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100 años de Chagas (1909-2009): revisión, balance y perspectiva Rev. Soc. Entomol. Argent.
Sanmartino,Mariana.
Cien años han transcurrido desde las primeras publicaciones de Carlos Chagas acerca de la enfermedad que lleva su nombre, del agente causal y de los insectos vectores. A propósito de tal conmemoración, el presente artículo pretende dar una breve revisión de ciertas cuestiones históricas, una mirada crítica a la situación actual y algunas pistas para el camino que queda por recorrer.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enfermedad de Chagas; Triatominae; Historia; Investigaciones; Desafíos.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0373-56802009000200001
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123-I ioflupane (Datscan®) presynaptic nigrostriatal imaging in patients with movement disorders BABT
Soriano Castrejón,Angel; García Vicente,Ana María; Cortés Romera,Montserrat; Cano,Julia Vaamonde; Marina,Sonia Rodado; Poblete García,Victor Manuel; Ruiz Solís,Sebastián; Talavera Rubio,María del Prado.
123-I Ioflupane (Datscan®) presynaptic imaging has been shown to have a significant utility in the assessment of patients with movement disorders 123-I Ioflupane SPECT is able to distinguish between Parkinson’s disease (PD) and other forms of parkinsonism without degeneration of the nigrostriatal pathway, including a common movement disorder such as essential tremor, and to assess disease progression in PD and other neurodegenerative disorders involving the substantia nigra.
Tipo: Info:eu-repo/semantics/article Palavras-chave: 123-I Ioflupane; Dopamine transporter imaging; Movement disorders; Parkinson’s disease.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700017
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1,25-dihidroxivitamina-D 3 sobre as características ósseas de frangos de corte fêmeas Arq. Bras. Med. Vet. Zootec.
Vieites,F.M.; Drosghic,L.C.A.B.; Souza,C.S.; Lima,C.A.R.; Moraes,G.H.K.; Nunes,R.V.; Vasconcellos,C.H.F.; Vargas Júnior,J.G..
RESUMO Objetivou-se avaliar as características dos tibiotarsos de frangos de corte fêmeas. As variáveis analisadas foram os pesos in natura, secos e desengordurados, o comprimento, os diâmetros, a resistência óssea, o índice de Seedor (IS), os percentuais de proteínas colagenosas (PC), as proteínas não colagenosas (PNC), os minerais (cálcio, fósforo, potássio e sódio) e as cinzas. Foram utilizadas 648 aves, da marca comercial Cobb®, em um delineamento em blocos ao acaso, com seis tratamentos e seis repetições, com 18 aves por unidade experimental. Os tratamentos consistiram na suplementação de 0,00; 0,50; 1,00; 1,50; 2,00 e 2,50 μg de 1,25-dihidroxivitamina-D3 (1,25(OH)2D3)/kg de ração. Aos 21 e 35 dias de idade, as aves foram pesadas e uma ave por unidade...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colágeno; Índice de Seedor; Resistência à quebra; Solanum glaucophyllum.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000501285
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131I-induced changes in rat thyroid gland function BJMBR
Torlak,V.; Zemunik,T.; Modun,D.; Capkun,V.; Pesutic-Pisac,V.; Markotic,A.; Pavela-Vrancic,M.; Stanicic,A..
Therapeutic doses of 131I administered to thyrotoxic patients may cause thyroid failure. The present study used a rat model to determine thyroid function after the administration of different doses of 131I (64-277 µCi). Thirty male Fisher rats in the experimental group and 30 in the control group (untreated) were followed for 6 months. The animals were 4 months old at the beginning of the experiment and were sacrificed at an age of 9 months. Hormone concentration was determined before 131I administration (4-month-old animals) and three times following 131I administration, when the animals were 7, 8, and 9 months old. The thyroid glands were removed and weighed, their volume was determined and histopathological examination was performed at the end of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroid gland; 131I; Hormones; Histopathology; T3; T4; TSH.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800009
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1,3,4-Thiadiazole and 1,2,4-triazole-3(4 H )-thione bearing salicylate moiety: synthesis and evaluation as anti- Candida albicans BJPS
Radwan,Awwad Abdoh; Alanazi,Fares Kaed; Al-Agamy,Mohammed Hamed.
