects, and initial petroleum concentration had a negative effect. Among the crude oil removal conditions studied, the best temperature and agitation conditions were 28ºC and 250 rpm,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yarrowia lipolytica; Crude oil; Biodegradation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500019
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Green (Detox) juice physicochemical properties and stabilization effect of naturals emulsifiers Ciência Rural
Fraga,Jully Lacerda; Sant’Ana,Gizele Cardoso Fontes; Silva,Kelly Alencar; Amaral,Priscilla Filomena Fonseca.
ABSTRACT: Green or “detox” juice is a mixture of fruit juice with vegetables, which has been used intensively by consumers seeking for healthy food. Physicochemical properties of Green juice were accessed in the present research, which brings new insights for the use of this beverage in human diet. A total phenolic content of 2833.60 mg GAE (Gallic acid equivalent)/ g of juice and a Total Antioxidant Capacity by FRAP of 323.62 µM Fe2SO4 / g of juice and by ABTS•+ of 333.11 µM Trolox/ g of juice, indicated good antioxidant properties. Low energy and reducing sugar content indicate its use for low calorie diet, but low carbohydrate and protein content prove that Green juice cannot be used as meal replacement. The addition of a microbial biosurfactant (YlBio)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yarrowia lipolytica; Chia seed mucilage; Food rheology; Pseudoplastic fluid; Bioemulsifiers.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300752
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Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica Electron. J. Biotechnol.
Papanikolaou,Seraphim; Chevalot,Isabelle; Galiotou-Panayotou,Maria; Komaitis,Michael; Marc,Ivan; Aggelis,George.
The aim of the present study was to assess the potential of valorisation of a solid industrial derivative of tallow, composed of saturated free-fatty acids ("stearin"), by fermentations carried out by the yeast Yarrowia lipolytica ACA-DC 50109 in order to produce microbial lipid, biomass and extra-cellular lipase. High quantities of biomass were produced (biomass yield of around 1.1 ± 0.1 g of total biomass produced per g of fat consumed) when the organism was grown in shake flasks, regardless of the concentration of extra-cellular nitrogen present. Cellular lipids accumulated in notable quantities regardless of the nitrogen availability of the medium, though this process was clearly favoured at high initial fat and low initial nitrogen concentrations. The...
Tipo: Journal article Palavras-chave: Lipase; Microbial lipids; Single-cell protein; Tallow; Yarrowia lipolytica.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300010
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Produção de lipase intracelular por Yarrowia lipolytica utilizando óleo de fritura residual como indutor. Repositório Alice
NUNES, P. M. B.; AMARAL, P. F. F.; ROCHA-LEÃO, M. H. M.; BRIGIDA, A. I. S..
2013
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Lipase; Yarrowia lipolytica.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/967978
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Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus) BJM
Imandi,Sarat Babu; Karanam,Sita Kumari; Garapati,Hanumantha Rao.
Mustard oil cake (Brassica napus), the residue obtained after extraction of mustard oil from mustard oil seeds, was investigated for the production of lipase under solid state fermentation (SSF) using the marine yeast Yarrowia lipolytica NCIM 3589. Process parameters such as incubation time, biomass concentration, initial moisture content, carbon source concentration and nitrogen source concentration of the medium were optimized. Screening of ten nitrogen and five carbon sources has been accomplished with the help of Plackett-Burman design. The highest lipase activity of 57.89 units per gram of dry fermented substrate (U/gds) was observed with the substrate of mustard oil cake in four days of fermentation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Mustard oil cake; Plackett-Burman design; Yarrowia lipolytica.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300041
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Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica ArchiMer
Dulermo, Remi; Brunel, Francois; Dulermo, Thierry; Ledesma-amaro, Rodrigo; Vion, Jeremy; Trassaert, Marion; Thomas, Stephane; Nicaud, Jean-marc; Leplat, Christophe.
Background The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2. Results To our knowledge, our study is the first to use a set of vectors with promoters of variable strength to produce proteins of industrial interest. We used the more conventional TEF and hp4d promoters along with five new hybrid promoters: 2UAS1-pTEF,...
Tipo: Text Palavras-chave: Yarrowia lipolytica; Protein production; RedStar2; Glucoamylase; Xylanase; Hybrid promoters.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00428/54003/55193.pdf
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Valorization of raw glycerol for citric acid production by Yarrowia lipolytica yeast Electron. J. Biotechnol.
Rywinska,Anita; Rymowicz,Waldemar; Marcinkiewicz,Marta.
In the present report, citric acid production from raw glycerol in two fed-batch systems by acetate negative-mutants of Yarrowia lipolytica: Wratislavia 1.31 and Wratislavia AWG7 was compared. In the system, in which the total glycerol concentration was 200 g∙L-1, the substrate was added by pulsed additions, and in the other, in which the total glycerol concentration was 300 g∙L-1, the substrate was added at a constant feeding rate of 1.4 g∙h-1. Despite high citric acid concentrations (155.2 and 157.5 g∙L-1 with Y. lipolytica Wratislavia 1.31 and Y. lipolytica Wratislavia AWG7, respectively) obtained from 300 g∙L-1 of glycerol, the yield of citric acid was similar, i.e. about 0.6 g∙g-1. The...
Tipo: Journal article Palavras-chave: Citric acid; Fed-batch culture; Yarrowia lipolytica; Raw glycerol.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400009
Registros recuperados: 11
Primeira ... 1 ... Última
 

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