Abstract Dramatically increased occurrence of both superficial and invasive fungal infections has been observed. Candida albicans appear to be the main etiological agent of invasive fungal infections. The anti-C. albicans activity of thiosemicarbazide, 1,3,4-Thiadiazole, and 1,2,4-triazole-3(4H)-thione compounds (compounds 3-23) were investigated. The MIC values of thiadiazole and triazole derivatives 10-23 were in the range of 0.08-0.17 µmol mL-1, while that of fluconazole was 0.052 µmol mL-1. Compound 11 (5-(2-(4-chlorobenzyloxy)phenyl)-N-allyl-1,3,4-thiadiazol-2-amine) and compound 18 (5-(2-(4-chlorobenzyloxy)phenyl)-4-allyl-2H-1,2,4-triazole-3(4H)-thione) were found to be the most active compounds, with MIC values of 0.08 µmol mL-1. The newly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anti-Candida/docking studies; Thiadiazole; Triazole.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100614
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13C NMR spectral data and molecular descriptors to predict the antioxidant activity of flavonoids BJPS
Scotti,Luciana; Fernandes,Mariane Balerine; Muramatsu,Eric; Emereciano,Vicente de Paula; Tavares,Josean Fechine; Silva,Marcelo Sobral da; Scotti,Marcus Tullius.
Tissue damage due to oxidative stress is directly linked to development of many, if not all, human morbidity factors and chronic diseases. In this context, the search for dietary natural occurring molecules with antioxidant activity, such as flavonoids, has become essential. In this study, we investigated a set of 41 flavonoids (23 flavones and 18 flavonols) analyzing their structures and biological antioxidant activity. The experimental data were submitted to a QSAR (quantitative structure-activity relationships) study. NMR 13C data were used to perform a Kohonen self-organizing map study, analyzing the weight that each carbon has in the activity. Additionally, we performed MLR (multilinear regression) using GA (genetic algorithms) and molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Flavonoids/antioxidant activity; 13C NMR; Kohonen self-organizing map.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000200005
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13CO2 recovery fraction in expired air of septic patients under mechanical ventilation BJMBR
Martins,M.A.; Coletto,F.A.; Martins-Filho,O.A.; Marchini,J.S.; Basile-Filho,A..
The continuous intravenous administration of isotopic bicarbonate (NaH13CO2) has been used for the determination of the retention of the 13CO2 fraction or the 13CO2 recovered in expired air. This determination is important for the calculation of substrate oxidation. The aim of the present study was to evaluate, in critically ill patients with sepsis under mechanical ventilation, the 13CO2 recovery fraction in expired air after continuous intravenous infusion of NaH13CO2 (3.8 µmol/kg diluted in 0.9% saline in ddH2O). A prospective study was conducted on 10 patients with septic shock between the second and fifth day of sepsis evolution (APACHE II, 25.9 ± 7.4). Initially, baseline CO2 was collected and indirect calorimetry was also performed. A primer of 5 mL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recovery fraction; 13CO2; Stable isotope; Septic shock; Intensive care setting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000700003
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1,3-Diarilpropanos de Iryanthera paraensis e Iryanthera tricornis Acta Amazonica
Silva,José Jerônimo da; Giesbrecht,Astréa M.; Alvarenga,Marden A. de; Gottlieb,Otto R..
Iryanthera paraensis e I. tricornis (Myristicaceae) contêm na madeira de seus troncos 1-(2', 4' - dihidroxifenil) -3- (4", 5"-metilenodoxi-2" -metoxifenil) -propano (1a 1-(2', 4' dihidroxifenil) -3-(3", 4" -metilenodioxifenil)-propano (1b) e 1-(4' -hidroxi -2'-metoxifenil) -3-(4"-hidroxi-3"-metoxifenil) -propano (1c). Apenas da primeira espécie se isolou adicionalmente 1-(2', 4'-dihidroxi-3' -metilfenil)-3-(4", 5"-metilenodioxi-2"- metoxifenil) -propano (1d) e 1-(2'-hidroxi-4'metoxifenil) -3-(3", 4"-metilenodioxifenil) -propano (1e). Dados espectroscópicos para a substância 1b, são descritos pela primeira vez.
Tipo: Info:eu-repo/semantics/article
Ano: 1984 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59671984000200455
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1,4- Addition of diazomethane to a heterodiene: a direct preparation of the oxazolic ring Anais da ABC (AABC)
Emery,Flávio da S.; Silva,Raphael S.F.; Moura,Kelly C.G. de; Pinto,Maria C.F.R.; Amorim,Mauro B.; Malta,Valéria R.S.; Santos,Regina H.A.; Honório,Kátia M.; Silva,Albérico B.F. da; Pinto,Antonio V..
The reaction of naphthoquinone-oximes (3) and (4) with diazomethane yields directly, in one step, the oxazoles (5) and (6), respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quinones; Oximes; Diazomethane; Oxazoles.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100004
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14-3-3 proteins in apoptosis BJMBR
Rosenquist,M..
The once obscure members of the 14-3-3 protein family play significant roles in the determination of cell fate. By inhibiting the pro-apoptotic BAD (Bcl-2-antagonist of cell death) and the transcription factor FKHRL-1, 14-3-3 displays important anti-apoptotic characteristics. To date, five points of interaction of 14-3-3 with the apoptotic machinery have been identified. How these interactions are regulated still remains a mystery.
Tipo: Info:eu-repo/semantics/article Palavras-chave: 14-3-3; Apoptosis; Programmed cell death.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400001
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15N uptake fom manure and fertilizer sources by three consecutive crops under controlled conditions Rev. Bras. Ciênc. Solo
Garza,Héctor Mario Quiroga; Delgado,Jorge A.; Wong,José Antonio Cueto; Lindemann,William C..
There are several regions of the world where soil N analysis and/or N budgets are not used to determine how much N to apply, resulting in higher than needed N inputs, especially when manure is used. One such region is the North Central "La Comarca Lagunera", one of the most important dairy production areas of Mexico. We conducted a unique controlled greenhouse study using 15N fertilizer and 15N isotopic-labeled manure that was labeled under local conditions to monitor N cycling and recovery under higher N inputs. The manure-N treatment was applied only once and was incorporated in the soil before planting the first forage crop at an equivalent rate of 30, 60 and 120 Mg ha-1 dry manure. The 15N treatments were equivalent to 120 and 240 kg ha-1 (NH4)2SO4-N...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Denitrification; Nitrogen use efficiency; Annual ryegrass; Salinity; Sudangrass.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000500018
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15N-labed glycine synthesis Anais da ABC (AABC)
Tavares,Claudinéia R.O.; Bendassolli,José A.; Coelho,Fernando; Sant'ana Filho,Carlos R.; Prestes,Clelber V..
This work describes a method for 15N-isotope-labeled glycine synthesis, as well as details about a recovery line for nitrogen residues. To that effect, amination of alpha-haloacids was performed, using carboxylic chloroacetic acid and labeled aqueous ammonia (15NH3). Special care was taken to avoid possible 15NH3 losses, since its production cost is high. In that respect, although the purchase cost of the 13N-labeled compound (radioactive) is lower, the stable tracer produced constitutes an important tool for N cycling studies in living organisms, also minimizing labor and environmental hazards, as well as time limitation problems in field studies. The tests were carried out with three replications, and variable 15NH3(aq) volumes in the reaction were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stable isotope; Glycine; Amino acid.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300005
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15N-labeled nitrogen from green manure and ammonium sulfate utilization by the sugarcane ratoon Scientia Agricola
Ambrosano,Edmilson José; Trivelin,Paulo Cesar Ocheuze; Cantarella,Heitor; Ambrosano,Gláucia Maria Bovi; Schammass,Eliana Aparecida; Muraoka,Takashi; Rossi,Fabrício.
Legumes as green manure are alternative sources of nitrogen (N) for crops and can supplement or even replace mineral nitrogen fertilization due to their potential for biological nitrogen fixation (BNF). The utilization of nitrogen by sugarcane (Saccharum spp.) fertilized with sunn hemp (Crotalaria juncea L.) and ammonium sulfate (AS) was evaluated using the 15N tracer technique. N was added at the rate of 196 and 70 kg ha-1 as 15Nlabeled sunn hemp green manure (SH) and as ammonium sulfate (AS), respectively. Treatments were: (i) Control; (ii) AS15N; (iii) SH15N + AS; (iv) SH15N; and (v) AS15N + SH. Sugarcane was cultivated for five years and was harvested three times. 15N recovery was evaluated in the two first harvests. In the sum of the three harvests,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum spp.; Brazil; Sunn hemp; Isotope technique.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000300014
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16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting BJM
Navarro-Noya,Yendi; Hernández-Rodríguez,César; Zenteno,Juan C; Buentello-Volante,Beatriz; Cancino-Díaz,Mario E; Jan-Roblero,Janet; Cancino-Díaz,Juan C.
Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophthalmitis; Staphylococcus epidermidis; PCR; DGGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
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16S rRNA gene-based identification of microbiota associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga (Diptera, Psychodidae) from central Amazon, Brazil BJM
Sousa,Katianne Barbosa Alves de; Silva,Túllio Romão Ribeiro da; Alencar,Ronildo Baiatone; Baton,Luke Anthony; Naveca,Felipe Gomes; Shimabukuro,Paloma Helena Fernandes.
Bacteria associated with the parthenogenetic troglobiont sand fly Deanemyia maruaga were characterized by sequencing cloned 16S rDNA PCR products. Eleven novel partial 16S rDNA sequences, with varying degrees of similarity to Actinobacteria, were identified. None of the sequences identified had homology to those known from parthenogenesis-inducing bacteria.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacteria; Bat guano; Parthenogensis; Sand flies; Troglobiont.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100047
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16S rRNA region based PCR protocol for identification and subtyping of Parvimonas micra BJM
Ota-Tsuzuki,C.; Brunheira,A.T.P.; Mayer,M.P.A..
The present study established a PCR protocol in order to identify Parvimonas micra and to evaluate the intra-species diversity by PCR-RFLP of 16S rRNA partial sequence. The data indicated that the protocol was able to identify this species which could be clustered in five genotypes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR-RFLP; 16S rRNA; Anaerobic cocci.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400001
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16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains BJM
Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
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17,20β-P and cortisol are the main in vitro metabolites of 17-hydroxy-progesterone produced by spermiating testes of Micropogonias furnieri (Desmarest, 1823) (Perciformes: Sciaenidae) Neotropical Ichthyology
Vizziano Cantonnet,Denise; Mateo,Magdalena; Alberro,Andrés; Barrios,Florencia; Fostier,Alexis.
The aim was to investigate the major C21 steroids produced by spermiating white croaker Micropogonias furnieri (Sciaenidae) in order to establish the potential mediator of gamete maturation in males of this species. The testes steroid production at the spawning season was identified incubating the 3H-17-hydroxy-4-pregnene-3,20-dione precursor through thin layer chromatography, high pressure liquid chromatography, enzymatic oxydation, acetylation and immunochemistry analyses. 17,20β-Dihydroxy-4-pregnen-3-one (17,20β-P) and 11β,17,21-Trihydroxy-4-pregnene-3,20-dione (cortisol) were the main metabolites produced. Contrary to what we expected, 17,20β,21-Trihydroxy-4-pregnen-3-one was not detected. Circulating levels of 17,20β-P were undetectable in immature...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Male gonad; Maturing steroids; Reproduction; Steroid synthesis; White croaker.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000300613
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18S-rDNA SEQUENCING, ENZYME PATTERNS AND MORPHOLOGICAL CHARACTERIZATION OF TRICHOPHYTON ISOLATES BJM
Nascimento,Adriana Mendes do; Martinez-Rossi,Nilce M..
Dermatophytes, capable to use keratin of the host for nutrition, belong to one of the major groups of pathogenic fungi. Since dermatophytes are a closely related group they share various common features, and the morphology of isolates of a given species can be atypical, making species identification and differentiation even more difficult. Many methods have been explored in attempts to distinguish dermatophytes, but the combined use of different approaches for the investigation of the intraspecific and interspecific variability of Trichophyton continues to be scarce. Some studies have shown that amplified fragments of the small ribosomal DNA subunit 18S contains variable regions which can be used to discriminate between medically relevant yeast species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichophyton; 18S-rDNA sequencing; Extracellular enzymes; Dermatophyte.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300004
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1971-1991: twenty years of marine ecology OMA
Heip, C.H.R..
Tipo: Info:eu-repo/semantics/article
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2862
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